Comparisons among rainbow trout, Oncorhynchus mykiss, populations of maternal transcript profile associated with egg viability
Abstract Background Transcription is arrested in the late stage oocyte and therefore the maternal transcriptome stored in the oocyte provides nearly all the mRNA required for oocyte maturation, fertilization, and early cleavage of the embryo. The transcriptome of the unfertilized egg, therefore, has...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-07773-1 |
| _version_ | 1818669143870668800 |
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| author | Gregory M. Weber Jill Birkett Kyle Martin Doug Dixon Guangtu Gao Timothy D. Leeds Roger L. Vallejo Hao Ma |
| author_facet | Gregory M. Weber Jill Birkett Kyle Martin Doug Dixon Guangtu Gao Timothy D. Leeds Roger L. Vallejo Hao Ma |
| author_sort | Gregory M. Weber |
| collection | DOAJ |
| description | Abstract Background Transcription is arrested in the late stage oocyte and therefore the maternal transcriptome stored in the oocyte provides nearly all the mRNA required for oocyte maturation, fertilization, and early cleavage of the embryo. The transcriptome of the unfertilized egg, therefore, has potential to provide markers for predictors of egg quality and diagnosing problems with embryo production encountered by fish hatcheries. Although levels of specific transcripts have been shown to associate with measures of egg quality, these differentially expressed genes (DEGs) have not been consistent among studies. The present study compares differences in select transcripts among unfertilized rainbow trout eggs of different quality based on eyeing rate, among 2 year classes of the same line (A1, A2) and a population from a different hatchery (B). The study compared 65 transcripts previously reported to be differentially expressed with egg quality in rainbow trout. Results There were 32 transcripts identified as DEGs among the three groups by regression analysis. Group A1 had the most DEGs, 26; A2 had 15, 14 of which were shared with A1; and B had 12, 7 of which overlapped with A1 or A2. Six transcripts were found in all three groups, dcaf11, impa2, mrpl39_like, senp7, tfip11 and uchl1. Conclusions Our results confirmed maternal transcripts found to be differentially expressed between low- and high-quality eggs in one population of rainbow trout can often be found to overlap with DEGs in other populations. The transcripts differentially expressed with egg quality remain consistent among year classes of the same line. Greater similarity in dysregulated transcripts within year classes of the same line than among lines suggests patterns of transcriptome dysregulation may provide insight into causes of decreased viability within a hatchery population. Although many DEGs were identified, for each of the genes there is considerable variability in transcript abundance among eggs of similar quality and low correlations between transcript abundance and eyeing rate, making it highly improbable to predict the quality of a single batch of eggs based on transcript abundance of just a few genes. |
| first_indexed | 2024-12-17T06:47:32Z |
| format | Article |
| id | doaj.art-e73f10d82464489085be18520c7d1296 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-17T06:47:32Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-e73f10d82464489085be18520c7d12962022-12-21T21:59:42ZengBMCBMC Genomics1471-21642021-06-0122111810.1186/s12864-021-07773-1Comparisons among rainbow trout, Oncorhynchus mykiss, populations of maternal transcript profile associated with egg viabilityGregory M. Weber0Jill Birkett1Kyle Martin2Doug Dixon3Guangtu Gao4Timothy D. Leeds5Roger L. Vallejo6Hao Ma7USDA/ARS National Center for Cool and Cold Water AquacultureUSDA/ARS National Center for Cool and Cold Water AquacultureTroutlodge Inc.Troutlodge Inc.USDA/ARS National Center for Cool and Cold Water AquacultureUSDA/ARS National Center for Cool and Cold Water AquacultureUSDA/ARS National Center for Cool and Cold Water AquacultureUSDA/ARS Ruminant Diseases and Immunology Research Unit, National Animal Disease CenterAbstract Background Transcription is arrested in the late stage oocyte and therefore the maternal transcriptome stored in the oocyte provides nearly all the mRNA required for oocyte maturation, fertilization, and early cleavage of the embryo. The transcriptome of the unfertilized egg, therefore, has potential to provide markers for predictors of egg quality and diagnosing problems with embryo production encountered by fish hatcheries. Although levels of specific transcripts have been shown to associate with measures of egg quality, these differentially expressed genes (DEGs) have not been consistent among studies. The present study compares differences in select transcripts among unfertilized rainbow trout eggs of different quality based on eyeing rate, among 2 year classes of the same line (A1, A2) and a population from a different hatchery (B). The study compared 65 transcripts previously reported to be differentially expressed with egg quality in rainbow trout. Results There were 32 transcripts identified as DEGs among the three groups by regression analysis. Group A1 had the most DEGs, 26; A2 had 15, 14 of which were shared with A1; and B had 12, 7 of which overlapped with A1 or A2. Six transcripts were found in all three groups, dcaf11, impa2, mrpl39_like, senp7, tfip11 and uchl1. Conclusions Our results confirmed maternal transcripts found to be differentially expressed between low- and high-quality eggs in one population of rainbow trout can often be found to overlap with DEGs in other populations. The transcripts differentially expressed with egg quality remain consistent among year classes of the same line. Greater similarity in dysregulated transcripts within year classes of the same line than among lines suggests patterns of transcriptome dysregulation may provide insight into causes of decreased viability within a hatchery population. Although many DEGs were identified, for each of the genes there is considerable variability in transcript abundance among eggs of similar quality and low correlations between transcript abundance and eyeing rate, making it highly improbable to predict the quality of a single batch of eggs based on transcript abundance of just a few genes.https://doi.org/10.1186/s12864-021-07773-1Rainbow troutEgg qualitymRNAMaternal RNAMitochondria |
| spellingShingle | Gregory M. Weber Jill Birkett Kyle Martin Doug Dixon Guangtu Gao Timothy D. Leeds Roger L. Vallejo Hao Ma Comparisons among rainbow trout, Oncorhynchus mykiss, populations of maternal transcript profile associated with egg viability BMC Genomics Rainbow trout Egg quality mRNA Maternal RNA Mitochondria |
| title | Comparisons among rainbow trout, Oncorhynchus mykiss, populations of maternal transcript profile associated with egg viability |
| title_full | Comparisons among rainbow trout, Oncorhynchus mykiss, populations of maternal transcript profile associated with egg viability |
| title_fullStr | Comparisons among rainbow trout, Oncorhynchus mykiss, populations of maternal transcript profile associated with egg viability |
| title_full_unstemmed | Comparisons among rainbow trout, Oncorhynchus mykiss, populations of maternal transcript profile associated with egg viability |
| title_short | Comparisons among rainbow trout, Oncorhynchus mykiss, populations of maternal transcript profile associated with egg viability |
| title_sort | comparisons among rainbow trout oncorhynchus mykiss populations of maternal transcript profile associated with egg viability |
| topic | Rainbow trout Egg quality mRNA Maternal RNA Mitochondria |
| url | https://doi.org/10.1186/s12864-021-07773-1 |
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