Ca-Alginate-PEGMA Hydrogels for In Situ Delivery of TGF-β Neutralizing Antibodies in a Mouse Model of Wound Healing
Hydrogels provide effective alternatives for drug delivery when therapeutics cannot be applied directly to a wound, or if adverse effects are associated with systemic administration. However, drug delivery vehicles need to be biocompatible and biodegradable and exhibit sufficient mechanical strength...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/3/1164 |
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| author | Jahaziel Gasperin-Bulbarela Ana B. Castro-Ceseña Tanya Camacho-Villegas Pavel H. Lugo-Fabres Nestor Emmanuel Díaz-Martínez Eduardo Padilla-Camberos Raquel Echavarría Alexei F. Licea-Navarro |
| author_facet | Jahaziel Gasperin-Bulbarela Ana B. Castro-Ceseña Tanya Camacho-Villegas Pavel H. Lugo-Fabres Nestor Emmanuel Díaz-Martínez Eduardo Padilla-Camberos Raquel Echavarría Alexei F. Licea-Navarro |
| author_sort | Jahaziel Gasperin-Bulbarela |
| collection | DOAJ |
| description | Hydrogels provide effective alternatives for drug delivery when therapeutics cannot be applied directly to a wound, or if adverse effects are associated with systemic administration. However, drug delivery vehicles need to be biocompatible and biodegradable and exhibit sufficient mechanical strength to withstand handling and different physiological conditions, such as those encountered during topical administration of a therapeutic. Wound healing can be divided into three phases stimulated by transforming growth factor-beta (TGF-β) and, subsequently, targeted therapeutics have been developed to inhibit this cytokine for the treatment of chronic wounds and to prevent scarring. In this study, the capacity of calcium alginate hydrogels plasticized with poly(ethylene glycol) methyl ether methacrylate (PEGMA) to deliver anti-TGF-β antibodies (1D11.16.8) to a wound was investigated in situ. Three levels of antibodies, 10, 50, and 100 μg, were loaded into calcium-alginate-PEGMA hydrogels and evaluated in an excisional wound model in mice. Hydrogels containing 50 and 100 μg 1D11.16.8 produced less inflammation, accompanied by a marked reduction in collagen deposition and cell infiltration. These findings demonstrate the capacity of calcium-alginate-PEGMA hydrogels to deliver larger proteins, such as antibodies, to the site of a wound. |
| first_indexed | 2024-03-09T03:32:59Z |
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| id | doaj.art-e7573d363e3a4e4b89a8a7126c5b7498 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T03:32:59Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-e7573d363e3a4e4b89a8a7126c5b74982023-12-03T14:53:42ZengMDPI AGApplied Sciences2076-34172021-01-01113116410.3390/app11031164Ca-Alginate-PEGMA Hydrogels for In Situ Delivery of TGF-β Neutralizing Antibodies in a Mouse Model of Wound HealingJahaziel Gasperin-Bulbarela0Ana B. Castro-Ceseña1Tanya Camacho-Villegas2Pavel H. Lugo-Fabres3Nestor Emmanuel Díaz-Martínez4Eduardo Padilla-Camberos5Raquel Echavarría6Alexei F. Licea-Navarro7Departamento de Innovación Biomédica, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana No. 3918, Zona Playitas, Ensenada C.P. 22860, Baja California, MexicoDepartamento de Innovación Biomédica, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana No. 3918, Zona Playitas, Ensenada C.P. 22860, Baja California, MexicoUnidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), A.C. Av. Normalistas 800 Colinas de la Normal, Guadalajara C.P. 44270, Jalisco, MexicoUnidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), A.C. Av. Normalistas 800 Colinas de la Normal, Guadalajara C.P. 44270, Jalisco, MexicoUnidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), A.C. Av. Normalistas 800 Colinas de la Normal, Guadalajara C.P. 44270, Jalisco, MexicoUnidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), A.C. Av. Normalistas 800 Colinas de la Normal, Guadalajara C.P. 44270, Jalisco, MexicoCONACyT-Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara C.P. 44340, Jalisco, MexicoDepartamento de Innovación Biomédica, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana No. 3918, Zona Playitas, Ensenada C.P. 22860, Baja California, MexicoHydrogels provide effective alternatives for drug delivery when therapeutics cannot be applied directly to a wound, or if adverse effects are associated with systemic administration. However, drug delivery vehicles need to be biocompatible and biodegradable and exhibit sufficient mechanical strength to withstand handling and different physiological conditions, such as those encountered during topical administration of a therapeutic. Wound healing can be divided into three phases stimulated by transforming growth factor-beta (TGF-β) and, subsequently, targeted therapeutics have been developed to inhibit this cytokine for the treatment of chronic wounds and to prevent scarring. In this study, the capacity of calcium alginate hydrogels plasticized with poly(ethylene glycol) methyl ether methacrylate (PEGMA) to deliver anti-TGF-β antibodies (1D11.16.8) to a wound was investigated in situ. Three levels of antibodies, 10, 50, and 100 μg, were loaded into calcium-alginate-PEGMA hydrogels and evaluated in an excisional wound model in mice. Hydrogels containing 50 and 100 μg 1D11.16.8 produced less inflammation, accompanied by a marked reduction in collagen deposition and cell infiltration. These findings demonstrate the capacity of calcium-alginate-PEGMA hydrogels to deliver larger proteins, such as antibodies, to the site of a wound.https://www.mdpi.com/2076-3417/11/3/1164hydrogelwound healingCa-alginatepoly(ethylene glycol) methyl ether methacrylate1D11TGF-β |
| spellingShingle | Jahaziel Gasperin-Bulbarela Ana B. Castro-Ceseña Tanya Camacho-Villegas Pavel H. Lugo-Fabres Nestor Emmanuel Díaz-Martínez Eduardo Padilla-Camberos Raquel Echavarría Alexei F. Licea-Navarro Ca-Alginate-PEGMA Hydrogels for In Situ Delivery of TGF-β Neutralizing Antibodies in a Mouse Model of Wound Healing Applied Sciences hydrogel wound healing Ca-alginate poly(ethylene glycol) methyl ether methacrylate 1D11 TGF-β |
| title | Ca-Alginate-PEGMA Hydrogels for In Situ Delivery of TGF-β Neutralizing Antibodies in a Mouse Model of Wound Healing |
| title_full | Ca-Alginate-PEGMA Hydrogels for In Situ Delivery of TGF-β Neutralizing Antibodies in a Mouse Model of Wound Healing |
| title_fullStr | Ca-Alginate-PEGMA Hydrogels for In Situ Delivery of TGF-β Neutralizing Antibodies in a Mouse Model of Wound Healing |
| title_full_unstemmed | Ca-Alginate-PEGMA Hydrogels for In Situ Delivery of TGF-β Neutralizing Antibodies in a Mouse Model of Wound Healing |
| title_short | Ca-Alginate-PEGMA Hydrogels for In Situ Delivery of TGF-β Neutralizing Antibodies in a Mouse Model of Wound Healing |
| title_sort | ca alginate pegma hydrogels for in situ delivery of tgf β neutralizing antibodies in a mouse model of wound healing |
| topic | hydrogel wound healing Ca-alginate poly(ethylene glycol) methyl ether methacrylate 1D11 TGF-β |
| url | https://www.mdpi.com/2076-3417/11/3/1164 |
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