CRYAB predicts clinical prognosis and is associated with immunocyte infiltration in colorectal cancer
Background αB-Crystallin (CRYAB) is differentially expressed in various tumors. However, the correlation between CRYAB and immune cell infiltration in colorectal cancer (CRC) remains unclear. Materials & Methods Kaplan–Meier survival curves in The Cancer Genome Atlas (TCGA) were used to evaluate...
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PeerJ Inc.
2021-12-01
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| Online Access: | https://peerj.com/articles/12578.pdf |
| _version_ | 1797410525931372544 |
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| author | Junsheng Deng Xiaoli Chen Ting Zhan Mengge Chen Xisheng Yan Xiaodong Huang |
| author_facet | Junsheng Deng Xiaoli Chen Ting Zhan Mengge Chen Xisheng Yan Xiaodong Huang |
| author_sort | Junsheng Deng |
| collection | DOAJ |
| description | Background αB-Crystallin (CRYAB) is differentially expressed in various tumors. However, the correlation between CRYAB and immune cell infiltration in colorectal cancer (CRC) remains unclear. Materials & Methods Kaplan–Meier survival curves in The Cancer Genome Atlas (TCGA) were used to evaluate the relationship between CRYAB expression and both overall survival and progression-free survival. The relationships between CRYAB expression and infiltrating immune cells and their corresponding gene marker sets were examined using the TIMER database. Results The expression of CRYAB was lower in CRC tumor tissues than in normal tissues (P < 0.05). High CRYAB gene expression and high levels of CRYAB gene methylation were correlated with high-grade malignant tumors and more advanced tumor, nodes and metastasis (TNM) cancer stages. In addition, in colorectal cancer, there was a positive correlation between CRYAB expression and immune infiltrating cells including neutrophils, macrophages, CD8 + T cells, and CD4 + T cells, as well as immune-related genes including CD2, CD3D, and CD3E. Methylation sites such as cg13084335, cg15545878, cg13210534, and cg15318568 were positively correlated with low expression of CRYAB. Conclusion Because CRYAB likely plays an important role in immune cell infiltration, it may be a potential tumor-suppressor gene in CRC and a potential novel therapeutic target and predictive biomarker for colorectal cancer (CRC). |
| first_indexed | 2024-03-09T04:31:19Z |
| format | Article |
| id | doaj.art-e747a1dc8951489281f7251433739d90 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T04:31:19Z |
| publishDate | 2021-12-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-e747a1dc8951489281f7251433739d902023-12-03T13:36:15ZengPeerJ Inc.PeerJ2167-83592021-12-019e1257810.7717/peerj.12578CRYAB predicts clinical prognosis and is associated with immunocyte infiltration in colorectal cancerJunsheng Deng0Xiaoli Chen1Ting Zhan2Mengge Chen3Xisheng Yan4Xiaodong Huang5Gastroenterology, Tongren Hospital of Wuhan University, Wuhan, Hubei, ChinaGastroenterology, Tongren Hospital of Wuhan University, Wuhan, Hubei, ChinaGastroenterology, Tongren Hospital of Wuhan University, Wuhan, Hubei, ChinaGastroenterology, Tongren Hospital of Wuhan University, Wuhan, Hubei, ChinaTongren Hospital of Wuhan Unversity, Wuhan, Hubei, ChinaGastroenterology, Tongren Hospital of Wuhan University, Wuhan, Hubei, ChinaBackground αB-Crystallin (CRYAB) is differentially expressed in various tumors. However, the correlation between CRYAB and immune cell infiltration in colorectal cancer (CRC) remains unclear. Materials & Methods Kaplan–Meier survival curves in The Cancer Genome Atlas (TCGA) were used to evaluate the relationship between CRYAB expression and both overall survival and progression-free survival. The relationships between CRYAB expression and infiltrating immune cells and their corresponding gene marker sets were examined using the TIMER database. Results The expression of CRYAB was lower in CRC tumor tissues than in normal tissues (P < 0.05). High CRYAB gene expression and high levels of CRYAB gene methylation were correlated with high-grade malignant tumors and more advanced tumor, nodes and metastasis (TNM) cancer stages. In addition, in colorectal cancer, there was a positive correlation between CRYAB expression and immune infiltrating cells including neutrophils, macrophages, CD8 + T cells, and CD4 + T cells, as well as immune-related genes including CD2, CD3D, and CD3E. Methylation sites such as cg13084335, cg15545878, cg13210534, and cg15318568 were positively correlated with low expression of CRYAB. Conclusion Because CRYAB likely plays an important role in immune cell infiltration, it may be a potential tumor-suppressor gene in CRC and a potential novel therapeutic target and predictive biomarker for colorectal cancer (CRC).https://peerj.com/articles/12578.pdfCRYABColorectal cancerImmune cellsMethylationImmune genes |
| spellingShingle | Junsheng Deng Xiaoli Chen Ting Zhan Mengge Chen Xisheng Yan Xiaodong Huang CRYAB predicts clinical prognosis and is associated with immunocyte infiltration in colorectal cancer PeerJ CRYAB Colorectal cancer Immune cells Methylation Immune genes |
| title | CRYAB predicts clinical prognosis and is associated with immunocyte infiltration in colorectal cancer |
| title_full | CRYAB predicts clinical prognosis and is associated with immunocyte infiltration in colorectal cancer |
| title_fullStr | CRYAB predicts clinical prognosis and is associated with immunocyte infiltration in colorectal cancer |
| title_full_unstemmed | CRYAB predicts clinical prognosis and is associated with immunocyte infiltration in colorectal cancer |
| title_short | CRYAB predicts clinical prognosis and is associated with immunocyte infiltration in colorectal cancer |
| title_sort | cryab predicts clinical prognosis and is associated with immunocyte infiltration in colorectal cancer |
| topic | CRYAB Colorectal cancer Immune cells Methylation Immune genes |
| url | https://peerj.com/articles/12578.pdf |
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