Insecticidal activity of essential oils from American native plants against Aedes aegypti (Diptera: Culicidae): an introduction to their possible mechanism of action
Abstract Searching for new bioactive molecules to design insecticides is a complex process since pesticides should be highly selective, active against the vector, and bio-safe for humans. Aiming to find natural compounds for mosquito control, we evaluated the insecticidal activity of essential oils...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-30046-8 |
| _version_ | 1797864866896150528 |
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| author | Jonny E. Duque Diana L. Urbina Luis C. Vesga Luis A. Ortiz-Rodríguez Thomas S. Vanegas Elena E. Stashenko Stelia C. Mendez-Sanchez |
| author_facet | Jonny E. Duque Diana L. Urbina Luis C. Vesga Luis A. Ortiz-Rodríguez Thomas S. Vanegas Elena E. Stashenko Stelia C. Mendez-Sanchez |
| author_sort | Jonny E. Duque |
| collection | DOAJ |
| description | Abstract Searching for new bioactive molecules to design insecticides is a complex process since pesticides should be highly selective, active against the vector, and bio-safe for humans. Aiming to find natural compounds for mosquito control, we evaluated the insecticidal activity of essential oils (EOs) from 20 American native plants against Aedes aegypti larvae using bioassay, biochemical, and in silico analyses. The highest larvicide activity was exhibited by EOs from Steiractinia aspera (LC50 = 42.4 µg/mL), Turnera diffusa (LC50 = 70.9 µg/mL), Piper aduncum (LC50 = 55.8 µg/mL), Lippia origanoides (chemotype thymol/carvacrol) (LC50 = 61.9 µg/mL), L. origanoides (chemotype carvacrol/thymol) (LC50 = 59.8 µg/mL), Hyptis dilatata (LC50 = 61.1 µg/mL), Elaphandra quinquenervis (LC50 = 61.1 µg/mL), and Calycolpus moritzianus (LC50 = 73.29 µg/mL) after 24 h. This biological activity may be related to the disruption of the electron transport chain through the mitochondrial protein complexes. We hypothesized that the observed EOs' effect is due to their major components, where computational approaches such as homology modeling and molecular docking may suggest the possible binding pose of secondary metabolites that inhibit the mitochondrial enzymes and acetylcholinesterase activity (AChE). Our results provided insights into the possible mechanism of action of EOs and their major compounds for new insecticide designs targeting the mitochondria and AChE activity in A. aegypti for effective and safe insecticide. |
| first_indexed | 2024-04-09T22:58:53Z |
| format | Article |
| id | doaj.art-e7464f8db1cf4281acf45e328822f55a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:58:53Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e7464f8db1cf4281acf45e328822f55a2023-03-22T11:06:47ZengNature PortfolioScientific Reports2045-23222023-02-0113111510.1038/s41598-023-30046-8Insecticidal activity of essential oils from American native plants against Aedes aegypti (Diptera: Culicidae): an introduction to their possible mechanism of actionJonny E. Duque0Diana L. Urbina1Luis C. Vesga2Luis A. Ortiz-Rodríguez3Thomas S. Vanegas4Elena E. Stashenko5Stelia C. Mendez-Sanchez6Centro de Investigaciones en Enfermedades Tropicales – Cintrop, Departamento de Ciencias Básicas, Escuela de Medicina, Universidad Industrial de SantanderGrupo de Investigación en Bioquímica y Microbiología (GIBIM), Escuela de Química, Universidad Industrial de SantanderGrupo de Investigación en Bioquímica y Microbiología (GIBIM), Escuela de Química, Universidad Industrial de SantanderCentro de Investigaciones en Enfermedades Tropicales – Cintrop, Departamento de Ciencias Básicas, Escuela de Medicina, Universidad Industrial de SantanderCentro de Investigaciones en Enfermedades Tropicales – Cintrop, Departamento de Ciencias Básicas, Escuela de Medicina, Universidad Industrial de SantanderCentro de Investigación en Biomoléculas – CIBIMOL y Centro Nacional de Investigación para la Agroindustrialización de Plantas Aromáticas y Medicinales Tropicales – CENIVAM, Universidad Industrial de SantanderGrupo de Investigación en Bioquímica y Microbiología (GIBIM), Escuela de Química, Universidad Industrial de SantanderAbstract Searching for new bioactive molecules to design insecticides is a complex process since pesticides should be highly selective, active against the vector, and bio-safe for humans. Aiming to find natural compounds for mosquito control, we evaluated the insecticidal activity of essential oils (EOs) from 20 American native plants against Aedes aegypti larvae using bioassay, biochemical, and in silico analyses. The highest larvicide activity was exhibited by EOs from Steiractinia aspera (LC50 = 42.4 µg/mL), Turnera diffusa (LC50 = 70.9 µg/mL), Piper aduncum (LC50 = 55.8 µg/mL), Lippia origanoides (chemotype thymol/carvacrol) (LC50 = 61.9 µg/mL), L. origanoides (chemotype carvacrol/thymol) (LC50 = 59.8 µg/mL), Hyptis dilatata (LC50 = 61.1 µg/mL), Elaphandra quinquenervis (LC50 = 61.1 µg/mL), and Calycolpus moritzianus (LC50 = 73.29 µg/mL) after 24 h. This biological activity may be related to the disruption of the electron transport chain through the mitochondrial protein complexes. We hypothesized that the observed EOs' effect is due to their major components, where computational approaches such as homology modeling and molecular docking may suggest the possible binding pose of secondary metabolites that inhibit the mitochondrial enzymes and acetylcholinesterase activity (AChE). Our results provided insights into the possible mechanism of action of EOs and their major compounds for new insecticide designs targeting the mitochondria and AChE activity in A. aegypti for effective and safe insecticide.https://doi.org/10.1038/s41598-023-30046-8 |
| spellingShingle | Jonny E. Duque Diana L. Urbina Luis C. Vesga Luis A. Ortiz-Rodríguez Thomas S. Vanegas Elena E. Stashenko Stelia C. Mendez-Sanchez Insecticidal activity of essential oils from American native plants against Aedes aegypti (Diptera: Culicidae): an introduction to their possible mechanism of action Scientific Reports |
| title | Insecticidal activity of essential oils from American native plants against Aedes aegypti (Diptera: Culicidae): an introduction to their possible mechanism of action |
| title_full | Insecticidal activity of essential oils from American native plants against Aedes aegypti (Diptera: Culicidae): an introduction to their possible mechanism of action |
| title_fullStr | Insecticidal activity of essential oils from American native plants against Aedes aegypti (Diptera: Culicidae): an introduction to their possible mechanism of action |
| title_full_unstemmed | Insecticidal activity of essential oils from American native plants against Aedes aegypti (Diptera: Culicidae): an introduction to their possible mechanism of action |
| title_short | Insecticidal activity of essential oils from American native plants against Aedes aegypti (Diptera: Culicidae): an introduction to their possible mechanism of action |
| title_sort | insecticidal activity of essential oils from american native plants against aedes aegypti diptera culicidae an introduction to their possible mechanism of action |
| url | https://doi.org/10.1038/s41598-023-30046-8 |
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