Fixed-Target Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II
Serial crystallography (SX) enables the determination of the structure of macromolecules or small molecules with minimal radiation damage. In particular, biomolecule structures determined using the SX technique have the advantage of providing room-temperature crystal structures with high biological...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Crystals |
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| Online Access: | https://www.mdpi.com/2073-4352/13/11/1544 |
| _version_ | 1797459709177888768 |
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| author | Yongsam Kim Ki Hyun Nam |
| author_facet | Yongsam Kim Ki Hyun Nam |
| author_sort | Yongsam Kim |
| collection | DOAJ |
| description | Serial crystallography (SX) enables the determination of the structure of macromolecules or small molecules with minimal radiation damage. In particular, biomolecule structures determined using the SX technique have the advantage of providing room-temperature crystal structures with high biological relevance. The SX technique requires numerous crystals to be collected to complete three-dimensional structural information. To minimize crystal sample consumption, we introduced SX data collection with fixed-target (FT) pink-beam serial synchrotron crystallography (SSX) at the 1C beamline of Pohang Light Source II. A new sample holder consisting of a magnetic frame with a nylon mesh was developed for easy sample handling. The FT-pink-SSX diffraction data were collected by continuously scanning X-rays using a stepping motor. The room-temperature structures of glucose isomerase and lysozyme were successfully determined at a resolution of 1.7 and 2.2 Å, respectively. The use of pink-beam FT-SSX in experimental applications and data acquisition for large beam sizes is discussed. Our results provide useful information for future pink-beam SSX and SX data collection using large X-ray beams. |
| first_indexed | 2024-03-09T16:55:13Z |
| format | Article |
| id | doaj.art-e74398f2016b4fa7be7f075773e5355e |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-03-09T16:55:13Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-e74398f2016b4fa7be7f075773e5355e2023-11-24T14:36:38ZengMDPI AGCrystals2073-43522023-10-011311154410.3390/cryst13111544Fixed-Target Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source IIYongsam Kim0Ki Hyun Nam1Pohang Accelerator Laboratory, Pohang University of Science and Technology, Pohang 37673, Republic of KoreaCollege of General Education, Kookmin University, Seoul 02707, Republic of KoreaSerial crystallography (SX) enables the determination of the structure of macromolecules or small molecules with minimal radiation damage. In particular, biomolecule structures determined using the SX technique have the advantage of providing room-temperature crystal structures with high biological relevance. The SX technique requires numerous crystals to be collected to complete three-dimensional structural information. To minimize crystal sample consumption, we introduced SX data collection with fixed-target (FT) pink-beam serial synchrotron crystallography (SSX) at the 1C beamline of Pohang Light Source II. A new sample holder consisting of a magnetic frame with a nylon mesh was developed for easy sample handling. The FT-pink-SSX diffraction data were collected by continuously scanning X-rays using a stepping motor. The room-temperature structures of glucose isomerase and lysozyme were successfully determined at a resolution of 1.7 and 2.2 Å, respectively. The use of pink-beam FT-SSX in experimental applications and data acquisition for large beam sizes is discussed. Our results provide useful information for future pink-beam SSX and SX data collection using large X-ray beams.https://www.mdpi.com/2073-4352/13/11/1544serial crystallographypink-beamfixed-target scanningroom-temperaturesynchrotron |
| spellingShingle | Yongsam Kim Ki Hyun Nam Fixed-Target Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II Crystals serial crystallography pink-beam fixed-target scanning room-temperature synchrotron |
| title | Fixed-Target Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II |
| title_full | Fixed-Target Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II |
| title_fullStr | Fixed-Target Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II |
| title_full_unstemmed | Fixed-Target Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II |
| title_short | Fixed-Target Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II |
| title_sort | fixed target pink beam serial synchrotron crystallography at pohang light source ii |
| topic | serial crystallography pink-beam fixed-target scanning room-temperature synchrotron |
| url | https://www.mdpi.com/2073-4352/13/11/1544 |
| work_keys_str_mv | AT yongsamkim fixedtargetpinkbeamserialsynchrotroncrystallographyatpohanglightsourceii AT kihyunnam fixedtargetpinkbeamserialsynchrotroncrystallographyatpohanglightsourceii |