The Influence of Spirulina platensis Filtrates on Caco-2 Proliferative Activity and Expression of Apoptosis-Related microRNAs and mRNA
Spirulina platensis (SP) is a blue-green microalga that has recently raised attention not only as a nutritional component, but also as a source of bioactivities that have therapeutic effects and may find application in medicine, including cancer treatment. In the present study we determined the cyto...
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| Format: | Article |
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MDPI AG
2017-03-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/15/3/65 |
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| author | Agnieszka Śmieszek Ewa Giezek Martyna Chrapiec Martyna Murat Aleksandra Mucha Izabela Michalak Krzysztof Marycz |
| author_facet | Agnieszka Śmieszek Ewa Giezek Martyna Chrapiec Martyna Murat Aleksandra Mucha Izabela Michalak Krzysztof Marycz |
| author_sort | Agnieszka Śmieszek |
| collection | DOAJ |
| description | Spirulina platensis (SP) is a blue-green microalga that has recently raised attention not only as a nutritional component, but also as a source of bioactivities that have therapeutic effects and may find application in medicine, including cancer treatment. In the present study we determined the cytotoxic effect of S. platensis filtrates (SPF) on human colon cancer cell line Caco-2. Three concentrations of SPF were tested—1.25%, 2.5%, and 5% (v/v). We have found that the highest concentration of SPF exerts the strongest anti-proliferative and pro-apoptotic effect on Caco-2 cultures. The SPF negatively affected the morphology of Caco-2 causing colony shrinking and significant inhibition of metabolic and proliferative activity of cells. The wound-healing assay showed that the SPF impaired migratory capabilities of Caco-2. This observation was consistent with lowered mRNA levels for metalloproteinases. Furthermore, SPF decreased the transcript level of pro-survival genes (cyclin D1, surviving, and c-Myc) and reduced the autocrine secretion of Wnt-10b. The cytotoxic effect of SPF involved the modulation of the Bax and Bcl-2 ratio and a decrease of mitochondrial activity, and was related with increased levels of intracellular reactive oxygen species (ROS) and nitric oxide (NO). Moreover, the SPF also caused an increased number of cells in the apoptotic sub-G0 phase and up-regulated expression of mir-145, simultaneously decreasing expression of mir-17 and 146. Obtained results indicate that SPF can be considered as an agent with anti-cancer properties that may be used for colon cancer prevention and treatment. |
| first_indexed | 2024-04-13T06:56:25Z |
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| id | doaj.art-e73f32f7158c406c93fcb22194b2e6e3 |
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| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-13T06:56:25Z |
| publishDate | 2017-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-e73f32f7158c406c93fcb22194b2e6e32022-12-22T02:57:15ZengMDPI AGMarine Drugs1660-33972017-03-011536510.3390/md15030065md15030065The Influence of Spirulina platensis Filtrates on Caco-2 Proliferative Activity and Expression of Apoptosis-Related microRNAs and mRNAAgnieszka Śmieszek0Ewa Giezek1Martyna Chrapiec2Martyna Murat3Aleksandra Mucha4Izabela Michalak5Krzysztof Marycz6Department of Environment Hygiene and Animal Welfare, The Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, 38 C Chelmonskiego St., 50-630 Wroclaw, PolandDepartment of Environment Hygiene and Animal Welfare, The Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, 38 C Chelmonskiego St., 50-630 Wroclaw, PolandDepartment of Environment Hygiene and Animal Welfare, The Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, 38 C Chelmonskiego St., 50-630 Wroclaw, PolandDepartment of Environment Hygiene and Animal Welfare, The Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, 38 C Chelmonskiego St., 50-630 Wroclaw, PolandDepartment of Environment Hygiene and Animal Welfare, The Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, 38 C Chelmonskiego St., 50-630 Wroclaw, PolandDepartment of Advanced Material Technologies, Faculty of Chemistry, Wrocław University of Science and Technology, 54-066 Wrocław, PolandDepartment of Environment Hygiene and Animal Welfare, The Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, 38 C Chelmonskiego St., 50-630 Wroclaw, PolandSpirulina platensis (SP) is a blue-green microalga that has recently raised attention not only as a nutritional component, but also as a source of bioactivities that have therapeutic effects and may find application in medicine, including cancer treatment. In the present study we determined the cytotoxic effect of S. platensis filtrates (SPF) on human colon cancer cell line Caco-2. Three concentrations of SPF were tested—1.25%, 2.5%, and 5% (v/v). We have found that the highest concentration of SPF exerts the strongest anti-proliferative and pro-apoptotic effect on Caco-2 cultures. The SPF negatively affected the morphology of Caco-2 causing colony shrinking and significant inhibition of metabolic and proliferative activity of cells. The wound-healing assay showed that the SPF impaired migratory capabilities of Caco-2. This observation was consistent with lowered mRNA levels for metalloproteinases. Furthermore, SPF decreased the transcript level of pro-survival genes (cyclin D1, surviving, and c-Myc) and reduced the autocrine secretion of Wnt-10b. The cytotoxic effect of SPF involved the modulation of the Bax and Bcl-2 ratio and a decrease of mitochondrial activity, and was related with increased levels of intracellular reactive oxygen species (ROS) and nitric oxide (NO). Moreover, the SPF also caused an increased number of cells in the apoptotic sub-G0 phase and up-regulated expression of mir-145, simultaneously decreasing expression of mir-17 and 146. Obtained results indicate that SPF can be considered as an agent with anti-cancer properties that may be used for colon cancer prevention and treatment.http://www.mdpi.com/1660-3397/15/3/65Spirulinacolonhuman colon cancercytotoxic effectanti-cancer effectproliferationmorphologyapoptosis |
| spellingShingle | Agnieszka Śmieszek Ewa Giezek Martyna Chrapiec Martyna Murat Aleksandra Mucha Izabela Michalak Krzysztof Marycz The Influence of Spirulina platensis Filtrates on Caco-2 Proliferative Activity and Expression of Apoptosis-Related microRNAs and mRNA Marine Drugs Spirulina colon human colon cancer cytotoxic effect anti-cancer effect proliferation morphology apoptosis |
| title | The Influence of Spirulina platensis Filtrates on Caco-2 Proliferative Activity and Expression of Apoptosis-Related microRNAs and mRNA |
| title_full | The Influence of Spirulina platensis Filtrates on Caco-2 Proliferative Activity and Expression of Apoptosis-Related microRNAs and mRNA |
| title_fullStr | The Influence of Spirulina platensis Filtrates on Caco-2 Proliferative Activity and Expression of Apoptosis-Related microRNAs and mRNA |
| title_full_unstemmed | The Influence of Spirulina platensis Filtrates on Caco-2 Proliferative Activity and Expression of Apoptosis-Related microRNAs and mRNA |
| title_short | The Influence of Spirulina platensis Filtrates on Caco-2 Proliferative Activity and Expression of Apoptosis-Related microRNAs and mRNA |
| title_sort | influence of spirulina platensis filtrates on caco 2 proliferative activity and expression of apoptosis related micrornas and mrna |
| topic | Spirulina colon human colon cancer cytotoxic effect anti-cancer effect proliferation morphology apoptosis |
| url | http://www.mdpi.com/1660-3397/15/3/65 |
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