Cefotaxime-, Ciprofloxacin-, and Extensively Drug-Resistant <i>Escherichia coli</i> O157:H7 and O55:H7 in Camel Meat
The present study aimed to explore for the first time the occurrence and the antimicrobial resistance profiles of <i>E. coli</i> O157:H7 and O55:H7 isolates in camel meat in Egypt. Among the 110 camel meat samples examined using standardized microbiological techniques, 10 (9.1%) and 32 (...
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MDPI AG
2023-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/7/1443 |
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| author | Khalid Ibrahim Sallam Yasmine Abd-Elrazik Mona Talaat Raslan Kálmán Imre Adriana Morar Viorel Herman Hanan Ahmed Zaher |
| author_facet | Khalid Ibrahim Sallam Yasmine Abd-Elrazik Mona Talaat Raslan Kálmán Imre Adriana Morar Viorel Herman Hanan Ahmed Zaher |
| author_sort | Khalid Ibrahim Sallam |
| collection | DOAJ |
| description | The present study aimed to explore for the first time the occurrence and the antimicrobial resistance profiles of <i>E. coli</i> O157:H7 and O55:H7 isolates in camel meat in Egypt. Among the 110 camel meat samples examined using standardized microbiological techniques, 10 (9.1%) and 32 (29.1%) were positive for <i>E. coli</i> O157:H7 and <i>E. coli</i> O55:H7, respectively. In total, 24 isolates were verified as <i>E. coli</i> O157:H7, while 102 isolates were confirmed serologically as <i>E. coli</i> O55:H7. Multiplex PCR revealed the existence of <i>eaeA</i>, <i>stx1</i>, <i>stx2</i>, and EHEC-<i>hlyA</i> among <i>E. coli</i> O157:H7 and O55:H7 isolates (<i>n</i> = 126) at various percentages. According to their resistance against 14 antibiotics, 16.7% and 83.3% of O157:H7 isolates and 8.6% and 76.5% of O55:H7 isolates were classified into extensively drug-resistant and multi-drug-resistant, respectively, whereas 29.4% and 22.2% of <i>E. coli</i> isolates were resistant to cefotaxime and ciprofloxacin, respectively. The study results emphasize that camel meat may be a vehicle for multi- and extensively drug-resistant <i>E. coli</i> O157:H7 and O55:H7 strains, indicating a potential threat to public health. Further studies based on the molecular evidence of the antimicrobial resistance genes and enrolling a larger number of samples are recommended for a better understanding of the antimicrobial resistance phenomenon of camel-meat-originating pathogenic <i>E. coli</i> strains. |
| first_indexed | 2024-03-11T05:38:16Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T05:38:16Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj.art-e739411b6c104927bc1a83ce95c4b94d2023-11-17T16:41:21ZengMDPI AGFoods2304-81582023-03-01127144310.3390/foods12071443Cefotaxime-, Ciprofloxacin-, and Extensively Drug-Resistant <i>Escherichia coli</i> O157:H7 and O55:H7 in Camel MeatKhalid Ibrahim Sallam0Yasmine Abd-Elrazik1Mona Talaat Raslan2Kálmán Imre3Adriana Morar4Viorel Herman5Hanan Ahmed Zaher6Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Food Hygiene and Control, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptFood Hygiene Department, Animal Health Research Institute (AHRI), Dokki, Giza, Cairo 12618, EgyptDepartment of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine, University of Life Sciences “King Mihai I” from Timișoara, 300645 Timișoara, RomaniaDepartment of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine, University of Life Sciences “King Mihai I” from Timișoara, 300645 Timișoara, RomaniaDepartment of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences “King Mihai I” from Timişoara, 300645 Timișoara, RomaniaDepartment of Food Hygiene and Control, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptThe present study aimed to explore for the first time the occurrence and the antimicrobial resistance profiles of <i>E. coli</i> O157:H7 and O55:H7 isolates in camel meat in Egypt. Among the 110 camel meat samples examined using standardized microbiological techniques, 10 (9.1%) and 32 (29.1%) were positive for <i>E. coli</i> O157:H7 and <i>E. coli</i> O55:H7, respectively. In total, 24 isolates were verified as <i>E. coli</i> O157:H7, while 102 isolates were confirmed serologically as <i>E. coli</i> O55:H7. Multiplex PCR revealed the existence of <i>eaeA</i>, <i>stx1</i>, <i>stx2</i>, and EHEC-<i>hlyA</i> among <i>E. coli</i> O157:H7 and O55:H7 isolates (<i>n</i> = 126) at various percentages. According to their resistance against 14 antibiotics, 16.7% and 83.3% of O157:H7 isolates and 8.6% and 76.5% of O55:H7 isolates were classified into extensively drug-resistant and multi-drug-resistant, respectively, whereas 29.4% and 22.2% of <i>E. coli</i> isolates were resistant to cefotaxime and ciprofloxacin, respectively. The study results emphasize that camel meat may be a vehicle for multi- and extensively drug-resistant <i>E. coli</i> O157:H7 and O55:H7 strains, indicating a potential threat to public health. Further studies based on the molecular evidence of the antimicrobial resistance genes and enrolling a larger number of samples are recommended for a better understanding of the antimicrobial resistance phenomenon of camel-meat-originating pathogenic <i>E. coli</i> strains.https://www.mdpi.com/2304-8158/12/7/1443camel meatShiga toxinsvirulence genesantimicrobial resistancePCR |
| spellingShingle | Khalid Ibrahim Sallam Yasmine Abd-Elrazik Mona Talaat Raslan Kálmán Imre Adriana Morar Viorel Herman Hanan Ahmed Zaher Cefotaxime-, Ciprofloxacin-, and Extensively Drug-Resistant <i>Escherichia coli</i> O157:H7 and O55:H7 in Camel Meat Foods camel meat Shiga toxins virulence genes antimicrobial resistance PCR |
| title | Cefotaxime-, Ciprofloxacin-, and Extensively Drug-Resistant <i>Escherichia coli</i> O157:H7 and O55:H7 in Camel Meat |
| title_full | Cefotaxime-, Ciprofloxacin-, and Extensively Drug-Resistant <i>Escherichia coli</i> O157:H7 and O55:H7 in Camel Meat |
| title_fullStr | Cefotaxime-, Ciprofloxacin-, and Extensively Drug-Resistant <i>Escherichia coli</i> O157:H7 and O55:H7 in Camel Meat |
| title_full_unstemmed | Cefotaxime-, Ciprofloxacin-, and Extensively Drug-Resistant <i>Escherichia coli</i> O157:H7 and O55:H7 in Camel Meat |
| title_short | Cefotaxime-, Ciprofloxacin-, and Extensively Drug-Resistant <i>Escherichia coli</i> O157:H7 and O55:H7 in Camel Meat |
| title_sort | cefotaxime ciprofloxacin and extensively drug resistant i escherichia coli i o157 h7 and o55 h7 in camel meat |
| topic | camel meat Shiga toxins virulence genes antimicrobial resistance PCR |
| url | https://www.mdpi.com/2304-8158/12/7/1443 |
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