Colorimetric method as alternative to chromium (III) quantification in cattle feces
One of the main factors to establish productivity of grazing cattle is the estimation of forage intake. For this, the most widely used technique is based on the estimation of fecal output using chromium dioxide as external marker. However, quantification can be expensive and sometimes not precise du...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Editora da Universidade Estadual de Maringá (Eduem)
2020-04-01
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| Series: | Acta Scientiarum: Animal Sciences |
| Subjects: | |
| Online Access: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/48096 |
| _version_ | 1831750619405221888 |
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| author | Alexandra Torres Ospina Julian Andrés Zapata Jesus Humberto Gil Gonzalez Luis Giraldo Diana Marcela Valencia |
| author_facet | Alexandra Torres Ospina Julian Andrés Zapata Jesus Humberto Gil Gonzalez Luis Giraldo Diana Marcela Valencia |
| author_sort | Alexandra Torres Ospina |
| collection | DOAJ |
| description | One of the main factors to establish productivity of grazing cattle is the estimation of forage intake. For this, the most widely used technique is based on the estimation of fecal output using chromium dioxide as external marker. However, quantification can be expensive and sometimes not precise due to the methodology used for this purpose. Therefore, the aim of this study was to validate the colorimetric method for chromium quantification and to implement it in the estimation of fecal output in grazing cattle. The temperature, the digestion time and the wavelength for the measurement were evaluated. The method was validated for selectivity, linearity, detection and quantification limits, precision, accuracy, and stability. Results showed that temperature and digestion time are critical to improve sensitivity and quantification limits. The validation demonstrated that the method is suitable for the quantification of Cr2O3 in a wide range of concentrations, being statistically comparable with a reference method, and offering a reliable low cost and easy to implement alternative, to estimate fecal output in bovine digestibility studies. |
| first_indexed | 2024-12-21T22:30:52Z |
| format | Article |
| id | doaj.art-e728d6da5d2740f0a1b072b3afc12067 |
| institution | Directory Open Access Journal |
| issn | 1806-2636 1807-8672 |
| language | English |
| last_indexed | 2024-12-21T22:30:52Z |
| publishDate | 2020-04-01 |
| publisher | Editora da Universidade Estadual de Maringá (Eduem) |
| record_format | Article |
| series | Acta Scientiarum: Animal Sciences |
| spelling | doaj.art-e728d6da5d2740f0a1b072b3afc120672022-12-21T18:48:06ZengEditora da Universidade Estadual de Maringá (Eduem)Acta Scientiarum: Animal Sciences1806-26361807-86722020-04-0142e48096e4809610.4025/actascianimsci.v42i1.4809648096Colorimetric method as alternative to chromium (III) quantification in cattle fecesAlexandra Torres Ospina0Julian Andrés Zapata1Jesus Humberto Gil Gonzalez2Luis Giraldo3Diana Marcela Valencia4Universidad Nacional de ColombiaUniversidad de AntioquiaUniversidad Nacional de ColombiaUniversidad Nacional de ColombiaCentro de Investigación el NusOne of the main factors to establish productivity of grazing cattle is the estimation of forage intake. For this, the most widely used technique is based on the estimation of fecal output using chromium dioxide as external marker. However, quantification can be expensive and sometimes not precise due to the methodology used for this purpose. Therefore, the aim of this study was to validate the colorimetric method for chromium quantification and to implement it in the estimation of fecal output in grazing cattle. The temperature, the digestion time and the wavelength for the measurement were evaluated. The method was validated for selectivity, linearity, detection and quantification limits, precision, accuracy, and stability. Results showed that temperature and digestion time are critical to improve sensitivity and quantification limits. The validation demonstrated that the method is suitable for the quantification of Cr2O3 in a wide range of concentrations, being statistically comparable with a reference method, and offering a reliable low cost and easy to implement alternative, to estimate fecal output in bovine digestibility studies.http://www.periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/48096spectrophotometry; fecal output; external marker; chromium dioxide; validation. |
| spellingShingle | Alexandra Torres Ospina Julian Andrés Zapata Jesus Humberto Gil Gonzalez Luis Giraldo Diana Marcela Valencia Colorimetric method as alternative to chromium (III) quantification in cattle feces Acta Scientiarum: Animal Sciences spectrophotometry; fecal output; external marker; chromium dioxide; validation. |
| title | Colorimetric method as alternative to chromium (III) quantification in cattle feces |
| title_full | Colorimetric method as alternative to chromium (III) quantification in cattle feces |
| title_fullStr | Colorimetric method as alternative to chromium (III) quantification in cattle feces |
| title_full_unstemmed | Colorimetric method as alternative to chromium (III) quantification in cattle feces |
| title_short | Colorimetric method as alternative to chromium (III) quantification in cattle feces |
| title_sort | colorimetric method as alternative to chromium iii quantification in cattle feces |
| topic | spectrophotometry; fecal output; external marker; chromium dioxide; validation. |
| url | http://www.periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/48096 |
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