Reproducibility of extracellular vesicle research
Cells release membrane-delimited particles into the environment. These particles are called “extracellular vesicles” (EVs), and EVs are present in fluids contacting cells, including body fluids and conditioned culture media. Because EVs change and contribute to health and disease, EVs have become a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | European Journal of Cell Biology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171933522000292 |
| _version_ | 1811313501137797120 |
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| author | Rienk Nieuwland Pia R.-M. Siljander Juan M. Falcón-Pérez Kenneth W. Witwer |
| author_facet | Rienk Nieuwland Pia R.-M. Siljander Juan M. Falcón-Pérez Kenneth W. Witwer |
| author_sort | Rienk Nieuwland |
| collection | DOAJ |
| description | Cells release membrane-delimited particles into the environment. These particles are called “extracellular vesicles” (EVs), and EVs are present in fluids contacting cells, including body fluids and conditioned culture media. Because EVs change and contribute to health and disease, EVs have become a hot topic. From the thousands of papers now published on EVs annually, one easily gets the impression that EVs provide biomarkers for all diseases, and that EVs are carriers of all relevant biomolecules and are omnipotent therapeutics. At the same time, EVs are heterogeneous, elusive and difficult to study due to their physical properties and the complex composition of their environment. This overview addresses the current challenges encountered when working with EVs, and how we envision that most of these challenges will be overcome in the near future. Right now, an infrastructure is being developed to improve the reproducibility of EV measurement results. This infrastructure comprises expert task forces of the International Society of Extracellular Vesicles (ISEV) developing guidelines and recommendations, instrument calibration, standardized and transparent reporting, and education. Altogether, these developments will support the credibility of EV research by introducing robust reproducibility, which is a prerequisite for understanding their biological significance and biomarker potential. |
| first_indexed | 2024-04-13T10:55:03Z |
| format | Article |
| id | doaj.art-e75bc711b64245c1bbe1baad364c520d |
| institution | Directory Open Access Journal |
| issn | 0171-9335 |
| language | English |
| last_indexed | 2024-04-13T10:55:03Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | European Journal of Cell Biology |
| spelling | doaj.art-e75bc711b64245c1bbe1baad364c520d2022-12-22T02:49:33ZengElsevierEuropean Journal of Cell Biology0171-93352022-06-011013151226Reproducibility of extracellular vesicle researchRienk Nieuwland0Pia R.-M. Siljander1Juan M. Falcón-Pérez2Kenneth W. Witwer3Laboratory of Experimental Clinical Chemistry, Department of Clinical Chemistry, Amsterdam UMC location University of Amsterdam, Meibergdreef 9, Amsterdam, The Netherlands; Vesicle Observation Centre, Amsterdam UMC location University of Amsterdam, Meibergdreef 9, Amsterdam, The Netherlands; Corresponding author at: Laboratory of Experimental Clinical Chemistry, Department of Clinical Chemistry, Amsterdam UMC location University of Amsterdam, Meibergdreef 9, Amsterdam, The Netherlands.EV Group, Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, FinlandCenter for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Exosomes Laboratory, Derio, Spain; Centro de Investigación Biomédica en Red de enfermedades hepáticas y digestivas (CIBERehd), 28029 Madrid, Spain; IKERBASQUE, Basque Foundation for Science, 48013 Bilbao, Spain; Corresponding author at: Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Exosomes Laboratory, Derio, Spain.Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Corresponding author at: Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.Cells release membrane-delimited particles into the environment. These particles are called “extracellular vesicles” (EVs), and EVs are present in fluids contacting cells, including body fluids and conditioned culture media. Because EVs change and contribute to health and disease, EVs have become a hot topic. From the thousands of papers now published on EVs annually, one easily gets the impression that EVs provide biomarkers for all diseases, and that EVs are carriers of all relevant biomolecules and are omnipotent therapeutics. At the same time, EVs are heterogeneous, elusive and difficult to study due to their physical properties and the complex composition of their environment. This overview addresses the current challenges encountered when working with EVs, and how we envision that most of these challenges will be overcome in the near future. Right now, an infrastructure is being developed to improve the reproducibility of EV measurement results. This infrastructure comprises expert task forces of the International Society of Extracellular Vesicles (ISEV) developing guidelines and recommendations, instrument calibration, standardized and transparent reporting, and education. Altogether, these developments will support the credibility of EV research by introducing robust reproducibility, which is a prerequisite for understanding their biological significance and biomarker potential.http://www.sciencedirect.com/science/article/pii/S0171933522000292 |
| spellingShingle | Rienk Nieuwland Pia R.-M. Siljander Juan M. Falcón-Pérez Kenneth W. Witwer Reproducibility of extracellular vesicle research European Journal of Cell Biology |
| title | Reproducibility of extracellular vesicle research |
| title_full | Reproducibility of extracellular vesicle research |
| title_fullStr | Reproducibility of extracellular vesicle research |
| title_full_unstemmed | Reproducibility of extracellular vesicle research |
| title_short | Reproducibility of extracellular vesicle research |
| title_sort | reproducibility of extracellular vesicle research |
| url | http://www.sciencedirect.com/science/article/pii/S0171933522000292 |
| work_keys_str_mv | AT rienknieuwland reproducibilityofextracellularvesicleresearch AT piarmsiljander reproducibilityofextracellularvesicleresearch AT juanmfalconperez reproducibilityofextracellularvesicleresearch AT kennethwwitwer reproducibilityofextracellularvesicleresearch |