A Bovine Lymphosarcoma Cell Line Infected with Theileria annulata Exhibits an Irreversible Reconfiguration of Host Cell Gene Expression.
Theileria annulata, an intracellular parasite of bovine lymphoid cells, induces substantial phenotypic alterations to its host cell including continuous proliferation, cytoskeletal changes and resistance to apoptosis. While parasite induced modulation of host cell signal transduction pathways and NF...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3694138?pdf=render |
| _version_ | 1818616752982982656 |
|---|---|
| author | Jane H Kinnaird William Weir Zeeshan Durrani Sreerekha S Pillai Margaret Baird Brian R Shiels |
| author_facet | Jane H Kinnaird William Weir Zeeshan Durrani Sreerekha S Pillai Margaret Baird Brian R Shiels |
| author_sort | Jane H Kinnaird |
| collection | DOAJ |
| description | Theileria annulata, an intracellular parasite of bovine lymphoid cells, induces substantial phenotypic alterations to its host cell including continuous proliferation, cytoskeletal changes and resistance to apoptosis. While parasite induced modulation of host cell signal transduction pathways and NFκB activation are established, there remains considerable speculation on the complexities of the parasite directed control mechanisms that govern these radical changes to the host cell. Our objectives in this study were to provide a comprehensive analysis of the global changes to host cell gene expression with emphasis on those that result from direct intervention by the parasite. By using comparative microarray analysis of an uninfected bovine cell line and its Theileria infected counterpart, in conjunction with use of the specific parasitacidal agent, buparvaquone, we have identified a large number of host cell gene expression changes that result from parasite infection. Our results indicate that the viable parasite can irreversibly modify the transformed phenotype of a bovine cell line. Fifty percent of genes with altered expression failed to show a reversible response to parasite death, a possible contributing factor to initiation of host cell apoptosis. The genes that did show an early predicted response to loss of parasite viability highlighted a sub-group of genes that are likely to be under direct control by parasite infection. Network and pathway analysis demonstrated that this sub-group is significantly enriched for genes involved in regulation of chromatin modification and gene expression. The results provide evidence that the Theileria parasite has the regulatory capacity to generate widespread change to host cell gene expression in a complex and largely irreversible manner. |
| first_indexed | 2024-12-16T16:54:48Z |
| format | Article |
| id | doaj.art-e73bf058d39e4668a71297724a84b083 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T16:54:48Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e73bf058d39e4668a71297724a84b0832022-12-21T22:23:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6683310.1371/journal.pone.0066833A Bovine Lymphosarcoma Cell Line Infected with Theileria annulata Exhibits an Irreversible Reconfiguration of Host Cell Gene Expression.Jane H KinnairdWilliam WeirZeeshan DurraniSreerekha S PillaiMargaret BairdBrian R ShielsTheileria annulata, an intracellular parasite of bovine lymphoid cells, induces substantial phenotypic alterations to its host cell including continuous proliferation, cytoskeletal changes and resistance to apoptosis. While parasite induced modulation of host cell signal transduction pathways and NFκB activation are established, there remains considerable speculation on the complexities of the parasite directed control mechanisms that govern these radical changes to the host cell. Our objectives in this study were to provide a comprehensive analysis of the global changes to host cell gene expression with emphasis on those that result from direct intervention by the parasite. By using comparative microarray analysis of an uninfected bovine cell line and its Theileria infected counterpart, in conjunction with use of the specific parasitacidal agent, buparvaquone, we have identified a large number of host cell gene expression changes that result from parasite infection. Our results indicate that the viable parasite can irreversibly modify the transformed phenotype of a bovine cell line. Fifty percent of genes with altered expression failed to show a reversible response to parasite death, a possible contributing factor to initiation of host cell apoptosis. The genes that did show an early predicted response to loss of parasite viability highlighted a sub-group of genes that are likely to be under direct control by parasite infection. Network and pathway analysis demonstrated that this sub-group is significantly enriched for genes involved in regulation of chromatin modification and gene expression. The results provide evidence that the Theileria parasite has the regulatory capacity to generate widespread change to host cell gene expression in a complex and largely irreversible manner.http://europepmc.org/articles/PMC3694138?pdf=render |
| spellingShingle | Jane H Kinnaird William Weir Zeeshan Durrani Sreerekha S Pillai Margaret Baird Brian R Shiels A Bovine Lymphosarcoma Cell Line Infected with Theileria annulata Exhibits an Irreversible Reconfiguration of Host Cell Gene Expression. PLoS ONE |
| title | A Bovine Lymphosarcoma Cell Line Infected with Theileria annulata Exhibits an Irreversible Reconfiguration of Host Cell Gene Expression. |
| title_full | A Bovine Lymphosarcoma Cell Line Infected with Theileria annulata Exhibits an Irreversible Reconfiguration of Host Cell Gene Expression. |
| title_fullStr | A Bovine Lymphosarcoma Cell Line Infected with Theileria annulata Exhibits an Irreversible Reconfiguration of Host Cell Gene Expression. |
| title_full_unstemmed | A Bovine Lymphosarcoma Cell Line Infected with Theileria annulata Exhibits an Irreversible Reconfiguration of Host Cell Gene Expression. |
| title_short | A Bovine Lymphosarcoma Cell Line Infected with Theileria annulata Exhibits an Irreversible Reconfiguration of Host Cell Gene Expression. |
| title_sort | bovine lymphosarcoma cell line infected with theileria annulata exhibits an irreversible reconfiguration of host cell gene expression |
| url | http://europepmc.org/articles/PMC3694138?pdf=render |
| work_keys_str_mv | AT janehkinnaird abovinelymphosarcomacelllineinfectedwiththeileriaannulataexhibitsanirreversiblereconfigurationofhostcellgeneexpression AT williamweir abovinelymphosarcomacelllineinfectedwiththeileriaannulataexhibitsanirreversiblereconfigurationofhostcellgeneexpression AT zeeshandurrani abovinelymphosarcomacelllineinfectedwiththeileriaannulataexhibitsanirreversiblereconfigurationofhostcellgeneexpression AT sreerekhaspillai abovinelymphosarcomacelllineinfectedwiththeileriaannulataexhibitsanirreversiblereconfigurationofhostcellgeneexpression AT margaretbaird abovinelymphosarcomacelllineinfectedwiththeileriaannulataexhibitsanirreversiblereconfigurationofhostcellgeneexpression AT brianrshiels abovinelymphosarcomacelllineinfectedwiththeileriaannulataexhibitsanirreversiblereconfigurationofhostcellgeneexpression AT janehkinnaird bovinelymphosarcomacelllineinfectedwiththeileriaannulataexhibitsanirreversiblereconfigurationofhostcellgeneexpression AT williamweir bovinelymphosarcomacelllineinfectedwiththeileriaannulataexhibitsanirreversiblereconfigurationofhostcellgeneexpression AT zeeshandurrani bovinelymphosarcomacelllineinfectedwiththeileriaannulataexhibitsanirreversiblereconfigurationofhostcellgeneexpression AT sreerekhaspillai bovinelymphosarcomacelllineinfectedwiththeileriaannulataexhibitsanirreversiblereconfigurationofhostcellgeneexpression AT margaretbaird bovinelymphosarcomacelllineinfectedwiththeileriaannulataexhibitsanirreversiblereconfigurationofhostcellgeneexpression AT brianrshiels bovinelymphosarcomacelllineinfectedwiththeileriaannulataexhibitsanirreversiblereconfigurationofhostcellgeneexpression |