Effects of Strain Magnitude on Mechanical Responses of Three-Dimensional Gel-Embedded Osteocytes Studied with a Novel 10-Well Elastic Chamber
Osteocytes inside bone matrix play an important role in detecting the local mechanical environment. In order to study their mechanical responses, we have developed a mechanical loading device that can apply physiological and supraphysiological strains to osteocytes embedded in a three dimensional (3...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The Japan Society of Mechanical Engineers
2008-02-01
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| Series: | Journal of Biomechanical Science and Engineering |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jbse/3/1/3_1_13/_pdf/-char/en |
| _version_ | 1811292447744983040 |
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| author | Takanobu FUKUNAGA Kosaku KURATA Junpei MATSUDA Hidehiko HIGAKI |
| author_facet | Takanobu FUKUNAGA Kosaku KURATA Junpei MATSUDA Hidehiko HIGAKI |
| author_sort | Takanobu FUKUNAGA |
| collection | DOAJ |
| description | Osteocytes inside bone matrix play an important role in detecting the local mechanical environment. In order to study their mechanical responses, we have developed a mechanical loading device that can apply physiological and supraphysiological strains to osteocytes embedded in a three dimensional (3-D) gel. The newly designed elastic chamber with ten separated culture wells can simultaneously apply five different strain magnitudes for the mechanical stretching of the cells. When the gel-embedded MLO-Y4 cells were prepared in the wells, they were subjected to mechanical stretching of physiological and supraphysiological strain levels for 24 h. The cell viability assay indicated that significant dead cells were observed for strain values greater than 8890 με. Beyond this threshold, the number of dead cells linearly increased with the strain magnitude. The supernatant of MLO-Y4 cells, which was exposed to strain levels beyond the threshold, showed a significant increase in tartrate-resistant acid phosphatase (TRACP) activity in the bone marrow culture. These experimental findings indicate that the local death of osteocytes provides an important mechanism to initiate bone resorption. |
| first_indexed | 2024-04-13T04:45:33Z |
| format | Article |
| id | doaj.art-e73a8a27d70b47198ca74bb4fa0ad09e |
| institution | Directory Open Access Journal |
| issn | 1880-9863 |
| language | English |
| last_indexed | 2024-04-13T04:45:33Z |
| publishDate | 2008-02-01 |
| publisher | The Japan Society of Mechanical Engineers |
| record_format | Article |
| series | Journal of Biomechanical Science and Engineering |
| spelling | doaj.art-e73a8a27d70b47198ca74bb4fa0ad09e2022-12-22T03:01:52ZengThe Japan Society of Mechanical EngineersJournal of Biomechanical Science and Engineering1880-98632008-02-0131132410.1299/jbse.3.13jbseEffects of Strain Magnitude on Mechanical Responses of Three-Dimensional Gel-Embedded Osteocytes Studied with a Novel 10-Well Elastic ChamberTakanobu FUKUNAGA0Kosaku KURATA1Junpei MATSUDA2Hidehiko HIGAKI3Graduate School of Engineering, Kyushu Sangyo UniversityDepartment of Biorobotics, Faculty of Engineering, Kyushu Sangyo UniversityGraduate School of Engineering, Kyushu Sangyo UniversityDepartment of Biorobotics, Faculty of Engineering, Kyushu Sangyo UniversityOsteocytes inside bone matrix play an important role in detecting the local mechanical environment. In order to study their mechanical responses, we have developed a mechanical loading device that can apply physiological and supraphysiological strains to osteocytes embedded in a three dimensional (3-D) gel. The newly designed elastic chamber with ten separated culture wells can simultaneously apply five different strain magnitudes for the mechanical stretching of the cells. When the gel-embedded MLO-Y4 cells were prepared in the wells, they were subjected to mechanical stretching of physiological and supraphysiological strain levels for 24 h. The cell viability assay indicated that significant dead cells were observed for strain values greater than 8890 με. Beyond this threshold, the number of dead cells linearly increased with the strain magnitude. The supernatant of MLO-Y4 cells, which was exposed to strain levels beyond the threshold, showed a significant increase in tartrate-resistant acid phosphatase (TRACP) activity in the bone marrow culture. These experimental findings indicate that the local death of osteocytes provides an important mechanism to initiate bone resorption.https://www.jstage.jst.go.jp/article/jbse/3/1/3_1_13/_pdf/-char/enosteocytemechanical stretchingcell deathbone remodelingcell differentiation |
| spellingShingle | Takanobu FUKUNAGA Kosaku KURATA Junpei MATSUDA Hidehiko HIGAKI Effects of Strain Magnitude on Mechanical Responses of Three-Dimensional Gel-Embedded Osteocytes Studied with a Novel 10-Well Elastic Chamber Journal of Biomechanical Science and Engineering osteocyte mechanical stretching cell death bone remodeling cell differentiation |
| title | Effects of Strain Magnitude on Mechanical Responses of Three-Dimensional Gel-Embedded Osteocytes Studied with a Novel 10-Well Elastic Chamber |
| title_full | Effects of Strain Magnitude on Mechanical Responses of Three-Dimensional Gel-Embedded Osteocytes Studied with a Novel 10-Well Elastic Chamber |
| title_fullStr | Effects of Strain Magnitude on Mechanical Responses of Three-Dimensional Gel-Embedded Osteocytes Studied with a Novel 10-Well Elastic Chamber |
| title_full_unstemmed | Effects of Strain Magnitude on Mechanical Responses of Three-Dimensional Gel-Embedded Osteocytes Studied with a Novel 10-Well Elastic Chamber |
| title_short | Effects of Strain Magnitude on Mechanical Responses of Three-Dimensional Gel-Embedded Osteocytes Studied with a Novel 10-Well Elastic Chamber |
| title_sort | effects of strain magnitude on mechanical responses of three dimensional gel embedded osteocytes studied with a novel 10 well elastic chamber |
| topic | osteocyte mechanical stretching cell death bone remodeling cell differentiation |
| url | https://www.jstage.jst.go.jp/article/jbse/3/1/3_1_13/_pdf/-char/en |
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