Oak (<i>Quercus robur</i>) Associated Endophytic <i>Paenibacillus</i> sp. Promotes Poplar (<i>Populus</i> spp.) Root Growth In Vitro
Soil fertilization is necessary for high-demand crop production in agriculture and forestry. Our current dependence on chemical fertilizers has significant harmful side effects. Biofertilization using microorganisms is a sustainable way to limit the need for chemical fertilizers in various enterpris...
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MDPI AG
2021-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/6/1151 |
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| author | Dorotėja Vaitiekūnaitė Sigutė Kuusienė Emilija Beniušytė |
| author_facet | Dorotėja Vaitiekūnaitė Sigutė Kuusienė Emilija Beniušytė |
| author_sort | Dorotėja Vaitiekūnaitė |
| collection | DOAJ |
| description | Soil fertilization is necessary for high-demand crop production in agriculture and forestry. Our current dependence on chemical fertilizers has significant harmful side effects. Biofertilization using microorganisms is a sustainable way to limit the need for chemical fertilizers in various enterprises. Most plant endophytic bacteria have thus far been unstudied for their plant growth promoting potential and hence present a novel niche for new biofertilizer strains. We isolated English oak (<i>Quercus robur</i>) endophytic bacteria and tested them for plant growth promoting traits (PGPTs) such as nitrogen fixation, phosphate mineralization/solubilization, siderophore and indole-3-acetic acid (IAA) production. We also investigated the effect the selected isolate had on poplar (<i>Populus</i> spp.) microshoot vegetative growth parameters in vitro. In total 48 bacterial strains were isolated, attributed to <i>Bacillus</i>, <i>Delftia</i>, <i>Paenibacillus</i>, <i>Pantoea</i> and <i>Pseudomonas</i> genera. All the isolates displayed at least three PGPTs, with 39.6% of the isolates displaying all five (all were <i>Pseudomonas</i> spp.) and 18.75% displaying four. Based on relative abundance, <i>Paenibacillus</i> sp. isolate was selected for the poplar microshoot inoculation study. The isolate had a significant positive effect on poplar microshoot root growth and development. Two tested poplar genotypes both had increased lateral root number and density, fresh and dry root biomass. Furthermore, one genotype had increased length and number of adventitious roots as well as a decrease in fresh aboveground biomass. The root enhancement was attributed to IAA production. We propose this isolate for further studies as a potential biofertilizer. |
| first_indexed | 2024-03-10T10:59:00Z |
| format | Article |
| id | doaj.art-e7433a25e2604681ba4a87d9581f5981 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T10:59:00Z |
| publishDate | 2021-05-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-e7433a25e2604681ba4a87d9581f59812023-11-21T21:40:50ZengMDPI AGMicroorganisms2076-26072021-05-0196115110.3390/microorganisms9061151Oak (<i>Quercus robur</i>) Associated Endophytic <i>Paenibacillus</i> sp. Promotes Poplar (<i>Populus</i> spp.) Root Growth In VitroDorotėja Vaitiekūnaitė0Sigutė Kuusienė1Emilija Beniušytė2Lithuanian Research Centre for Agriculture and Forestry, Laboratory of Forest Plant Biotechnology, Institute of Forestry, Liepu st. 1, Girionys, LT-53101 Šlienava, LithuaniaLithuanian Research Centre for Agriculture and Forestry, Laboratory of Forest Plant Biotechnology, Institute of Forestry, Liepu st. 1, Girionys, LT-53101 Šlienava, LithuaniaFaculty of Natural Sciences, Vytautas Magnus University, Universiteto st. 10, Akademija, LT-53361 Ringaudai, LithuaniaSoil fertilization is necessary for high-demand crop production in agriculture and forestry. Our current dependence on chemical fertilizers has significant harmful side effects. Biofertilization using microorganisms is a sustainable way to limit the need for chemical fertilizers in various enterprises. Most plant endophytic bacteria have thus far been unstudied for their plant growth promoting potential and hence present a novel niche for new biofertilizer strains. We isolated English oak (<i>Quercus robur</i>) endophytic bacteria and tested them for plant growth promoting traits (PGPTs) such as nitrogen fixation, phosphate mineralization/solubilization, siderophore and indole-3-acetic acid (IAA) production. We also investigated the effect the selected isolate had on poplar (<i>Populus</i> spp.) microshoot vegetative growth parameters in vitro. In total 48 bacterial strains were isolated, attributed to <i>Bacillus</i>, <i>Delftia</i>, <i>Paenibacillus</i>, <i>Pantoea</i> and <i>Pseudomonas</i> genera. All the isolates displayed at least three PGPTs, with 39.6% of the isolates displaying all five (all were <i>Pseudomonas</i> spp.) and 18.75% displaying four. Based on relative abundance, <i>Paenibacillus</i> sp. isolate was selected for the poplar microshoot inoculation study. The isolate had a significant positive effect on poplar microshoot root growth and development. Two tested poplar genotypes both had increased lateral root number and density, fresh and dry root biomass. Furthermore, one genotype had increased length and number of adventitious roots as well as a decrease in fresh aboveground biomass. The root enhancement was attributed to IAA production. We propose this isolate for further studies as a potential biofertilizer.https://www.mdpi.com/2076-2607/9/6/1151biofertilizer<i>Paenibacillus</i><i>Populus</i><i>Quercus robur</i>endophytebacteria |
| spellingShingle | Dorotėja Vaitiekūnaitė Sigutė Kuusienė Emilija Beniušytė Oak (<i>Quercus robur</i>) Associated Endophytic <i>Paenibacillus</i> sp. Promotes Poplar (<i>Populus</i> spp.) Root Growth In Vitro Microorganisms biofertilizer <i>Paenibacillus</i> <i>Populus</i> <i>Quercus robur</i> endophyte bacteria |
| title | Oak (<i>Quercus robur</i>) Associated Endophytic <i>Paenibacillus</i> sp. Promotes Poplar (<i>Populus</i> spp.) Root Growth In Vitro |
| title_full | Oak (<i>Quercus robur</i>) Associated Endophytic <i>Paenibacillus</i> sp. Promotes Poplar (<i>Populus</i> spp.) Root Growth In Vitro |
| title_fullStr | Oak (<i>Quercus robur</i>) Associated Endophytic <i>Paenibacillus</i> sp. Promotes Poplar (<i>Populus</i> spp.) Root Growth In Vitro |
| title_full_unstemmed | Oak (<i>Quercus robur</i>) Associated Endophytic <i>Paenibacillus</i> sp. Promotes Poplar (<i>Populus</i> spp.) Root Growth In Vitro |
| title_short | Oak (<i>Quercus robur</i>) Associated Endophytic <i>Paenibacillus</i> sp. Promotes Poplar (<i>Populus</i> spp.) Root Growth In Vitro |
| title_sort | oak i quercus robur i associated endophytic i paenibacillus i sp promotes poplar i populus i spp root growth in vitro |
| topic | biofertilizer <i>Paenibacillus</i> <i>Populus</i> <i>Quercus robur</i> endophyte bacteria |
| url | https://www.mdpi.com/2076-2607/9/6/1151 |
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