The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization
Abstract Oocyte maturity is critical to the development potential of the embryo and pregnancy outcomes in natural and in-vitro fertilization (IVF). In IVF, oocyte maturity is typically evaluated using morphological criteria, although such assessment remains highly subjective. To identify reliable bi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-23737-1 |
| _version_ | 1811217182276714496 |
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| author | Kun-Jing Hong Jun-Jie Lin Liang-Hua Lin Tsung-Hsuan Lai |
| author_facet | Kun-Jing Hong Jun-Jie Lin Liang-Hua Lin Tsung-Hsuan Lai |
| author_sort | Kun-Jing Hong |
| collection | DOAJ |
| description | Abstract Oocyte maturity is critical to the development potential of the embryo and pregnancy outcomes in natural and in-vitro fertilization (IVF). In IVF, oocyte maturity is typically evaluated using morphological criteria, although such assessment remains highly subjective. To identify reliable biomarkers of oocyte maturity, this study investigates the relationship between follicular cytokine concentrations and oocyte maturity in IVF patients with different ovarian reserves. In this prospective study, follicular fluid was collected during oocyte retrieval and the concentrations of cytokines involved in ovarian folliculogenesis were determined. Follicular fluid cytokine concentrations were compared between participants in three groups according to serum anti-Mullerian hormone (AMH) concentration, as follows: low AMH, < 2 ng/mL; normal AMH, 2–5 ng/mL; and high AMH, > 5 ng/mL. Pearson's correlation coefficient analysis showed that the number of mature oocytes correlated positively and strongly with serum AMH level (r = 0.719; p < 0.01). The leptin concentration in follicular fluid was significantly higher in women with normal AMH level than in those with low or high levels. ROC curve analysis showed that the follicular fluid levels of leptin (area under ROC curve, 0.829; 95% confidence interval, 0.659–0.998; p < 0.01) and SCF (area under ROC curve, 0.706; 95% confidence interval, 0.491–0.921; p = 0.087) were the best predictors of oocyte maturity. At an optimal cut-off value of 16 ng/mL, leptin had positive predictive value (sensitivity) up to 70% and negative predictive value (specificity) of 91% for indicating oocyte maturity. The concentration of leptin in follicular fluid is closely related to ovarian reserve and may serve as a biomarker to predict oocyte maturity. |
| first_indexed | 2024-04-12T06:49:43Z |
| format | Article |
| id | doaj.art-e74ae17339b046a49ee0985ce2e76e69 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T06:49:43Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e74ae17339b046a49ee0985ce2e76e692022-12-22T03:43:25ZengNature PortfolioScientific Reports2045-23222022-11-0112111010.1038/s41598-022-23737-1The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilizationKun-Jing Hong0Jun-Jie Lin1Liang-Hua Lin2Tsung-Hsuan Lai3Cathay Medical Research Institute, Cathay General HospitalDepartment of Obstetrics and Gynecology, Assisted Reproductive Center, Cathay General HospitalDepartment of Obstetrics and Gynecology, Assisted Reproductive Center, Cathay General HospitalDepartment of Obstetrics and Gynecology, Assisted Reproductive Center, Cathay General HospitalAbstract Oocyte maturity is critical to the development potential of the embryo and pregnancy outcomes in natural and in-vitro fertilization (IVF). In IVF, oocyte maturity is typically evaluated using morphological criteria, although such assessment remains highly subjective. To identify reliable biomarkers of oocyte maturity, this study investigates the relationship between follicular cytokine concentrations and oocyte maturity in IVF patients with different ovarian reserves. In this prospective study, follicular fluid was collected during oocyte retrieval and the concentrations of cytokines involved in ovarian folliculogenesis were determined. Follicular fluid cytokine concentrations were compared between participants in three groups according to serum anti-Mullerian hormone (AMH) concentration, as follows: low AMH, < 2 ng/mL; normal AMH, 2–5 ng/mL; and high AMH, > 5 ng/mL. Pearson's correlation coefficient analysis showed that the number of mature oocytes correlated positively and strongly with serum AMH level (r = 0.719; p < 0.01). The leptin concentration in follicular fluid was significantly higher in women with normal AMH level than in those with low or high levels. ROC curve analysis showed that the follicular fluid levels of leptin (area under ROC curve, 0.829; 95% confidence interval, 0.659–0.998; p < 0.01) and SCF (area under ROC curve, 0.706; 95% confidence interval, 0.491–0.921; p = 0.087) were the best predictors of oocyte maturity. At an optimal cut-off value of 16 ng/mL, leptin had positive predictive value (sensitivity) up to 70% and negative predictive value (specificity) of 91% for indicating oocyte maturity. The concentration of leptin in follicular fluid is closely related to ovarian reserve and may serve as a biomarker to predict oocyte maturity.https://doi.org/10.1038/s41598-022-23737-1 |
| spellingShingle | Kun-Jing Hong Jun-Jie Lin Liang-Hua Lin Tsung-Hsuan Lai The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization Scientific Reports |
| title | The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization |
| title_full | The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization |
| title_fullStr | The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization |
| title_full_unstemmed | The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization |
| title_short | The intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in-vitro fertilization |
| title_sort | intrafollicular concentration of leptin as a potential biomarker to predict oocyte maturity in in vitro fertilization |
| url | https://doi.org/10.1038/s41598-022-23737-1 |
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