Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making
This study aimed to compare the SARS-CoV-2 nucleic acid detection results of the BD MAX™ System and other platforms to formulate an optimized laboratory verification process. The re-examination of 400 samples determined as positive by BD MAX™ indicated that the inconsistency rate between BD MAX™ and...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/13/3/683 |
| _version_ | 1827749070367621120 |
|---|---|
| author | Wan-Wen Yang Chin-Wen Hsu Yu-Ju Chan Shih-Bin Su I-Jung Feng Chia-Yi Hou Chien-Yuan Huang |
| author_facet | Wan-Wen Yang Chin-Wen Hsu Yu-Ju Chan Shih-Bin Su I-Jung Feng Chia-Yi Hou Chien-Yuan Huang |
| author_sort | Wan-Wen Yang |
| collection | DOAJ |
| description | This study aimed to compare the SARS-CoV-2 nucleic acid detection results of the BD MAX™ System and other platforms to formulate an optimized laboratory verification process. The re-examination of 400 samples determined as positive by BD MAX™ indicated that the inconsistency rate between BD MAX™ and the other platforms was 65.8%; the inconsistency rate of single-gene-positive results was as high as 99.2%. A receiver operating characteristic curve was drawn for the relative light unit (RLU) values of samples positive for a single gene, and RLU 800 was used as the cutoff. After setting the retest standard as single-gene positive and RLU ≥ 800, the number of the 260 BD MAX™ single-gene positives that needed to be confirmed again was 36 (13.8%) and the number that could be directly reported as negative was 224 (86.2%). This verification process can shorten the reporting period and speed up the epidemic adjustment time and turnover rate of special wards, thereby improving SARS-CoV-2 detection efficiency and clinical decision-making. |
| first_indexed | 2024-03-11T06:17:44Z |
| format | Article |
| id | doaj.art-e7450fd05e8043cca59e7bd1509d2905 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T06:17:44Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-e7450fd05e8043cca59e7bd1509d29052023-11-17T12:10:55ZengMDPI AGLife2075-17292023-03-0113368310.3390/life13030683Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-MakingWan-Wen Yang0Chin-Wen Hsu1Yu-Ju Chan2Shih-Bin Su3I-Jung Feng4Chia-Yi Hou5Chien-Yuan Huang6Department of Clinical Pathology, Chi-Mei Medical Center, Liouying, Tainan 736402, TaiwanDepartment of Family Medicine, Chi-Mei Medical Center, Liouying, Tainan 736402, TaiwanDepartment of Family Medicine, Chi-Mei Medical Center, Liouying, Tainan 736402, TaiwanDivision of Occupational Medicine, Chi-Mei Medical Center, Liouying, Tainan 736402, TaiwanInstitute of Precision Medicine, National Sun Yat-sen University, Kaohsiung 804201, TaiwanDepartment of Clinical Pathology, Chi-Mei Medical Center, Liouying, Tainan 736402, TaiwanDivision of Occupational Medicine, Chi-Mei Medical Center, Liouying, Tainan 736402, TaiwanThis study aimed to compare the SARS-CoV-2 nucleic acid detection results of the BD MAX™ System and other platforms to formulate an optimized laboratory verification process. The re-examination of 400 samples determined as positive by BD MAX™ indicated that the inconsistency rate between BD MAX™ and the other platforms was 65.8%; the inconsistency rate of single-gene-positive results was as high as 99.2%. A receiver operating characteristic curve was drawn for the relative light unit (RLU) values of samples positive for a single gene, and RLU 800 was used as the cutoff. After setting the retest standard as single-gene positive and RLU ≥ 800, the number of the 260 BD MAX™ single-gene positives that needed to be confirmed again was 36 (13.8%) and the number that could be directly reported as negative was 224 (86.2%). This verification process can shorten the reporting period and speed up the epidemic adjustment time and turnover rate of special wards, thereby improving SARS-CoV-2 detection efficiency and clinical decision-making.https://www.mdpi.com/2075-1729/13/3/683SARS-CoV-2relative light unitclinical decision-making |
| spellingShingle | Wan-Wen Yang Chin-Wen Hsu Yu-Ju Chan Shih-Bin Su I-Jung Feng Chia-Yi Hou Chien-Yuan Huang Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making Life SARS-CoV-2 relative light unit clinical decision-making |
| title | Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making |
| title_full | Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making |
| title_fullStr | Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making |
| title_full_unstemmed | Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making |
| title_short | Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making |
| title_sort | using real time pcr fluorescence reaction values to improve sars cov 2 virus detection and benefit clinical decision making |
| topic | SARS-CoV-2 relative light unit clinical decision-making |
| url | https://www.mdpi.com/2075-1729/13/3/683 |
| work_keys_str_mv | AT wanwenyang usingrealtimepcrfluorescencereactionvaluestoimprovesarscov2virusdetectionandbenefitclinicaldecisionmaking AT chinwenhsu usingrealtimepcrfluorescencereactionvaluestoimprovesarscov2virusdetectionandbenefitclinicaldecisionmaking AT yujuchan usingrealtimepcrfluorescencereactionvaluestoimprovesarscov2virusdetectionandbenefitclinicaldecisionmaking AT shihbinsu usingrealtimepcrfluorescencereactionvaluestoimprovesarscov2virusdetectionandbenefitclinicaldecisionmaking AT ijungfeng usingrealtimepcrfluorescencereactionvaluestoimprovesarscov2virusdetectionandbenefitclinicaldecisionmaking AT chiayihou usingrealtimepcrfluorescencereactionvaluestoimprovesarscov2virusdetectionandbenefitclinicaldecisionmaking AT chienyuanhuang usingrealtimepcrfluorescencereactionvaluestoimprovesarscov2virusdetectionandbenefitclinicaldecisionmaking |