Drought stress stimulates endocytosis and modifies membrane lipid order of rhizodermal cells of Medicago truncatula in a genotype-dependent manner
Abstract Background Drought stress negatively affects plant growth and productivity. Plants sense soil drought at the root level but the underlying mechanisms remain unclear. At the cell level, we aim to reveal the short-term root perception of drought stress through membrane dynamics. Results In ou...
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1814-y |
| _version_ | 1818534759517650944 |
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| author | Mégane Couchoud Christophe Der Sylvie Girodet Vanessa Vernoud Marion Prudent Nathalie Leborgne-Castel |
| author_facet | Mégane Couchoud Christophe Der Sylvie Girodet Vanessa Vernoud Marion Prudent Nathalie Leborgne-Castel |
| author_sort | Mégane Couchoud |
| collection | DOAJ |
| description | Abstract Background Drought stress negatively affects plant growth and productivity. Plants sense soil drought at the root level but the underlying mechanisms remain unclear. At the cell level, we aim to reveal the short-term root perception of drought stress through membrane dynamics. Results In our study, 15 Medicago truncatula accessions were exposed to a polyethylene glycol (PEG)-induced drought stress, leading to contrasted ecophysiological responses, in particular related to root architecture plasticity. In the reference accession Jemalong A17, identified as drought susceptible, we analyzed lateral roots by imaging of membrane-localized fluorescent probes using confocal microscopy. We found that PEG stimulated endocytosis especially in cells belonging to the growth differentiation zone (GDZ). The mapping of membrane lipid order in cells along the root apex showed that membranes of root cap cells were more ordered than those of more differentiated cells. Moreover, PEG triggered a significant increase in membrane lipid order of rhizodermal cells from the GDZ. We initiated the membrane analysis in the drought resistant accession HM298, which did not reveal such membrane modifications in response to PEG. Conclusions Our data demonstrated that the plasma membranes of root cells from a susceptible genotype perceived drought stress by modulating their physical state both via a stimulation of endocytosis and a modification of the degree of lipid order, which could be proposed as mechanisms required for signal transduction. |
| first_indexed | 2024-12-11T18:15:45Z |
| format | Article |
| id | doaj.art-e7454fe3ed4c4b18b5028d15a1f09780 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-11T18:15:45Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-e7454fe3ed4c4b18b5028d15a1f097802022-12-22T00:55:26ZengBMCBMC Plant Biology1471-22292019-05-0119111410.1186/s12870-019-1814-yDrought stress stimulates endocytosis and modifies membrane lipid order of rhizodermal cells of Medicago truncatula in a genotype-dependent mannerMégane Couchoud0Christophe Der1Sylvie Girodet2Vanessa Vernoud3Marion Prudent4Nathalie Leborgne-Castel5Agroécologie, AgroSup Dijon, INRA, University of Bourgogne, University of Bourgogne Franche-ComtéAgroécologie, AgroSup Dijon, CNRS, INRA, University of Bourgogne, University of Bourgogne Franche-ComtéAgroécologie, AgroSup Dijon, INRA, University of Bourgogne, University of Bourgogne Franche-ComtéAgroécologie, AgroSup Dijon, INRA, University of Bourgogne, University of Bourgogne Franche-ComtéAgroécologie, AgroSup Dijon, INRA, University of Bourgogne, University of Bourgogne Franche-ComtéAgroécologie, AgroSup Dijon, CNRS, INRA, University of Bourgogne, University of Bourgogne Franche-ComtéAbstract Background Drought stress negatively affects plant growth and productivity. Plants sense soil drought at the root level but the underlying mechanisms remain unclear. At the cell level, we aim to reveal the short-term root perception of drought stress through membrane dynamics. Results In our study, 15 Medicago truncatula accessions were exposed to a polyethylene glycol (PEG)-induced drought stress, leading to contrasted ecophysiological responses, in particular related to root architecture plasticity. In the reference accession Jemalong A17, identified as drought susceptible, we analyzed lateral roots by imaging of membrane-localized fluorescent probes using confocal microscopy. We found that PEG stimulated endocytosis especially in cells belonging to the growth differentiation zone (GDZ). The mapping of membrane lipid order in cells along the root apex showed that membranes of root cap cells were more ordered than those of more differentiated cells. Moreover, PEG triggered a significant increase in membrane lipid order of rhizodermal cells from the GDZ. We initiated the membrane analysis in the drought resistant accession HM298, which did not reveal such membrane modifications in response to PEG. Conclusions Our data demonstrated that the plasma membranes of root cells from a susceptible genotype perceived drought stress by modulating their physical state both via a stimulation of endocytosis and a modification of the degree of lipid order, which could be proposed as mechanisms required for signal transduction.http://link.springer.com/article/10.1186/s12870-019-1814-yMedicago truncatulaRoot architecturePEGEndocytosisOrder degree of the membraneFluorescent dyes |
| spellingShingle | Mégane Couchoud Christophe Der Sylvie Girodet Vanessa Vernoud Marion Prudent Nathalie Leborgne-Castel Drought stress stimulates endocytosis and modifies membrane lipid order of rhizodermal cells of Medicago truncatula in a genotype-dependent manner BMC Plant Biology Medicago truncatula Root architecture PEG Endocytosis Order degree of the membrane Fluorescent dyes |
| title | Drought stress stimulates endocytosis and modifies membrane lipid order of rhizodermal cells of Medicago truncatula in a genotype-dependent manner |
| title_full | Drought stress stimulates endocytosis and modifies membrane lipid order of rhizodermal cells of Medicago truncatula in a genotype-dependent manner |
| title_fullStr | Drought stress stimulates endocytosis and modifies membrane lipid order of rhizodermal cells of Medicago truncatula in a genotype-dependent manner |
| title_full_unstemmed | Drought stress stimulates endocytosis and modifies membrane lipid order of rhizodermal cells of Medicago truncatula in a genotype-dependent manner |
| title_short | Drought stress stimulates endocytosis and modifies membrane lipid order of rhizodermal cells of Medicago truncatula in a genotype-dependent manner |
| title_sort | drought stress stimulates endocytosis and modifies membrane lipid order of rhizodermal cells of medicago truncatula in a genotype dependent manner |
| topic | Medicago truncatula Root architecture PEG Endocytosis Order degree of the membrane Fluorescent dyes |
| url | http://link.springer.com/article/10.1186/s12870-019-1814-y |
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