The effects of combined free radical scavenger and sildenafil therapy on age-associated erectile dysfunction: An animal model
Introduction: Aging results in erectile dysfunction that is partially attributed to decreased nitric oxide (NO) and increased free radical generation. Vitamin E enhances endothelial cell function and acts as a free radical scavenger; however, its benefits on erectile function in the elderly are unkn...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | Urology Annals |
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| Online Access: | http://www.urologyannals.com/article.asp?issn=0974-7796;year=2014;volume=6;issue=4;spage=314;epage=320;aulast=Kovac |
| _version_ | 1818507185833902080 |
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| author | Jason R Kovac Ling DeYoung Kyle J Lehmann Eric Chung Gerald B Brock |
| author_facet | Jason R Kovac Ling DeYoung Kyle J Lehmann Eric Chung Gerald B Brock |
| author_sort | Jason R Kovac |
| collection | DOAJ |
| description | Introduction: Aging results in erectile dysfunction that is partially attributed to decreased nitric oxide (NO) and increased free radical generation. Vitamin E enhances endothelial cell function and acts as a free radical scavenger; however, its benefits on erectile function in the elderly are unknown.
Aims: The aim of the following study is to determine if Vitamin E alone, or in combination with the phosphodiesterase 5 inhibitor sildenafil, may improve erectile function and the NO signaling in a cohort of aged (13-15 month old) rats.
Materials and Methods: Male Sprague-Dawley rats (n = 28) were divided based upon age into young (4-5 months old, n = 7) and aged (13-15 months old, n = 21) cohorts. Aged rats were treated with Vitamin E, sildenafil or a combination of both. Penile cavernosal and dorsal nerve tissues were evaluated for neuronal nitric oxide synthase (nNOS) and caveolin-1 expression. Erectile function was assessed through intra-cavernous pressure (ICP) recordings.
Results: nNOS and cavoelin-1 were significantly decreased in aged rats compared with young controls. In aged rats, both Vitamin E and sildenafil partially recovered nNOS expression but when combined, a synergistic elevation in nNOS was observed. The significant decreases in ICP recorded in aged rats were improved with sildenafil; however, Vitamin E did not yield any additional improvements in ICP.
Conclusions: Diminished levels of nNOS and caveolin-1 are found in aged rats. When combined with sildenafil, Vitamin E synergistically increased nNOS expression. Since biochemical gains were not realized physiologically, other contributing factors likely exist. |
| first_indexed | 2024-12-10T22:15:04Z |
| format | Article |
| id | doaj.art-e7363701156e45c5a262eab4e78a00ea |
| institution | Directory Open Access Journal |
| issn | 0974-7796 0974-7834 |
| language | English |
| last_indexed | 2024-12-10T22:15:04Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Urology Annals |
| spelling | doaj.art-e7363701156e45c5a262eab4e78a00ea2022-12-22T01:31:30ZengWolters Kluwer Medknow PublicationsUrology Annals0974-77960974-78342014-01-016431432010.4103/0974-7796.140993The effects of combined free radical scavenger and sildenafil therapy on age-associated erectile dysfunction: An animal modelJason R KovacLing DeYoungKyle J LehmannEric ChungGerald B BrockIntroduction: Aging results in erectile dysfunction that is partially attributed to decreased nitric oxide (NO) and increased free radical generation. Vitamin E enhances endothelial cell function and acts as a free radical scavenger; however, its benefits on erectile function in the elderly are unknown. Aims: The aim of the following study is to determine if Vitamin E alone, or in combination with the phosphodiesterase 5 inhibitor sildenafil, may improve erectile function and the NO signaling in a cohort of aged (13-15 month old) rats. Materials and Methods: Male Sprague-Dawley rats (n = 28) were divided based upon age into young (4-5 months old, n = 7) and aged (13-15 months old, n = 21) cohorts. Aged rats were treated with Vitamin E, sildenafil or a combination of both. Penile cavernosal and dorsal nerve tissues were evaluated for neuronal nitric oxide synthase (nNOS) and caveolin-1 expression. Erectile function was assessed through intra-cavernous pressure (ICP) recordings. Results: nNOS and cavoelin-1 were significantly decreased in aged rats compared with young controls. In aged rats, both Vitamin E and sildenafil partially recovered nNOS expression but when combined, a synergistic elevation in nNOS was observed. The significant decreases in ICP recorded in aged rats were improved with sildenafil; however, Vitamin E did not yield any additional improvements in ICP. Conclusions: Diminished levels of nNOS and caveolin-1 are found in aged rats. When combined with sildenafil, Vitamin E synergistically increased nNOS expression. Since biochemical gains were not realized physiologically, other contributing factors likely exist.http://www.urologyannals.com/article.asp?issn=0974-7796;year=2014;volume=6;issue=4;spage=314;epage=320;aulast=KovacAgingantioxidanterectile dysfunctionimpotencenitric oxidesildenafilvitamin E |
| spellingShingle | Jason R Kovac Ling DeYoung Kyle J Lehmann Eric Chung Gerald B Brock The effects of combined free radical scavenger and sildenafil therapy on age-associated erectile dysfunction: An animal model Urology Annals Aging antioxidant erectile dysfunction impotence nitric oxide sildenafil vitamin E |
| title | The effects of combined free radical scavenger and sildenafil therapy on age-associated erectile dysfunction: An animal model |
| title_full | The effects of combined free radical scavenger and sildenafil therapy on age-associated erectile dysfunction: An animal model |
| title_fullStr | The effects of combined free radical scavenger and sildenafil therapy on age-associated erectile dysfunction: An animal model |
| title_full_unstemmed | The effects of combined free radical scavenger and sildenafil therapy on age-associated erectile dysfunction: An animal model |
| title_short | The effects of combined free radical scavenger and sildenafil therapy on age-associated erectile dysfunction: An animal model |
| title_sort | effects of combined free radical scavenger and sildenafil therapy on age associated erectile dysfunction an animal model |
| topic | Aging antioxidant erectile dysfunction impotence nitric oxide sildenafil vitamin E |
| url | http://www.urologyannals.com/article.asp?issn=0974-7796;year=2014;volume=6;issue=4;spage=314;epage=320;aulast=Kovac |
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