Allelic Variation for Prolamins in Spanish Durum Wheat Landraces and Its Relationship with Quality Traits
Wheat landraces are currently being explored mainly as a source of allelic variation related to crop resilience and low-input adaptation. Characterization of their high- and low-molecular weight glutenin subunits can aid breeders to select as donor local materials those ensuring good end-use propert...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-01-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/10/1/136 |
| _version_ | 1818459295494176768 |
|---|---|
| author | Efraín A. Chacón Francisco J. Vázquez Patricia Giraldo José M. Carrillo Elena Benavente Marta Rodríguez-Quijano |
| author_facet | Efraín A. Chacón Francisco J. Vázquez Patricia Giraldo José M. Carrillo Elena Benavente Marta Rodríguez-Quijano |
| author_sort | Efraín A. Chacón |
| collection | DOAJ |
| description | Wheat landraces are currently being explored mainly as a source of allelic variation related to crop resilience and low-input adaptation. Characterization of their high- and low-molecular weight glutenin subunits can aid breeders to select as donor local materials those ensuring good end-use properties in the derived elite cultivars. By using protein electrophoretic methods, we have determined the prolamin allelic profile of 116 Spanish durum wheat landraces. Their quality properties (as defined by grain protein content, sodium dodecyl sulfate (SDS) sedimentation volume and mixograph behavior) have also been assessed. The study has identified six novel glutenin alleles plus some other rare alleles some of which have been associated with improved durum wheat quality. Most of the novel variation detected needs to be characterized in a wider sample of varieties to establish any eventual beneficial effect on functional quality. Further analysis of the quality properties associated to specific allele combinations of <i>Glu-A3</i>, <i>Glu-B3</i>, and <i>Glu-A1</i> has disclosed some clues on the influence on quality of certain non-allelic interactions between these main prolamin-encoding loci. Some of the landraces, showing outstanding values for the gluten quality parameters analyzed, might be directly used by farmers interested in the cultivation of traditional varieties for specialized food markets. |
| first_indexed | 2024-12-14T23:12:05Z |
| format | Article |
| id | doaj.art-e74fa45fc4404520b247af7a14ed2e83 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-12-14T23:12:05Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-e74fa45fc4404520b247af7a14ed2e832022-12-21T22:44:11ZengMDPI AGAgronomy2073-43952020-01-0110113610.3390/agronomy10010136agronomy10010136Allelic Variation for Prolamins in Spanish Durum Wheat Landraces and Its Relationship with Quality TraitsEfraín A. Chacón0Francisco J. Vázquez1Patricia Giraldo2José M. Carrillo3Elena Benavente4Marta Rodríguez-Quijano5Department of Biotechnology-Plant Biology, School of Agricultural, Food and Biosystems Engineering, Universidad Politécnica de Madrid, 28040 Madrid, SpainDepartment of Biotechnology-Plant Biology, School of Agricultural, Food and Biosystems Engineering, Universidad Politécnica de Madrid, 28040 Madrid, SpainDepartment of Biotechnology-Plant Biology, School of Agricultural, Food and Biosystems Engineering, Universidad Politécnica de Madrid, 28040 Madrid, SpainDepartment of Biotechnology-Plant Biology, School of Agricultural, Food and Biosystems Engineering, Universidad Politécnica de Madrid, 28040 Madrid, SpainDepartment of Biotechnology-Plant Biology, School of Agricultural, Food and Biosystems Engineering, Universidad Politécnica de Madrid, 28040 Madrid, SpainDepartment of Biotechnology-Plant Biology, School of Agricultural, Food and Biosystems Engineering, Universidad Politécnica de Madrid, 28040 Madrid, SpainWheat landraces are currently being explored mainly as a source of allelic variation related to crop resilience and low-input adaptation. Characterization of their high- and low-molecular weight glutenin subunits can aid breeders to select as donor local materials those ensuring good end-use properties in the derived elite cultivars. By using protein electrophoretic methods, we have determined the prolamin allelic profile of 116 Spanish durum wheat landraces. Their quality properties (as defined by grain protein content, sodium dodecyl sulfate (SDS) sedimentation volume and mixograph behavior) have also been assessed. The study has identified six novel glutenin alleles plus some other rare alleles some of which have been associated with improved durum wheat quality. Most of the novel variation detected needs to be characterized in a wider sample of varieties to establish any eventual beneficial effect on functional quality. Further analysis of the quality properties associated to specific allele combinations of <i>Glu-A3</i>, <i>Glu-B3</i>, and <i>Glu-A1</i> has disclosed some clues on the influence on quality of certain non-allelic interactions between these main prolamin-encoding loci. Some of the landraces, showing outstanding values for the gluten quality parameters analyzed, might be directly used by farmers interested in the cultivation of traditional varieties for specialized food markets.https://www.mdpi.com/2073-4395/10/1/136<i>triticum turgidum</i>hmw gluteninslmw gluteninsgluten qualitynon-allelic interactions |
| spellingShingle | Efraín A. Chacón Francisco J. Vázquez Patricia Giraldo José M. Carrillo Elena Benavente Marta Rodríguez-Quijano Allelic Variation for Prolamins in Spanish Durum Wheat Landraces and Its Relationship with Quality Traits Agronomy <i>triticum turgidum</i> hmw glutenins lmw glutenins gluten quality non-allelic interactions |
| title | Allelic Variation for Prolamins in Spanish Durum Wheat Landraces and Its Relationship with Quality Traits |
| title_full | Allelic Variation for Prolamins in Spanish Durum Wheat Landraces and Its Relationship with Quality Traits |
| title_fullStr | Allelic Variation for Prolamins in Spanish Durum Wheat Landraces and Its Relationship with Quality Traits |
| title_full_unstemmed | Allelic Variation for Prolamins in Spanish Durum Wheat Landraces and Its Relationship with Quality Traits |
| title_short | Allelic Variation for Prolamins in Spanish Durum Wheat Landraces and Its Relationship with Quality Traits |
| title_sort | allelic variation for prolamins in spanish durum wheat landraces and its relationship with quality traits |
| topic | <i>triticum turgidum</i> hmw glutenins lmw glutenins gluten quality non-allelic interactions |
| url | https://www.mdpi.com/2073-4395/10/1/136 |
| work_keys_str_mv | AT efrainachacon allelicvariationforprolaminsinspanishdurumwheatlandracesanditsrelationshipwithqualitytraits AT franciscojvazquez allelicvariationforprolaminsinspanishdurumwheatlandracesanditsrelationshipwithqualitytraits AT patriciagiraldo allelicvariationforprolaminsinspanishdurumwheatlandracesanditsrelationshipwithqualitytraits AT josemcarrillo allelicvariationforprolaminsinspanishdurumwheatlandracesanditsrelationshipwithqualitytraits AT elenabenavente allelicvariationforprolaminsinspanishdurumwheatlandracesanditsrelationshipwithqualitytraits AT martarodriguezquijano allelicvariationforprolaminsinspanishdurumwheatlandracesanditsrelationshipwithqualitytraits |