Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions
In this work, a stability study of dispersions of graphene oxide and graphene oxide functionalized with polyethylene glycol (PEG) in the presence of bovine serum albumin is carried out. First, a structural characterization of these nanomaterials is performed by scanning electron microscopy, atomic f...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Journal of Xenobiotics |
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| Online Access: | https://www.mdpi.com/2039-4713/13/1/8 |
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| author | Javier Pérez-Piñeiro Fernando Sánchez-Cea Mariana P. Arce Isabel Lado-Touriño María Luisa Rojas-Cervantes María Fuencisla Gilsanz Darío Gallach-Pérez Rodrigo Blasco Niurka Barrios-Bermúdez Arisbel Cerpa-Naranjo |
| author_facet | Javier Pérez-Piñeiro Fernando Sánchez-Cea Mariana P. Arce Isabel Lado-Touriño María Luisa Rojas-Cervantes María Fuencisla Gilsanz Darío Gallach-Pérez Rodrigo Blasco Niurka Barrios-Bermúdez Arisbel Cerpa-Naranjo |
| author_sort | Javier Pérez-Piñeiro |
| collection | DOAJ |
| description | In this work, a stability study of dispersions of graphene oxide and graphene oxide functionalized with polyethylene glycol (PEG) in the presence of bovine serum albumin is carried out. First, a structural characterization of these nanomaterials is performed by scanning electron microscopy, atomic force microscopy, and ultraviolet visible spectroscopy, comparing the starting nanomaterials with the nanomaterials in contact with the biological material, i.e., bovine fetal serum. The different experiments were performed at different concentrations of nanomaterial (0.125–0.5 mg/mL) and BSA (0.01–0.04 mg/mL), at different incubation times (5–360 min), with and without PEG, and at different temperatures (25–40 °C). The SEM results show that BSA is adsorbed on the surface of the graphene oxide nanomaterial. Using UV-Vis spectrophotometry, the characteristic absorption peaks of BSA are observed at 210 and 280 nm, corroborating that the protein has been adsorbed. When the time increases, the BSA protein can be detached from the nanomaterial due to a desorption process. The stability of the dispersions is reached at a pH between 7 and 9. The dispersions behave like a Newtonian fluid with viscosity values between 1.1 and 1.5 mPa·s at a temperature range of 25 to 40 °C. The viscosity values decrease as the temperature increases. |
| first_indexed | 2024-03-11T06:20:36Z |
| format | Article |
| id | doaj.art-e7318210992d46ba8cce1609ede589d4 |
| institution | Directory Open Access Journal |
| issn | 2039-4705 2039-4713 |
| language | English |
| last_indexed | 2024-03-11T06:20:36Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Xenobiotics |
| spelling | doaj.art-e7318210992d46ba8cce1609ede589d42023-11-17T12:01:21ZengMDPI AGJournal of Xenobiotics2039-47052039-47132023-02-011319010110.3390/jox13010008Stability Study of Graphene Oxide-Bovine Serum Albumin DispersionsJavier Pérez-Piñeiro0Fernando Sánchez-Cea1Mariana P. Arce2Isabel Lado-Touriño3María Luisa Rojas-Cervantes4María Fuencisla Gilsanz5Darío Gallach-Pérez6Rodrigo Blasco7Niurka Barrios-Bermúdez8Arisbel Cerpa-Naranjo9School of Architecture, Engineering and Design, European University of Madrid, C/Tajo s/n Villaviciosa de Odón, 28670 Madrid, SpainSchool of Architecture, Engineering and Design, European University of Madrid, C/Tajo s/n Villaviciosa de Odón, 28670 Madrid, SpainSchool of Architecture, Engineering and Design, European University of Madrid, C/Tajo s/n Villaviciosa de Odón, 28670 Madrid, SpainSchool of Architecture, Engineering and Design, European University of Madrid, C/Tajo s/n Villaviciosa de Odón, 28670 Madrid, SpainDepartment of Inorganic and Technical Chemistry, Universidad Nacional de Educación a Distancia (UNED), Urbanización Monterrozas, Las Rozas, 28232 Madrid, SpainSchool of Architecture, Engineering and Design, European University of Madrid, C/Tajo s/n Villaviciosa de Odón, 28670 Madrid, SpainSchool of Architecture, Engineering and Design, European University of Madrid, C/Tajo s/n Villaviciosa de Odón, 28670 Madrid, SpainSchool of Architecture, Engineering and Design, European University of Madrid, C/Tajo s/n Villaviciosa de Odón, 28670 Madrid, SpainSchool of Architecture, Engineering and Design, European University of Madrid, C/Tajo s/n Villaviciosa de Odón, 28670 Madrid, SpainSchool of Architecture, Engineering and Design, European University of Madrid, C/Tajo s/n Villaviciosa de Odón, 28670 Madrid, SpainIn this work, a stability study of dispersions of graphene oxide and graphene oxide functionalized with polyethylene glycol (PEG) in the presence of bovine serum albumin is carried out. First, a structural characterization of these nanomaterials is performed by scanning electron microscopy, atomic force microscopy, and ultraviolet visible spectroscopy, comparing the starting nanomaterials with the nanomaterials in contact with the biological material, i.e., bovine fetal serum. The different experiments were performed at different concentrations of nanomaterial (0.125–0.5 mg/mL) and BSA (0.01–0.04 mg/mL), at different incubation times (5–360 min), with and without PEG, and at different temperatures (25–40 °C). The SEM results show that BSA is adsorbed on the surface of the graphene oxide nanomaterial. Using UV-Vis spectrophotometry, the characteristic absorption peaks of BSA are observed at 210 and 280 nm, corroborating that the protein has been adsorbed. When the time increases, the BSA protein can be detached from the nanomaterial due to a desorption process. The stability of the dispersions is reached at a pH between 7 and 9. The dispersions behave like a Newtonian fluid with viscosity values between 1.1 and 1.5 mPa·s at a temperature range of 25 to 40 °C. The viscosity values decrease as the temperature increases.https://www.mdpi.com/2039-4713/13/1/8graphene oxidebiologic fluidsbovine serum albumin |
| spellingShingle | Javier Pérez-Piñeiro Fernando Sánchez-Cea Mariana P. Arce Isabel Lado-Touriño María Luisa Rojas-Cervantes María Fuencisla Gilsanz Darío Gallach-Pérez Rodrigo Blasco Niurka Barrios-Bermúdez Arisbel Cerpa-Naranjo Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions Journal of Xenobiotics graphene oxide biologic fluids bovine serum albumin |
| title | Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions |
| title_full | Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions |
| title_fullStr | Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions |
| title_full_unstemmed | Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions |
| title_short | Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions |
| title_sort | stability study of graphene oxide bovine serum albumin dispersions |
| topic | graphene oxide biologic fluids bovine serum albumin |
| url | https://www.mdpi.com/2039-4713/13/1/8 |
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