Lipidomic Profiling Reveals Concerted Temporal Patterns of Functionally Related Lipids in <i>Aedes aegypti</i> Females Following Blood Feeding
We conducted a lipidomic analysis of the whole body of female <i>Aedes aegypti</i> mosquitoes at different time points over the course of feeding and reproduction. There were temporal biphasic increases of more than 80% of lipids identified at the time of feeding and from 16 h to 30 h po...
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MDPI AG
2023-03-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/3/421 |
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| author | Meng-Jia Lau Shuai Nie Qiong Yang Lawrence G. Harshman Cungui Mao Nicholas A. Williamson Ary A. Hoffmann |
| author_facet | Meng-Jia Lau Shuai Nie Qiong Yang Lawrence G. Harshman Cungui Mao Nicholas A. Williamson Ary A. Hoffmann |
| author_sort | Meng-Jia Lau |
| collection | DOAJ |
| description | We conducted a lipidomic analysis of the whole body of female <i>Aedes aegypti</i> mosquitoes at different time points over the course of feeding and reproduction. There were temporal biphasic increases of more than 80% of lipids identified at the time of feeding and from 16 h to 30 h post blood meal (PBM). During these two increases, the abundance of many lipids dropped while body weight remained stable, probably reflecting blood lipid digestion and the synthesis of vitellogenin in this period. A concerted temporal pattern was particularly strong at the second peak for membrane and signalling lipids such as phosphatidylethanolamine (PE), phosphatidylinositol (PI), cardiolipin (CL), hexosylceramide (HexCer) and lyso-phosphatidic acid (LPA). Lyso-glycerophospholipids showed three distinct change patterns that are functionally related: Lyso-PE and Lyso-phosphatidylcholine (LPC), which are membrane lipids, showed little change; LPA, a signalling lipid, showed a significant increase from 16 to 30 h PBM; Lyso-PI, a bioactive lipid, and both lyso-phosphatidylglycerol (LPG) and lyso-phosphatidylserine (LPS), which are bacterial membrane lipids, showed one significant increase from the time of feeding to 16 h post blood meal. The result of our study on the anautogenous insect <i>Ae. aegypti</i> point to specific lipids likely to be important in the reproductive process with a role in the formation and growth of ovarian follicles. |
| first_indexed | 2024-03-11T06:11:48Z |
| format | Article |
| id | doaj.art-e72b4861d9a545a788bddd3321b89f9e |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T06:11:48Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-e72b4861d9a545a788bddd3321b89f9e2023-11-17T12:37:15ZengMDPI AGMetabolites2218-19892023-03-0113342110.3390/metabo13030421Lipidomic Profiling Reveals Concerted Temporal Patterns of Functionally Related Lipids in <i>Aedes aegypti</i> Females Following Blood FeedingMeng-Jia Lau0Shuai Nie1Qiong Yang2Lawrence G. Harshman3Cungui Mao4Nicholas A. Williamson5Ary A. Hoffmann6Pest and Environmental Adaptation Research Group, Bio21 Institute and the School of BioSciences, The University of Melbourne, Parkville, VIC 3052, AustraliaMass Spectrometry and Proteomics Facility, Bio21 Institute, The University of Melbourne, Parkville, VIC 3052, AustraliaPest and Environmental Adaptation Research Group, Bio21 Institute and the School of BioSciences, The University of Melbourne, Parkville, VIC 3052, AustraliaPest and Environmental Adaptation Research Group, Bio21 Institute and the School of BioSciences, The University of Melbourne, Parkville, VIC 3052, AustraliaDepartment of Medicine and Stony Brook Cancer Center, Renaissance School of Medicine, Stony Brook University, Stony Brook, New York, NY 11794, USAMass Spectrometry and Proteomics Facility, Bio21 Institute, The University of Melbourne, Parkville, VIC 3052, AustraliaPest and Environmental Adaptation Research Group, Bio21 Institute and the School of BioSciences, The University of Melbourne, Parkville, VIC 3052, AustraliaWe conducted a lipidomic analysis of the whole body of female <i>Aedes aegypti</i> mosquitoes at different time points over the course of feeding and reproduction. There were temporal biphasic increases of more than 80% of lipids identified at the time of feeding and from 16 h to 30 h post blood meal (PBM). During these two increases, the abundance of many lipids dropped while body weight remained stable, probably reflecting blood lipid digestion and the synthesis of vitellogenin in this period. A concerted temporal pattern was particularly strong at the second peak for membrane and signalling lipids such as phosphatidylethanolamine (PE), phosphatidylinositol (PI), cardiolipin (CL), hexosylceramide (HexCer) and lyso-phosphatidic acid (LPA). Lyso-glycerophospholipids showed three distinct change patterns that are functionally related: Lyso-PE and Lyso-phosphatidylcholine (LPC), which are membrane lipids, showed little change; LPA, a signalling lipid, showed a significant increase from 16 to 30 h PBM; Lyso-PI, a bioactive lipid, and both lyso-phosphatidylglycerol (LPG) and lyso-phosphatidylserine (LPS), which are bacterial membrane lipids, showed one significant increase from the time of feeding to 16 h post blood meal. The result of our study on the anautogenous insect <i>Ae. aegypti</i> point to specific lipids likely to be important in the reproductive process with a role in the formation and growth of ovarian follicles.https://www.mdpi.com/2218-1989/13/3/421lipidomics<i>Aedes aegypti</i>reproductionblood digestion |
| spellingShingle | Meng-Jia Lau Shuai Nie Qiong Yang Lawrence G. Harshman Cungui Mao Nicholas A. Williamson Ary A. Hoffmann Lipidomic Profiling Reveals Concerted Temporal Patterns of Functionally Related Lipids in <i>Aedes aegypti</i> Females Following Blood Feeding Metabolites lipidomics <i>Aedes aegypti</i> reproduction blood digestion |
| title | Lipidomic Profiling Reveals Concerted Temporal Patterns of Functionally Related Lipids in <i>Aedes aegypti</i> Females Following Blood Feeding |
| title_full | Lipidomic Profiling Reveals Concerted Temporal Patterns of Functionally Related Lipids in <i>Aedes aegypti</i> Females Following Blood Feeding |
| title_fullStr | Lipidomic Profiling Reveals Concerted Temporal Patterns of Functionally Related Lipids in <i>Aedes aegypti</i> Females Following Blood Feeding |
| title_full_unstemmed | Lipidomic Profiling Reveals Concerted Temporal Patterns of Functionally Related Lipids in <i>Aedes aegypti</i> Females Following Blood Feeding |
| title_short | Lipidomic Profiling Reveals Concerted Temporal Patterns of Functionally Related Lipids in <i>Aedes aegypti</i> Females Following Blood Feeding |
| title_sort | lipidomic profiling reveals concerted temporal patterns of functionally related lipids in i aedes aegypti i females following blood feeding |
| topic | lipidomics <i>Aedes aegypti</i> reproduction blood digestion |
| url | https://www.mdpi.com/2218-1989/13/3/421 |
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