Evaluation of Plant Growth-Promoting and Salinity Ameliorating Potential of Halophilic Bacteria Isolated From Saline Soil
Among the biotic and abiotic stress affecting the physical, chemical, and biological properties of soil, salinity is a major threat that leads to the desertification of cultivable land throughout the world. The existence of diverse and versatile microbial populations inhabiting the nutrient-rich soi...
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| Format: | Article |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.946217/full |
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| author | Chintan Kapadia Nafisa Patel Ankita Rana Harihar Vaidya Saleh Alfarraj Mohammad Javed Ansari Abdul Gafur Peter Poczai R. Z. Sayyed R. Z. Sayyed |
| author_facet | Chintan Kapadia Nafisa Patel Ankita Rana Harihar Vaidya Saleh Alfarraj Mohammad Javed Ansari Abdul Gafur Peter Poczai R. Z. Sayyed R. Z. Sayyed |
| author_sort | Chintan Kapadia |
| collection | DOAJ |
| description | Among the biotic and abiotic stress affecting the physical, chemical, and biological properties of soil, salinity is a major threat that leads to the desertification of cultivable land throughout the world. The existence of diverse and versatile microbial populations inhabiting the nutrient-rich soil and varied soil conditions affects the soil dynamism. A normal soil constitutes 600 million bacteria belonging to about 20,000 species, which is reduced to 1 million with 5,000–8,000 species in stress conditions. Plant growth-promoting rhizobacteria (PGPR) are in symbiotic association with the plant system, which helps in combating the abiotic stress and increases the overall productivity and yield. These microorganisms are actively associated with varied cellular communication processes through quorum sensing and secondary metabolites such as the production of Indole-3-acetic acid (IAA), exopolysaccharide (EPS) siderophore, ammonia, ACC deaminase, and solubilization of phosphate. The present study focused on the isolation, identification, and characterization of the microorganisms isolated from the seacoast of Dandi, Navsari. Twelve isolates exhibited PGP traits at a high salt concentration of 15–20%. AD9 isolate identified as Bacillus halotolerans showed a higher ammonia production (88 ± 1.73 μg/mL) and phosphate solubilization (86 ± 3.06 μg/mL) at 15% salt concentration, while AD32* (Bacillus sp. clone ADCNO) gave 42.67 ±1.20 μg/mL IAA production at 20% salt concentration. AD2 (Streptomyces sp. clone ADCNB) and AD26 (Achromobacter sp. clone ADCNI) showed ACC deaminase activity of 0.61 ± 0.12 and 0.60 ± 0.04 nM α-ketobutyrate/mg protein/h, respectively. AD32 (Bacillus sp. clone ADCNL) gave a high siderophore activity of 65.40 ± 1.65%. These isolates produced salinity ameliorating traits, total antioxidant activities, and antioxidant enzymes viz. superoxide dismutase (SOD), Glutathione oxidase (GSH), and catalase (CAT). Inoculation of the multipotent isolate that produced PGP traits and salinity ameliorating metabolites promoted the plant growth and development in rice under salinity stress conditions. These results in 50% more root length, 25.00% more plant dry weight, and 41% more tillers compared to its control. |
| first_indexed | 2024-12-11T17:08:36Z |
| format | Article |
| id | doaj.art-e752ac605dec4dcf9dbf9081fc5d92f7 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T17:08:36Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-e752ac605dec4dcf9dbf9081fc5d92f72022-12-22T00:57:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-07-011310.3389/fpls.2022.946217946217Evaluation of Plant Growth-Promoting and Salinity Ameliorating Potential of Halophilic Bacteria Isolated From Saline SoilChintan Kapadia0Nafisa Patel1Ankita Rana2Harihar Vaidya3Saleh Alfarraj4Mohammad Javed Ansari5Abdul Gafur6Peter Poczai7R. Z. Sayyed8R. Z. Sayyed9Department of Plant Molecular Biology and Biotechnology, ASPEE College of Horticulture and Forestry, Navsari Agricultural University, Navsari, IndiaNaran Lala College of Professional and Applied Sciences, Navsari, IndiaNaran Lala College of Professional and Applied Sciences, Navsari, IndiaDepartment of Plant Molecular Biology and Biotechnology, ASPEE College of Horticulture and Forestry, Navsari Agricultural University, Navsari, IndiaZoology Department, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Botany, Hindu College (Mahatma Jyotiba Phule Rohilkhand University Bareilly), Moradabad, IndiaSinarmas Forestry Corporate Research and Development, Perawang, IndonesiaFinnish Museum of Natural History, University of Helsinki, Helsinki, FinlandDepartment of Entomology, Asian PGPR Society for Sustainable Agriculture, Auburn University, Auburn, AL, United StatesDepartment of Microbiology, PSGVP Mandal's‘S I Patil Arts, G B Patel Science, and STKV Sangh Commerce College, Shahada, IndiaAmong the biotic and abiotic stress affecting the physical, chemical, and biological properties of soil, salinity is a major threat that leads to the desertification of cultivable land throughout the world. The existence of diverse and versatile microbial populations inhabiting the nutrient-rich soil and varied soil conditions affects the soil dynamism. A normal soil constitutes 600 million bacteria belonging to about 20,000 species, which is reduced to 1 million with 5,000–8,000 species in stress conditions. Plant growth-promoting rhizobacteria (PGPR) are in symbiotic association with the plant system, which helps in combating the abiotic stress and increases the overall productivity and yield. These microorganisms are actively associated with varied cellular communication processes through quorum sensing and secondary metabolites such as the production of Indole-3-acetic acid (IAA), exopolysaccharide (EPS) siderophore, ammonia, ACC deaminase, and solubilization of phosphate. The present study focused on the isolation, identification, and characterization of the microorganisms isolated from the seacoast of Dandi, Navsari. Twelve isolates exhibited PGP traits at a high salt concentration of 15–20%. AD9 isolate identified as Bacillus halotolerans showed a higher ammonia production (88 ± 1.73 μg/mL) and phosphate solubilization (86 ± 3.06 μg/mL) at 15% salt concentration, while AD32* (Bacillus sp. clone ADCNO) gave 42.67 ±1.20 μg/mL IAA production at 20% salt concentration. AD2 (Streptomyces sp. clone ADCNB) and AD26 (Achromobacter sp. clone ADCNI) showed ACC deaminase activity of 0.61 ± 0.12 and 0.60 ± 0.04 nM α-ketobutyrate/mg protein/h, respectively. AD32 (Bacillus sp. clone ADCNL) gave a high siderophore activity of 65.40 ± 1.65%. These isolates produced salinity ameliorating traits, total antioxidant activities, and antioxidant enzymes viz. superoxide dismutase (SOD), Glutathione oxidase (GSH), and catalase (CAT). Inoculation of the multipotent isolate that produced PGP traits and salinity ameliorating metabolites promoted the plant growth and development in rice under salinity stress conditions. These results in 50% more root length, 25.00% more plant dry weight, and 41% more tillers compared to its control.https://www.frontiersin.org/articles/10.3389/fpls.2022.946217/fullabiotic stressACC deaminaseantioxidant enzymesbiofilmIAAmicrobial diversity |
| spellingShingle | Chintan Kapadia Nafisa Patel Ankita Rana Harihar Vaidya Saleh Alfarraj Mohammad Javed Ansari Abdul Gafur Peter Poczai R. Z. Sayyed R. Z. Sayyed Evaluation of Plant Growth-Promoting and Salinity Ameliorating Potential of Halophilic Bacteria Isolated From Saline Soil Frontiers in Plant Science abiotic stress ACC deaminase antioxidant enzymes biofilm IAA microbial diversity |
| title | Evaluation of Plant Growth-Promoting and Salinity Ameliorating Potential of Halophilic Bacteria Isolated From Saline Soil |
| title_full | Evaluation of Plant Growth-Promoting and Salinity Ameliorating Potential of Halophilic Bacteria Isolated From Saline Soil |
| title_fullStr | Evaluation of Plant Growth-Promoting and Salinity Ameliorating Potential of Halophilic Bacteria Isolated From Saline Soil |
| title_full_unstemmed | Evaluation of Plant Growth-Promoting and Salinity Ameliorating Potential of Halophilic Bacteria Isolated From Saline Soil |
| title_short | Evaluation of Plant Growth-Promoting and Salinity Ameliorating Potential of Halophilic Bacteria Isolated From Saline Soil |
| title_sort | evaluation of plant growth promoting and salinity ameliorating potential of halophilic bacteria isolated from saline soil |
| topic | abiotic stress ACC deaminase antioxidant enzymes biofilm IAA microbial diversity |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.946217/full |
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