Simple, time-saving dye staining of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue.
A fixation-free and fast protein-staining method for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining and quick washing steps, which can be completed in 0.5 h. It has a sensitivity of 10 ng, comparable with that of conventio...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3151240?pdf=render |
| _version_ | 1818555712250314752 |
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| author | Wei-Hua Dong Tian-Yun Wang Fang Wang Jun-He Zhang |
| author_facet | Wei-Hua Dong Tian-Yun Wang Fang Wang Jun-He Zhang |
| author_sort | Wei-Hua Dong |
| collection | DOAJ |
| description | A fixation-free and fast protein-staining method for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining and quick washing steps, which can be completed in 0.5 h. It has a sensitivity of 10 ng, comparable with that of conventional Coomassie Brilliant Blue G staining with phosphoric acid in the staining solution. In addition, the dye stain does not contain any amount of acid and methanol, such as phosphoric acid. Considering the speed, simplicity, and low cost, the dye stain may be of more practical value than other dye-based protein stains in routine proteomic research. |
| first_indexed | 2024-12-12T09:57:11Z |
| format | Article |
| id | doaj.art-e7560fc711814b81921fcb9a8ebe1837 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T09:57:11Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e7560fc711814b81921fcb9a8ebe18372022-12-22T00:28:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2239410.1371/journal.pone.0022394Simple, time-saving dye staining of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue.Wei-Hua DongTian-Yun WangFang WangJun-He ZhangA fixation-free and fast protein-staining method for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining and quick washing steps, which can be completed in 0.5 h. It has a sensitivity of 10 ng, comparable with that of conventional Coomassie Brilliant Blue G staining with phosphoric acid in the staining solution. In addition, the dye stain does not contain any amount of acid and methanol, such as phosphoric acid. Considering the speed, simplicity, and low cost, the dye stain may be of more practical value than other dye-based protein stains in routine proteomic research.http://europepmc.org/articles/PMC3151240?pdf=render |
| spellingShingle | Wei-Hua Dong Tian-Yun Wang Fang Wang Jun-He Zhang Simple, time-saving dye staining of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue. PLoS ONE |
| title | Simple, time-saving dye staining of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue. |
| title_full | Simple, time-saving dye staining of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue. |
| title_fullStr | Simple, time-saving dye staining of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue. |
| title_full_unstemmed | Simple, time-saving dye staining of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue. |
| title_short | Simple, time-saving dye staining of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue. |
| title_sort | simple time saving dye staining of proteins for sodium dodecyl sulfate polyacrylamide gel electrophoresis using coomassie blue |
| url | http://europepmc.org/articles/PMC3151240?pdf=render |
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