Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the Environment
Pyridaben, a broad-spectrum pyridazinone acaricide that is widely used in agricultural production, can induce neurotoxicity and reproductive abnormalities, and is highly toxic to aquatic organisms. In this study, a pyridaben hapten was synthesized and used to prepare monoclonal antibodies (mAbs), am...
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MDPI AG
2023-05-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/5/545 |
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| author | He Chen Hao Liu Yanran Ji Zekun Sha Li An Meng Li Di Zhang Xujin Wu Xiude Hua |
| author_facet | He Chen Hao Liu Yanran Ji Zekun Sha Li An Meng Li Di Zhang Xujin Wu Xiude Hua |
| author_sort | He Chen |
| collection | DOAJ |
| description | Pyridaben, a broad-spectrum pyridazinone acaricide that is widely used in agricultural production, can induce neurotoxicity and reproductive abnormalities, and is highly toxic to aquatic organisms. In this study, a pyridaben hapten was synthesized and used to prepare monoclonal antibodies (mAbs), among which 6E3G8D7 showed the highest sensitivity in indirect competitive enzyme-linked immunosorbent assay, with a 50% inhibitory concentration (IC<sub>50</sub>) of 3.49 ng mL<sup>−1</sup>. The mAb, 6E3G8D7, was further applied to a gold nanoparticle-based colorimetric lateral flow immunoassay (CLFIA) for pyridaben detection, according to the signal intensity ratio of the test line to the control line, which showed a visual limit of detection of 5 ng mL<sup>−1</sup>. The CLFIA also showed high specificity and achieved excellent accuracy in different matrices. In addition, the amounts of pyridaben in blind samples detected by the CLFIA, were consistent with high-performance liquid chromatography. Therefore, the developed CLFIA is considered a promising, reliable, and portable method for pyridaben on-site detection in agro-products and environmental samples. |
| first_indexed | 2024-03-11T03:54:14Z |
| format | Article |
| id | doaj.art-e731840f76744a7d9c5174c505159656 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T03:54:14Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-e731840f76744a7d9c5174c5051596562023-11-18T00:41:14ZengMDPI AGBiosensors2079-63742023-05-0113554510.3390/bios13050545Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the EnvironmentHe Chen0Hao Liu1Yanran Ji2Zekun Sha3Li An4Meng Li5Di Zhang6Xujin Wu7Xiude Hua8Institute of Quality Standard and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaSchool of Life Sciences, Henan University, Kaifeng 475004, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaInstitute of Quality Standard and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaInstitute of Quality Standard and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaInstitute of Quality Standard and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaInstitute of Quality Standard and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou 450002, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaPyridaben, a broad-spectrum pyridazinone acaricide that is widely used in agricultural production, can induce neurotoxicity and reproductive abnormalities, and is highly toxic to aquatic organisms. In this study, a pyridaben hapten was synthesized and used to prepare monoclonal antibodies (mAbs), among which 6E3G8D7 showed the highest sensitivity in indirect competitive enzyme-linked immunosorbent assay, with a 50% inhibitory concentration (IC<sub>50</sub>) of 3.49 ng mL<sup>−1</sup>. The mAb, 6E3G8D7, was further applied to a gold nanoparticle-based colorimetric lateral flow immunoassay (CLFIA) for pyridaben detection, according to the signal intensity ratio of the test line to the control line, which showed a visual limit of detection of 5 ng mL<sup>−1</sup>. The CLFIA also showed high specificity and achieved excellent accuracy in different matrices. In addition, the amounts of pyridaben in blind samples detected by the CLFIA, were consistent with high-performance liquid chromatography. Therefore, the developed CLFIA is considered a promising, reliable, and portable method for pyridaben on-site detection in agro-products and environmental samples.https://www.mdpi.com/2079-6374/13/5/545pyridabenmonoclonal antibodycolorimetric lateral flow immunoassayenzyme-linked immunosorbent assayon-site detection |
| spellingShingle | He Chen Hao Liu Yanran Ji Zekun Sha Li An Meng Li Di Zhang Xujin Wu Xiude Hua Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the Environment Biosensors pyridaben monoclonal antibody colorimetric lateral flow immunoassay enzyme-linked immunosorbent assay on-site detection |
| title | Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the Environment |
| title_full | Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the Environment |
| title_fullStr | Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the Environment |
| title_full_unstemmed | Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the Environment |
| title_short | Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the Environment |
| title_sort | monoclonal antibody based colorimetric lateral flow immunoassay for the detection of pyridaben in the environment |
| topic | pyridaben monoclonal antibody colorimetric lateral flow immunoassay enzyme-linked immunosorbent assay on-site detection |
| url | https://www.mdpi.com/2079-6374/13/5/545 |
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