Quantitative Analysis of Micro-CT Imaging and Histopathological Signatures of Experimental Arthritis in Rats
Micro-computed tomographic (micro-CT) imaging provides a unique opportunity to capture 3-D architectural information in bone samples. In this study of pathological joint changes in a rat model of adjuvant-induced arthritis (AA), quantitative analysis of bone volume and roughness were performed by mi...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publications
2004-10-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200404136 |
| _version_ | 1797288066844459008 |
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| author | Matthew D. Silva Anneli Savinainen Rasesh Kapadia Jason Ruan Elizabeth Siebert Nicole Avitahl Rebecca Mosher Karen Anderson Bruce Jaffee Lisa Schopf Sudeep Chandra |
| author_facet | Matthew D. Silva Anneli Savinainen Rasesh Kapadia Jason Ruan Elizabeth Siebert Nicole Avitahl Rebecca Mosher Karen Anderson Bruce Jaffee Lisa Schopf Sudeep Chandra |
| author_sort | Matthew D. Silva |
| collection | DOAJ |
| description | Micro-computed tomographic (micro-CT) imaging provides a unique opportunity to capture 3-D architectural information in bone samples. In this study of pathological joint changes in a rat model of adjuvant-induced arthritis (AA), quantitative analysis of bone volume and roughness were performed by micro-CT imaging and compared with histopathology methods and paw swelling measurement. Micro-CT imaging of excised rat hind paws ( n = 10) stored in formalin consisted of approximately 600 30-μm slices acquired on a 512 × 512 image matrix with isotropic resolution. Following imaging, the joints were scored from H&E stained sections for cartilage/bone erosion, pannus development, inflammation, and synovial hyperplasia. From micro-CT images, quantitative analysis of absolute bone volumes and bone roughness was performed. Bone erosion in the rat AA model is substantial, leading to a significant decline in tarsal volume (27%). The result of the custom bone roughness measurement indicated a 55% increase in surface roughness. Histological and paw volume analyses also demonstrated severe arthritic disease as compared to controls. Statistical analyses indicate correlations among bone volume, roughness, histology, and paw volume. These data demonstrate that the destructive progression of disease in a rat AA model can be quantified using 3-D micro-CT image analysis, which allows assessment of arthritic disease status and efficacy of experimental therapeutic agents. |
| first_indexed | 2024-03-07T18:43:57Z |
| format | Article |
| id | doaj.art-e74852415f1541ed9754ffd3ac44b8c3 |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2024-03-07T18:43:57Z |
| publishDate | 2004-10-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-e74852415f1541ed9754ffd3ac44b8c32024-03-02T03:15:56ZengSAGE PublicationsMolecular Imaging1536-01212004-10-01310.1162/1535350020040413610.1162_15353500200404136Quantitative Analysis of Micro-CT Imaging and Histopathological Signatures of Experimental Arthritis in RatsMatthew D. Silva0Anneli Savinainen1Rasesh Kapadia2Jason Ruan3Elizabeth Siebert4Nicole Avitahl5Rebecca Mosher6Karen Anderson7Bruce Jaffee8Lisa Schopf9Sudeep Chandra10Millennium Pharmaceuticals, Inc., Cambridge, MAMillennium Pharmaceuticals, Inc., Cambridge, MAScanco USA, Inc., Wayne, PAMillennium Pharmaceuticals, Inc., Cambridge, MAMillennium Pharmaceuticals, Inc., Cambridge, MAMillennium Pharmaceuticals, Inc., Cambridge, MAMillennium Pharmaceuticals, Inc., Cambridge, MAMillennium Pharmaceuticals, Inc., Cambridge, MAMillennium Pharmaceuticals, Inc., Cambridge, MAMillennium Pharmaceuticals, Inc., Cambridge, MAMillennium Pharmaceuticals, Inc., Cambridge, MAMicro-computed tomographic (micro-CT) imaging provides a unique opportunity to capture 3-D architectural information in bone samples. In this study of pathological joint changes in a rat model of adjuvant-induced arthritis (AA), quantitative analysis of bone volume and roughness were performed by micro-CT imaging and compared with histopathology methods and paw swelling measurement. Micro-CT imaging of excised rat hind paws ( n = 10) stored in formalin consisted of approximately 600 30-μm slices acquired on a 512 × 512 image matrix with isotropic resolution. Following imaging, the joints were scored from H&E stained sections for cartilage/bone erosion, pannus development, inflammation, and synovial hyperplasia. From micro-CT images, quantitative analysis of absolute bone volumes and bone roughness was performed. Bone erosion in the rat AA model is substantial, leading to a significant decline in tarsal volume (27%). The result of the custom bone roughness measurement indicated a 55% increase in surface roughness. Histological and paw volume analyses also demonstrated severe arthritic disease as compared to controls. Statistical analyses indicate correlations among bone volume, roughness, histology, and paw volume. These data demonstrate that the destructive progression of disease in a rat AA model can be quantified using 3-D micro-CT image analysis, which allows assessment of arthritic disease status and efficacy of experimental therapeutic agents.https://doi.org/10.1162/15353500200404136 |
| spellingShingle | Matthew D. Silva Anneli Savinainen Rasesh Kapadia Jason Ruan Elizabeth Siebert Nicole Avitahl Rebecca Mosher Karen Anderson Bruce Jaffee Lisa Schopf Sudeep Chandra Quantitative Analysis of Micro-CT Imaging and Histopathological Signatures of Experimental Arthritis in Rats Molecular Imaging |
| title | Quantitative Analysis of Micro-CT Imaging and Histopathological Signatures of Experimental Arthritis in Rats |
| title_full | Quantitative Analysis of Micro-CT Imaging and Histopathological Signatures of Experimental Arthritis in Rats |
| title_fullStr | Quantitative Analysis of Micro-CT Imaging and Histopathological Signatures of Experimental Arthritis in Rats |
| title_full_unstemmed | Quantitative Analysis of Micro-CT Imaging and Histopathological Signatures of Experimental Arthritis in Rats |
| title_short | Quantitative Analysis of Micro-CT Imaging and Histopathological Signatures of Experimental Arthritis in Rats |
| title_sort | quantitative analysis of micro ct imaging and histopathological signatures of experimental arthritis in rats |
| url | https://doi.org/10.1162/15353500200404136 |
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