Preparation of amniotic membrane for ocular surface reconstruction
We describe the preparation and preservation of human amniotic membrane required for transplantation in the management of ocular surface diseases. Informed consent is obtained and the donor is screened to exclude risk of transmissible infections such as human immunodeficiency virus (HIV), hepatitis...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2002-01-01
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| Series: | Indian Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.in/article.asp?issn=0301-4738;year=2002;volume=50;issue=3;spage=227;epage=31;aulast=Madhavan |
| _version_ | 1819040353222656000 |
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| author | Madhavan Hajib Priya K Malathi J Joseph Patricia |
| author_facet | Madhavan Hajib Priya K Malathi J Joseph Patricia |
| author_sort | Madhavan Hajib |
| collection | DOAJ |
| description | We describe the preparation and preservation of human amniotic membrane required for transplantation in the management of ocular surface diseases. Informed consent is obtained and the donor is screened to exclude risk of transmissible infections such as human immunodeficiency virus (HIV), hepatitis B virus, hepatitis C virus, and <i>Treponema pallidum</i> infections. Ideally, the media and washing solutions needed for the preparation of amniotic membrane are prepared only a week to 10 days prior to use and not stored in the freezer weeks ahead. The AM obtained under sterile conditions after elective caesarian section is washed free of blood clots and chorion. With the epithelial surface up, amniotic membrane is spread uniformly without folds or tears on individually sterilized 0.22 &#956;m nitrocellulose membranes of the required sizes. The prepared filter membrane with the adherent amniotic membrane is placed in the preservative medium and stored at -80°C. The membranes are released when the repeat serology for HIV after the window period has excluded virus infection in the donor. Depending on consumption they may be used up to 6 months after preparation, though many have recommended storage for an indefinite period. Since the amniotic membrane has only incomplete expression of HLA antigens and amniotic epithelial cells do not express them, it is not rejected after transplantation. The presence of several cytokines in the amniotic membrane promotes epithelialization with reduction of fibrosis during healing. |
| first_indexed | 2024-12-21T09:07:45Z |
| format | Article |
| id | doaj.art-e73729b29e5c4f6e9f56235e00a8b839 |
| institution | Directory Open Access Journal |
| issn | 0301-4738 |
| language | English |
| last_indexed | 2024-12-21T09:07:45Z |
| publishDate | 2002-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Ophthalmology |
| spelling | doaj.art-e73729b29e5c4f6e9f56235e00a8b8392022-12-21T19:09:18ZengWolters Kluwer Medknow PublicationsIndian Journal of Ophthalmology0301-47382002-01-0150322731Preparation of amniotic membrane for ocular surface reconstructionMadhavan HajibPriya KMalathi JJoseph PatriciaWe describe the preparation and preservation of human amniotic membrane required for transplantation in the management of ocular surface diseases. Informed consent is obtained and the donor is screened to exclude risk of transmissible infections such as human immunodeficiency virus (HIV), hepatitis B virus, hepatitis C virus, and <i>Treponema pallidum</i> infections. Ideally, the media and washing solutions needed for the preparation of amniotic membrane are prepared only a week to 10 days prior to use and not stored in the freezer weeks ahead. The AM obtained under sterile conditions after elective caesarian section is washed free of blood clots and chorion. With the epithelial surface up, amniotic membrane is spread uniformly without folds or tears on individually sterilized 0.22 &#956;m nitrocellulose membranes of the required sizes. The prepared filter membrane with the adherent amniotic membrane is placed in the preservative medium and stored at -80°C. The membranes are released when the repeat serology for HIV after the window period has excluded virus infection in the donor. Depending on consumption they may be used up to 6 months after preparation, though many have recommended storage for an indefinite period. Since the amniotic membrane has only incomplete expression of HLA antigens and amniotic epithelial cells do not express them, it is not rejected after transplantation. The presence of several cytokines in the amniotic membrane promotes epithelialization with reduction of fibrosis during healing.http://www.ijo.in/article.asp?issn=0301-4738;year=2002;volume=50;issue=3;spage=227;epage=31;aulast=MadhavanAmniontransplantationBiological DressingsHumansIndiaOphthalmologic Surgical ProceduresmethodsPractice GuidelinesReconstructive Surgical Proceduresmethods |
| spellingShingle | Madhavan Hajib Priya K Malathi J Joseph Patricia Preparation of amniotic membrane for ocular surface reconstruction Indian Journal of Ophthalmology Amnion transplantation Biological Dressings Humans India Ophthalmologic Surgical Procedures methods Practice Guidelines Reconstructive Surgical Procedures methods |
| title | Preparation of amniotic membrane for ocular surface reconstruction |
| title_full | Preparation of amniotic membrane for ocular surface reconstruction |
| title_fullStr | Preparation of amniotic membrane for ocular surface reconstruction |
| title_full_unstemmed | Preparation of amniotic membrane for ocular surface reconstruction |
| title_short | Preparation of amniotic membrane for ocular surface reconstruction |
| title_sort | preparation of amniotic membrane for ocular surface reconstruction |
| topic | Amnion transplantation Biological Dressings Humans India Ophthalmologic Surgical Procedures methods Practice Guidelines Reconstructive Surgical Procedures methods |
| url | http://www.ijo.in/article.asp?issn=0301-4738;year=2002;volume=50;issue=3;spage=227;epage=31;aulast=Madhavan |
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