Molecular Characterization of Novel Mycoviruses in Seven <i>Umbelopsis</i> Strains
The presence of viruses is less explored in Mucoromycota as compared to other fungal groups such as Ascomycota and Basidiomycota. Recently, more and more mycoviruses are identified from the early-diverging lineages of fungi. We have determined the genome of 11 novel dsRNA viruses in seven different...
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MDPI AG
2022-10-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/11/2343 |
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| author | Tünde Kartali Nóra Zsindely Ildikó Nyilasi Orsolya Németh Gergő Norbert Sávai Sándor Kocsubé Zoltán Lipinszki Roland Patai Krisztina Spisák Gábor Nagy László Bodai Csaba Vágvölgyi Tamás Papp |
| author_facet | Tünde Kartali Nóra Zsindely Ildikó Nyilasi Orsolya Németh Gergő Norbert Sávai Sándor Kocsubé Zoltán Lipinszki Roland Patai Krisztina Spisák Gábor Nagy László Bodai Csaba Vágvölgyi Tamás Papp |
| author_sort | Tünde Kartali |
| collection | DOAJ |
| description | The presence of viruses is less explored in Mucoromycota as compared to other fungal groups such as Ascomycota and Basidiomycota. Recently, more and more mycoviruses are identified from the early-diverging lineages of fungi. We have determined the genome of 11 novel dsRNA viruses in seven different <i>Umbelopsis</i> strains with next-generation sequencing (NGS). The identified viruses were named Umbelopsis ramanniana virus 5 (UrV5), 6a (UrV6a); 6b (UrV6b); 7 (UrV7); 8a (UrV8a); 8b (UrV8b); Umbelopsis gibberispora virus 1 (UgV1); 2 (UgV2) and Umbelopsis dimorpha virus 1a (UdV1a), 1b (UdV1b) and 2 (UdV2). All the newly identified viruses contain two open reading frames (ORFs), which putatively encode the coat protein (CP) and the RNA-dependent RNA polymerase (RdRp), respectively. Based on the phylogeny inferred from the RdRp sequences, eight viruses (UrV7, UrV8a, UrV8b, UgV1, UgV2, UdV1a, UdV1b and UdV2) belong to the genus <i>Totivirus</i>, while UrV5, UrV6a and UrV6b are placed into a yet unclassified but well-defined <i>Totiviridae</i>-related group. In UrV5, UgV1, UgV2, UrV8b, UdV1a, UdV2 and UdV1b, ORF2 is predicted to be translated as a fusion protein via a rare +1 (or −2) ribosomal frameshift, which is not characteristic to most members of the <i>Totivirus</i> genus. Virus particles 31 to 32 nm in diameter could be detected in the examined fungal strains by transmission electron microscopy. Through the identification and characterization of new viruses of Mucoromycota fungi, we can gain insight into the diversity of mycoviruses, as well as into their phylogeny and genome organization. |
| first_indexed | 2024-03-09T18:34:46Z |
| format | Article |
| id | doaj.art-e73fbcc46e3a4f23a2909ca0509f09b1 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T18:34:46Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | Viruses |
| spelling | doaj.art-e73fbcc46e3a4f23a2909ca0509f09b12023-11-24T07:15:39ZengMDPI AGViruses1999-49152022-10-011411234310.3390/v14112343Molecular Characterization of Novel Mycoviruses in Seven <i>Umbelopsis</i> StrainsTünde Kartali0Nóra Zsindely1Ildikó Nyilasi2Orsolya Németh3Gergő Norbert Sávai4Sándor Kocsubé5Zoltán Lipinszki6Roland Patai7Krisztina Spisák8Gábor Nagy9László Bodai10Csaba Vágvölgyi11Tamás Papp12ELKH-SZTE Fungal Pathogenicity Mechanisms Research Group, University of Szeged, 6726 Szeged, HungaryDepartment of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, HungaryMTA SZBK Lendület Laboratory of Cell Cycle Regulation, Institute of Biochemistry, Biological Research Centre, Eötvös Loránd Research Network (ELKH), 6726 Szeged, HungaryNeuronal Plasticity Research Group, Institute of Biophysics, Biological Research Centre, 6726 Szeged, HungaryNeuronal Plasticity Research Group, Institute of Biophysics, Biological Research Centre, 6726 Szeged, HungaryDepartment of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, HungaryDepartment of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, HungaryELKH-SZTE Fungal Pathogenicity Mechanisms Research Group, University of Szeged, 6726 Szeged, HungaryThe presence of viruses is less explored in Mucoromycota as compared to other fungal groups such as Ascomycota and Basidiomycota. Recently, more and more mycoviruses are identified from the early-diverging lineages of fungi. We have determined the genome of 11 novel dsRNA viruses in seven different <i>Umbelopsis</i> strains with next-generation sequencing (NGS). The identified viruses were named Umbelopsis ramanniana virus 5 (UrV5), 6a (UrV6a); 6b (UrV6b); 7 (UrV7); 8a (UrV8a); 8b (UrV8b); Umbelopsis gibberispora virus 1 (UgV1); 2 (UgV2) and Umbelopsis dimorpha virus 1a (UdV1a), 1b (UdV1b) and 2 (UdV2). All the newly identified viruses contain two open reading frames (ORFs), which putatively encode the coat protein (CP) and the RNA-dependent RNA polymerase (RdRp), respectively. Based on the phylogeny inferred from the RdRp sequences, eight viruses (UrV7, UrV8a, UrV8b, UgV1, UgV2, UdV1a, UdV1b and UdV2) belong to the genus <i>Totivirus</i>, while UrV5, UrV6a and UrV6b are placed into a yet unclassified but well-defined <i>Totiviridae</i>-related group. In UrV5, UgV1, UgV2, UrV8b, UdV1a, UdV2 and UdV1b, ORF2 is predicted to be translated as a fusion protein via a rare +1 (or −2) ribosomal frameshift, which is not characteristic to most members of the <i>Totivirus</i> genus. Virus particles 31 to 32 nm in diameter could be detected in the examined fungal strains by transmission electron microscopy. Through the identification and characterization of new viruses of Mucoromycota fungi, we can gain insight into the diversity of mycoviruses, as well as into their phylogeny and genome organization.https://www.mdpi.com/1999-4915/14/11/2343dsRNAmycovirus<i>Totiviridae</i>virus particles<i>Umbelopsis</i>Mucoromycota |
| spellingShingle | Tünde Kartali Nóra Zsindely Ildikó Nyilasi Orsolya Németh Gergő Norbert Sávai Sándor Kocsubé Zoltán Lipinszki Roland Patai Krisztina Spisák Gábor Nagy László Bodai Csaba Vágvölgyi Tamás Papp Molecular Characterization of Novel Mycoviruses in Seven <i>Umbelopsis</i> Strains Viruses dsRNA mycovirus <i>Totiviridae</i> virus particles <i>Umbelopsis</i> Mucoromycota |
| title | Molecular Characterization of Novel Mycoviruses in Seven <i>Umbelopsis</i> Strains |
| title_full | Molecular Characterization of Novel Mycoviruses in Seven <i>Umbelopsis</i> Strains |
| title_fullStr | Molecular Characterization of Novel Mycoviruses in Seven <i>Umbelopsis</i> Strains |
| title_full_unstemmed | Molecular Characterization of Novel Mycoviruses in Seven <i>Umbelopsis</i> Strains |
| title_short | Molecular Characterization of Novel Mycoviruses in Seven <i>Umbelopsis</i> Strains |
| title_sort | molecular characterization of novel mycoviruses in seven i umbelopsis i strains |
| topic | dsRNA mycovirus <i>Totiviridae</i> virus particles <i>Umbelopsis</i> Mucoromycota |
| url | https://www.mdpi.com/1999-4915/14/11/2343 |
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