Changes in Dynamic Thiol/Disulfide Homeostasis, and Substance P, B-Endorphin and α-Tocopherol Concentrations in the Spinal Cord of Chronically Lame Dairy Cows
Initial lameness inflammation leads to chronic lameness and development of chronic pain due to the release of pro-inflammatory mediators such as reactive oxygen species (ROS), which are implicated in the transition from acute to chronic pain, and free radical scavengers countering thiol, substance P...
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MDPI AG
2023-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/10/1620 |
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| author | Heine Müller Daniel Herzberg Ricardo Chihuailaf Pablo Strobel Marianne Werner Hedie Bustamante |
| author_facet | Heine Müller Daniel Herzberg Ricardo Chihuailaf Pablo Strobel Marianne Werner Hedie Bustamante |
| author_sort | Heine Müller |
| collection | DOAJ |
| description | Initial lameness inflammation leads to chronic lameness and development of chronic pain due to the release of pro-inflammatory mediators such as reactive oxygen species (ROS), which are implicated in the transition from acute to chronic pain, and free radical scavengers countering thiol, substance P (SP), and β-endorphin (BE). The present study aimed to evaluate the dynamic thiol–disulfide homeostasis, α-tocopherol concentrations and SP and BE concentrations in the spinal cord of chronically lame dairy cows. Ten lame and 10 non-lame cows with a parity range of 2–6 were selected for the study. Lame cows had a history of up to 3 months of lameness. Spinal cord samples were obtained from the L2 to L4 lumbar vertebrae aspect of each animal. A thiol–disulfide homeostasis assay was performed using absorbance, and the α-tocopherol concentration was determined by HPLC. SP and BE concentrations were measured using ELISA kits. The results indicated that SP and BE were significantly higher in the spinal cord of lame cows. In contrast, disulfide levels and α-tocopherol concentrations were significantly lower in the spinal cord of lame cows. In conclusion, disulfide levels and α-tocopherol concentrations indicated a defective antioxidant response in cows with chronic lameness. The results of SP and BE concentrations suggested chronic pain and a defective endogenous analgesic response. |
| first_indexed | 2024-03-11T04:01:06Z |
| format | Article |
| id | doaj.art-e740aed4adfb4d899147227e2b812eff |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T04:01:06Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-e740aed4adfb4d899147227e2b812eff2023-11-18T00:09:45ZengMDPI AGAnimals2076-26152023-05-011310162010.3390/ani13101620Changes in Dynamic Thiol/Disulfide Homeostasis, and Substance P, B-Endorphin and α-Tocopherol Concentrations in the Spinal Cord of Chronically Lame Dairy CowsHeine Müller0Daniel Herzberg1Ricardo Chihuailaf2Pablo Strobel3Marianne Werner4Hedie Bustamante5Graduate School, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia 5110566, ChileGraduate School, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia 5110566, ChileVeterinary Clinical Sciences Institute, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia 5110566, ChileAnimal Science Institute, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia 5110566, ChileAnimal Science Institute, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia 5110566, ChileVeterinary Clinical Sciences Institute, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia 5110566, ChileInitial lameness inflammation leads to chronic lameness and development of chronic pain due to the release of pro-inflammatory mediators such as reactive oxygen species (ROS), which are implicated in the transition from acute to chronic pain, and free radical scavengers countering thiol, substance P (SP), and β-endorphin (BE). The present study aimed to evaluate the dynamic thiol–disulfide homeostasis, α-tocopherol concentrations and SP and BE concentrations in the spinal cord of chronically lame dairy cows. Ten lame and 10 non-lame cows with a parity range of 2–6 were selected for the study. Lame cows had a history of up to 3 months of lameness. Spinal cord samples were obtained from the L2 to L4 lumbar vertebrae aspect of each animal. A thiol–disulfide homeostasis assay was performed using absorbance, and the α-tocopherol concentration was determined by HPLC. SP and BE concentrations were measured using ELISA kits. The results indicated that SP and BE were significantly higher in the spinal cord of lame cows. In contrast, disulfide levels and α-tocopherol concentrations were significantly lower in the spinal cord of lame cows. In conclusion, disulfide levels and α-tocopherol concentrations indicated a defective antioxidant response in cows with chronic lameness. The results of SP and BE concentrations suggested chronic pain and a defective endogenous analgesic response.https://www.mdpi.com/2076-2615/13/10/1620dairy cowsthiolslamenesssubstance Pβ-endorphinα-tocopherol |
| spellingShingle | Heine Müller Daniel Herzberg Ricardo Chihuailaf Pablo Strobel Marianne Werner Hedie Bustamante Changes in Dynamic Thiol/Disulfide Homeostasis, and Substance P, B-Endorphin and α-Tocopherol Concentrations in the Spinal Cord of Chronically Lame Dairy Cows Animals dairy cows thiols lameness substance P β-endorphin α-tocopherol |
| title | Changes in Dynamic Thiol/Disulfide Homeostasis, and Substance P, B-Endorphin and α-Tocopherol Concentrations in the Spinal Cord of Chronically Lame Dairy Cows |
| title_full | Changes in Dynamic Thiol/Disulfide Homeostasis, and Substance P, B-Endorphin and α-Tocopherol Concentrations in the Spinal Cord of Chronically Lame Dairy Cows |
| title_fullStr | Changes in Dynamic Thiol/Disulfide Homeostasis, and Substance P, B-Endorphin and α-Tocopherol Concentrations in the Spinal Cord of Chronically Lame Dairy Cows |
| title_full_unstemmed | Changes in Dynamic Thiol/Disulfide Homeostasis, and Substance P, B-Endorphin and α-Tocopherol Concentrations in the Spinal Cord of Chronically Lame Dairy Cows |
| title_short | Changes in Dynamic Thiol/Disulfide Homeostasis, and Substance P, B-Endorphin and α-Tocopherol Concentrations in the Spinal Cord of Chronically Lame Dairy Cows |
| title_sort | changes in dynamic thiol disulfide homeostasis and substance p b endorphin and α tocopherol concentrations in the spinal cord of chronically lame dairy cows |
| topic | dairy cows thiols lameness substance P β-endorphin α-tocopherol |
| url | https://www.mdpi.com/2076-2615/13/10/1620 |
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