OsGLP participates in the regulation of lignin synthesis and deposition in rice against copper and cadmium toxicity
Copper (Cu) and cadmium (Cd) are common heavy metal pollutants. When Cd and excessive Cu accumulate in plants, plant growth is reduced. Our previous study showed that Germin-like proteins (GLPs), which exist in tandem on chromosomes, are a class of soluble glycoproteins that respond to Cu stress. In...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1078113/full |
| _version_ | 1797955318321250304 |
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| author | Xiangchao ShangGuan Ying Qi Aiguo Wang Yingnan Ren Yu Wang Tengwei Xiao Zhenguo Shen Qi Wang Yan Xia |
| author_facet | Xiangchao ShangGuan Ying Qi Aiguo Wang Yingnan Ren Yu Wang Tengwei Xiao Zhenguo Shen Qi Wang Yan Xia |
| author_sort | Xiangchao ShangGuan |
| collection | DOAJ |
| description | Copper (Cu) and cadmium (Cd) are common heavy metal pollutants. When Cd and excessive Cu accumulate in plants, plant growth is reduced. Our previous study showed that Germin-like proteins (GLPs), which exist in tandem on chromosomes, are a class of soluble glycoproteins that respond to Cu stress. In this study, hydroponic cultures were carried out to investigate the effect of GLP on Cd and Cu tolerance and accumulation in rice. The results showed that knockout of a single OsGLP8-2 gene or ten OsGLP genes (OsGLP8-2 to OsGLP8-11) resulted in a similar sensitivity to Cd and Cu toxicity. When subjected to Cu and Cd stress, the glp8-2 and glp8-(2-11) mutants displayed a more sensitive phenotype based on the plant height, root length, and dry biomass of the rice seedlings. Correspondingly, Cu and Cd concentrations in the glp8-2 and glp8-(2-11) mutants were significantly higher than those in the wild-type (WT) and OsGLP8-2-overexpressing line. However, Cu and Cd accumulation in the cell wall was the opposite. Furthermore, we determined lignin accumulation. The overexpressing-OsGLP8-2 line had a higher lignin accumulation in the shoot and root cell walls than those of the WT, glp8-2, and glp8-(2-11). The expression of lignin synthesis genes in the OsGLP8-2-overexpressing line was significantly higher than that in the WT, glp8-2, and glp8-(2-11). The SOD activity of OsGLP8-2, Diaminobe-nzidine (DAB), propidium iodide (PI) staining, and Malondialdehyde (MDA) content determination suggested that OsGLP8-2 is involved in heavy metal-induced antioxidant defense in rice. Our findings clearly suggest that OsGLPs participate in responses to heavy metal stress by lignin deposition and antioxidant defense capacity in rice, and OsGLP8-2 may play a major role in the tandem repeat gene clusters of chromosome 8 under heavy metal stress conditions. |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T23:32:18Z |
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| spelling | doaj.art-e733cdcb93f04b1c99943a66973cc9ad2023-01-12T06:10:23ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011310.3389/fpls.2022.10781131078113OsGLP participates in the regulation of lignin synthesis and deposition in rice against copper and cadmium toxicityXiangchao ShangGuan0Ying Qi1Aiguo Wang2Yingnan Ren3Yu Wang4Tengwei Xiao5Zhenguo Shen6Qi Wang7Yan Xia8College of Life Sciences, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, Nanjing Agricultural University, Nanjing, ChinaCollege of Agronomy, Yunnan Research Center of Urban Agricultural Engineering and Technology, Kunming University, Kunming, ChinaKey Laboratory of Ecological Environment and Tobacco Quality in Tobacco Industry, Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou, ChinaCollege of Life Sciences, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, Nanjing Agricultural University, Nanjing, ChinaCollege of Life Sciences, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, Nanjing Agricultural University, Nanjing, ChinaCollege of Life Sciences, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, Nanjing Agricultural University, Nanjing, ChinaCollege of Life Sciences, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, Nanjing Agricultural University, Nanjing, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaCollege of Life Sciences, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, Nanjing Agricultural University, Nanjing, ChinaCopper (Cu) and cadmium (Cd) are common heavy metal pollutants. When Cd and excessive Cu accumulate in plants, plant growth is reduced. Our previous study showed that Germin-like proteins (GLPs), which exist in tandem on chromosomes, are a class of soluble glycoproteins that respond to Cu stress. In this study, hydroponic cultures were carried out to investigate the effect of GLP on Cd and Cu tolerance and accumulation in rice. The results showed that knockout of a single OsGLP8-2 gene or ten OsGLP genes (OsGLP8-2 to OsGLP8-11) resulted in a similar sensitivity to Cd and Cu toxicity. When subjected to Cu and Cd stress, the glp8-2 and glp8-(2-11) mutants displayed a more sensitive phenotype based on the plant height, root length, and dry biomass of the rice seedlings. Correspondingly, Cu and Cd concentrations in the glp8-2 and glp8-(2-11) mutants were significantly higher than those in the wild-type (WT) and OsGLP8-2-overexpressing line. However, Cu and Cd accumulation in the cell wall was the opposite. Furthermore, we determined lignin accumulation. The overexpressing-OsGLP8-2 line had a higher lignin accumulation in the shoot and root cell walls than those of the WT, glp8-2, and glp8-(2-11). The expression of lignin synthesis genes in the OsGLP8-2-overexpressing line was significantly higher than that in the WT, glp8-2, and glp8-(2-11). The SOD activity of OsGLP8-2, Diaminobe-nzidine (DAB), propidium iodide (PI) staining, and Malondialdehyde (MDA) content determination suggested that OsGLP8-2 is involved in heavy metal-induced antioxidant defense in rice. Our findings clearly suggest that OsGLPs participate in responses to heavy metal stress by lignin deposition and antioxidant defense capacity in rice, and OsGLP8-2 may play a major role in the tandem repeat gene clusters of chromosome 8 under heavy metal stress conditions.https://www.frontiersin.org/articles/10.3389/fpls.2022.1078113/fullOryza sativa L.germin-like proteinsheavy metallignin accumulationdetoxification |
| spellingShingle | Xiangchao ShangGuan Ying Qi Aiguo Wang Yingnan Ren Yu Wang Tengwei Xiao Zhenguo Shen Qi Wang Yan Xia OsGLP participates in the regulation of lignin synthesis and deposition in rice against copper and cadmium toxicity Frontiers in Plant Science Oryza sativa L. germin-like proteins heavy metal lignin accumulation detoxification |
| title | OsGLP participates in the regulation of lignin synthesis and deposition in rice against copper and cadmium toxicity |
| title_full | OsGLP participates in the regulation of lignin synthesis and deposition in rice against copper and cadmium toxicity |
| title_fullStr | OsGLP participates in the regulation of lignin synthesis and deposition in rice against copper and cadmium toxicity |
| title_full_unstemmed | OsGLP participates in the regulation of lignin synthesis and deposition in rice against copper and cadmium toxicity |
| title_short | OsGLP participates in the regulation of lignin synthesis and deposition in rice against copper and cadmium toxicity |
| title_sort | osglp participates in the regulation of lignin synthesis and deposition in rice against copper and cadmium toxicity |
| topic | Oryza sativa L. germin-like proteins heavy metal lignin accumulation detoxification |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1078113/full |
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