Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making

This study aimed to compare the SARS-CoV-2 nucleic acid detection results of the BD MAX™ System and other platforms to formulate an optimized laboratory verification process. The re-examination of 400 samples determined as positive by BD MAX™ indicated that the inconsistency rate between BD MAX™ and...

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Main Authors: Wan-Wen Yang, Chin-Wen Hsu, Yu-Ju Chan, Shih-Bin Su, I-Jung Feng, Chia-Yi Hou, Chien-Yuan Huang
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:Life
Subjects:
Online Access:https://www.mdpi.com/2075-1729/13/3/683
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author Wan-Wen Yang
Chin-Wen Hsu
Yu-Ju Chan
Shih-Bin Su
I-Jung Feng
Chia-Yi Hou
Chien-Yuan Huang
author_facet Wan-Wen Yang
Chin-Wen Hsu
Yu-Ju Chan
Shih-Bin Su
I-Jung Feng
Chia-Yi Hou
Chien-Yuan Huang
author_sort Wan-Wen Yang
collection DOAJ
description This study aimed to compare the SARS-CoV-2 nucleic acid detection results of the BD MAX™ System and other platforms to formulate an optimized laboratory verification process. The re-examination of 400 samples determined as positive by BD MAX™ indicated that the inconsistency rate between BD MAX™ and the other platforms was 65.8%; the inconsistency rate of single-gene-positive results was as high as 99.2%. A receiver operating characteristic curve was drawn for the relative light unit (RLU) values of samples positive for a single gene, and RLU 800 was used as the cutoff. After setting the retest standard as single-gene positive and RLU ≥ 800, the number of the 260 BD MAX™ single-gene positives that needed to be confirmed again was 36 (13.8%) and the number that could be directly reported as negative was 224 (86.2%). This verification process can shorten the reporting period and speed up the epidemic adjustment time and turnover rate of special wards, thereby improving SARS-CoV-2 detection efficiency and clinical decision-making.
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spelling doaj.art-e7450fd05e8043cca59e7bd1509d29052023-11-17T12:10:55ZengMDPI AGLife2075-17292023-03-0113368310.3390/life13030683Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-MakingWan-Wen Yang0Chin-Wen Hsu1Yu-Ju Chan2Shih-Bin Su3I-Jung Feng4Chia-Yi Hou5Chien-Yuan Huang6Department of Clinical Pathology, Chi-Mei Medical Center, Liouying, Tainan 736402, TaiwanDepartment of Family Medicine, Chi-Mei Medical Center, Liouying, Tainan 736402, TaiwanDepartment of Family Medicine, Chi-Mei Medical Center, Liouying, Tainan 736402, TaiwanDivision of Occupational Medicine, Chi-Mei Medical Center, Liouying, Tainan 736402, TaiwanInstitute of Precision Medicine, National Sun Yat-sen University, Kaohsiung 804201, TaiwanDepartment of Clinical Pathology, Chi-Mei Medical Center, Liouying, Tainan 736402, TaiwanDivision of Occupational Medicine, Chi-Mei Medical Center, Liouying, Tainan 736402, TaiwanThis study aimed to compare the SARS-CoV-2 nucleic acid detection results of the BD MAX™ System and other platforms to formulate an optimized laboratory verification process. The re-examination of 400 samples determined as positive by BD MAX™ indicated that the inconsistency rate between BD MAX™ and the other platforms was 65.8%; the inconsistency rate of single-gene-positive results was as high as 99.2%. A receiver operating characteristic curve was drawn for the relative light unit (RLU) values of samples positive for a single gene, and RLU 800 was used as the cutoff. After setting the retest standard as single-gene positive and RLU ≥ 800, the number of the 260 BD MAX™ single-gene positives that needed to be confirmed again was 36 (13.8%) and the number that could be directly reported as negative was 224 (86.2%). This verification process can shorten the reporting period and speed up the epidemic adjustment time and turnover rate of special wards, thereby improving SARS-CoV-2 detection efficiency and clinical decision-making.https://www.mdpi.com/2075-1729/13/3/683SARS-CoV-2relative light unitclinical decision-making
spellingShingle Wan-Wen Yang
Chin-Wen Hsu
Yu-Ju Chan
Shih-Bin Su
I-Jung Feng
Chia-Yi Hou
Chien-Yuan Huang
Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making
Life
SARS-CoV-2
relative light unit
clinical decision-making
title Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making
title_full Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making
title_fullStr Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making
title_full_unstemmed Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making
title_short Using Real-Time PCR Fluorescence Reaction Values to Improve SARS-CoV-2 Virus Detection and Benefit Clinical Decision-Making
title_sort using real time pcr fluorescence reaction values to improve sars cov 2 virus detection and benefit clinical decision making
topic SARS-CoV-2
relative light unit
clinical decision-making
url https://www.mdpi.com/2075-1729/13/3/683
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