WISP2 promotes cell proliferation via targeting ERK and YAP in ovarian cancer cells
Abstract Background Wnt-inducible signaling pathway protein 2 (WISP2) is a wnt1-induced signaling pathway protein 2. Although studies indicate that WISP2 may promote the development of various tumors, its role in ovarian cancer remains unclear. The objective of the current study was to analyze the e...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-07-01
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| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13048-020-00687-8 |
| _version_ | 1797966654475337728 |
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| author | Zi-Qing Shi Zi-Yan Chen Yao Han Heng-Yan Zhu Meng-Dan Lyu Han Zhang Yi Zhang Liu-Qing Yang Wei-Wei Pan |
| author_facet | Zi-Qing Shi Zi-Yan Chen Yao Han Heng-Yan Zhu Meng-Dan Lyu Han Zhang Yi Zhang Liu-Qing Yang Wei-Wei Pan |
| author_sort | Zi-Qing Shi |
| collection | DOAJ |
| description | Abstract Background Wnt-inducible signaling pathway protein 2 (WISP2) is a wnt1-induced signaling pathway protein 2. Although studies indicate that WISP2 may promote the development of various tumors, its role in ovarian cancer remains unclear. The objective of the current study was to analyze the effects of WISP2 on the proliferation and migration of ovarian cancer cells in vitro and in vivo. Results Immunohistochemistry and western blotting indicated that WISP2 was highly expressed in various ovarian cancer tissues and cell lines, but weakly expressed in normal ovary tissue. WISP2 deletion inhibited cell growth, clone formation, and migration of ovarian cancer cells while promoting cell apoptosis and affecting the cell cycle. This growth inhibitory effect caused by WISP2 loss is due to the inhibition of phosphorylated extracellular signal-related kinase (p-ERK)1/2, as well as CCAAT/enhancer-binding protein α (CEBPα) and CEPBβ. In addition, WISP2 deletion also activated the Yes-associated protein (YAP). Conclusion WISP2 deletion inhibits ovarian cancer cell proliferation by affecting ERK signaling pathways. |
| first_indexed | 2024-04-11T02:18:49Z |
| format | Article |
| id | doaj.art-e72adeed9d664e669de944a9bedc4c81 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T02:18:49Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-e72adeed9d664e669de944a9bedc4c812023-01-03T00:29:45ZengBMCJournal of Ovarian Research1757-22152020-07-0113111110.1186/s13048-020-00687-8WISP2 promotes cell proliferation via targeting ERK and YAP in ovarian cancer cellsZi-Qing Shi0Zi-Yan Chen1Yao Han2Heng-Yan Zhu3Meng-Dan Lyu4Han Zhang5Yi Zhang6Liu-Qing Yang7Wei-Wei Pan8School of Medicine, Jiaxing UniversitySchool of Medicine, Jiaxing UniversitySchool of Medicine, Jiaxing UniversitySchool of Medicine, Jiaxing UniversitySchool of Medicine, Jiaxing UniversitySchool of Medicine, Jiaxing UniversitySchool of Medicine, Jiaxing UniversitySchool of Medicine, Jiaxing UniversitySchool of Medicine, Jiaxing UniversityAbstract Background Wnt-inducible signaling pathway protein 2 (WISP2) is a wnt1-induced signaling pathway protein 2. Although studies indicate that WISP2 may promote the development of various tumors, its role in ovarian cancer remains unclear. The objective of the current study was to analyze the effects of WISP2 on the proliferation and migration of ovarian cancer cells in vitro and in vivo. Results Immunohistochemistry and western blotting indicated that WISP2 was highly expressed in various ovarian cancer tissues and cell lines, but weakly expressed in normal ovary tissue. WISP2 deletion inhibited cell growth, clone formation, and migration of ovarian cancer cells while promoting cell apoptosis and affecting the cell cycle. This growth inhibitory effect caused by WISP2 loss is due to the inhibition of phosphorylated extracellular signal-related kinase (p-ERK)1/2, as well as CCAAT/enhancer-binding protein α (CEBPα) and CEPBβ. In addition, WISP2 deletion also activated the Yes-associated protein (YAP). Conclusion WISP2 deletion inhibits ovarian cancer cell proliferation by affecting ERK signaling pathways.http://link.springer.com/article/10.1186/s13048-020-00687-8Ovarian cancerProliferationApoptosisWISP2 |
| spellingShingle | Zi-Qing Shi Zi-Yan Chen Yao Han Heng-Yan Zhu Meng-Dan Lyu Han Zhang Yi Zhang Liu-Qing Yang Wei-Wei Pan WISP2 promotes cell proliferation via targeting ERK and YAP in ovarian cancer cells Journal of Ovarian Research Ovarian cancer Proliferation Apoptosis WISP2 |
| title | WISP2 promotes cell proliferation via targeting ERK and YAP in ovarian cancer cells |
| title_full | WISP2 promotes cell proliferation via targeting ERK and YAP in ovarian cancer cells |
| title_fullStr | WISP2 promotes cell proliferation via targeting ERK and YAP in ovarian cancer cells |
| title_full_unstemmed | WISP2 promotes cell proliferation via targeting ERK and YAP in ovarian cancer cells |
| title_short | WISP2 promotes cell proliferation via targeting ERK and YAP in ovarian cancer cells |
| title_sort | wisp2 promotes cell proliferation via targeting erk and yap in ovarian cancer cells |
| topic | Ovarian cancer Proliferation Apoptosis WISP2 |
| url | http://link.springer.com/article/10.1186/s13048-020-00687-8 |
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