Analysis ulcerative colitis for presence Epstein-Barr virus DNA sequences by polymerase chain reaction technique
Introduction: Ulcerative colitis (UC) is one type of inflammatory bowel disease (IBD). The purpose of this study is to explore the prevalence of Epstein–Barr virus (EBV) in UC patients in comparison with healthy subjects using the polymerase chain reaction (PCR) method. Methods: In...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2016-02-01
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| Series: | Journal of Analytical Research in Clinical Medicine |
| Subjects: | |
| Online Access: | http://journals.tbzmed.ac.ir/JARCM/Manuscript/JARCM-4-9.pdf |
| _version_ | 1819084778773676032 |
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| author | Sahar Mehrabani khasraghi Mitra Ameli Farzad Khalily |
| author_facet | Sahar Mehrabani khasraghi Mitra Ameli Farzad Khalily |
| author_sort | Sahar Mehrabani khasraghi |
| collection | DOAJ |
| description | Introduction: Ulcerative colitis (UC) is one type of inflammatory bowel
disease (IBD). The purpose of this study is to explore the prevalence of Epstein–Barr virus
(EBV) in UC patients in comparison with healthy subjects using the polymerase chain reaction
(PCR) method. Methods: In this case-control study, five biopsies of patients with UC and 30
healthy people as controls were selected. Sampling was performed by endoscopic biopsy
operation. After DNA extraction, PCR was used to determine EBV genome by specific primers.
Statistical analysis was performed using the chi-square test. Results: The results of PCR
indicated that EBV genome was detected in 60.0% of samples in the case group, and 36.7% of
samples in the control group were positive for EBV. Thus, no significant association was
observed between the prevalence of EBV and incidence of UC in comparison with the control
group (P = 0.36). Conclusion: The findings presented herein demonstrate no direct molecular
evidence to support an association of EBV with UC. These results, do not exclude the
possibility oncogenic role of EBV to infect the different colon cell. |
| first_indexed | 2024-12-21T20:53:52Z |
| format | Article |
| id | doaj.art-e73c5a56f9d245e687c54236da74e9f6 |
| institution | Directory Open Access Journal |
| issn | 2345-4970 |
| language | English |
| last_indexed | 2024-12-21T20:53:52Z |
| publishDate | 2016-02-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Journal of Analytical Research in Clinical Medicine |
| spelling | doaj.art-e73c5a56f9d245e687c54236da74e9f62022-12-21T18:50:39ZengTabriz University of Medical SciencesJournal of Analytical Research in Clinical Medicine2345-49702016-02-01419131015171/jarcm.2016.002JARCM_3382_20150831132108Analysis ulcerative colitis for presence Epstein-Barr virus DNA sequences by polymerase chain reaction techniqueSahar Mehrabani khasraghi0Mitra Ameli1Farzad Khalily2Microbiologist, Department of Microbiology, School of Basic Sciences, Islamic Azad University, Tonekabon Branch, Tonekabon, IranAssistant Professor, Liver and Gastrointestinal Disease Research Center, Tehran University of Medical Sciences, Tehran, IranAssistant Professor, Liver and Gastrointestinal Disease Research Center, Alborz University of Medical Sciences, Karaj, IranIntroduction: Ulcerative colitis (UC) is one type of inflammatory bowel disease (IBD). The purpose of this study is to explore the prevalence of Epstein–Barr virus (EBV) in UC patients in comparison with healthy subjects using the polymerase chain reaction (PCR) method. Methods: In this case-control study, five biopsies of patients with UC and 30 healthy people as controls were selected. Sampling was performed by endoscopic biopsy operation. After DNA extraction, PCR was used to determine EBV genome by specific primers. Statistical analysis was performed using the chi-square test. Results: The results of PCR indicated that EBV genome was detected in 60.0% of samples in the case group, and 36.7% of samples in the control group were positive for EBV. Thus, no significant association was observed between the prevalence of EBV and incidence of UC in comparison with the control group (P = 0.36). Conclusion: The findings presented herein demonstrate no direct molecular evidence to support an association of EBV with UC. These results, do not exclude the possibility oncogenic role of EBV to infect the different colon cell.http://journals.tbzmed.ac.ir/JARCM/Manuscript/JARCM-4-9.pdfUlcerative colitisEpstein-Barr virusPolymerase Chain Reaction |
| spellingShingle | Sahar Mehrabani khasraghi Mitra Ameli Farzad Khalily Analysis ulcerative colitis for presence Epstein-Barr virus DNA sequences by polymerase chain reaction technique Journal of Analytical Research in Clinical Medicine Ulcerative colitis Epstein-Barr virus Polymerase Chain Reaction |
| title | Analysis ulcerative colitis for presence Epstein-Barr virus DNA sequences
by polymerase chain reaction technique |
| title_full | Analysis ulcerative colitis for presence Epstein-Barr virus DNA sequences
by polymerase chain reaction technique |
| title_fullStr | Analysis ulcerative colitis for presence Epstein-Barr virus DNA sequences
by polymerase chain reaction technique |
| title_full_unstemmed | Analysis ulcerative colitis for presence Epstein-Barr virus DNA sequences
by polymerase chain reaction technique |
| title_short | Analysis ulcerative colitis for presence Epstein-Barr virus DNA sequences
by polymerase chain reaction technique |
| title_sort | analysis ulcerative colitis for presence epstein barr virus dna sequences by polymerase chain reaction technique |
| topic | Ulcerative colitis Epstein-Barr virus Polymerase Chain Reaction |
| url | http://journals.tbzmed.ac.ir/JARCM/Manuscript/JARCM-4-9.pdf |
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