Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates
Background and Purpose: Candida albicans is the most common Candida species (spp.) isolated from fungal infections. Azole resistance in Candida species has been considerably increased in the last decades. Given the toxicity of the antimicrobial drugs, resistance to antifungal agents, and drug intera...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2017-09-01
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| Series: | Current Medical Mycology |
| Subjects: | |
| Online Access: | http://cmm.mazums.ac.ir/browse.php?a_code=A-10-87-3&slc_lang=en&sid=1 |
| _version_ | 1811302340332879872 |
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| author | Nasrin Parsamehr Sasan Rezaie Sadegh Khodavaisy Samira Salari Sanaz Hadizadeh Mohammad Kord Seyed Amin Ayatollahi Mousavi |
| author_facet | Nasrin Parsamehr Sasan Rezaie Sadegh Khodavaisy Samira Salari Sanaz Hadizadeh Mohammad Kord Seyed Amin Ayatollahi Mousavi |
| author_sort | Nasrin Parsamehr |
| collection | DOAJ |
| description | Background and Purpose: Candida albicans is the most common Candida species (spp.) isolated from fungal infections. Azole resistance in Candida species has been considerably increased in the last decades. Given the toxicity of the antimicrobial drugs, resistance to antifungal agents, and drug interactions, the identification of new antifungal agents seems essential. In this study, we assessed the antifungal effects of biogenic selenium nanoparticles on C. albicans and determined the expression of ERG11 and CDR1 genes.
Materials and Methods: Selenium nanoparticles were synthesized with Bacillus sp. MSH-1. The ultrastructure of selenium nanoparticles was evaluated with a transmission electron microscope. The antifungal susceptibility test was performed according to the modified Clinical and Laboratory Standards Institute M27-A3 standard protocol. The expression levels of the CDR1 and ERG11 genes were analyzed using the quantitative real-time polymerase chain reaction (PCR) assay.
Results: The azole-resistant C. albicans and wild type C. albicans strains were inhibited by 100 and 70 µg/mL of selenium nanoparticle concentrations, respectively. The expression of CDR1 and ERG11 genes was significantly down-regulated in these selenium nanoparticle concentrations.
Conclusion: As the findings indicated, selenium nanoparticles had an appropriate antifungal activity against fluconazole-resistant and -susceptible C. albicans strains. Accordingly, these nanoparticles reduced the expression of CDR1 and ERG11 genes associated with azole resistance. Further studies are needed to investigate the synergistic effects of selenium nanoparticles using other antifungal drugs.
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| first_indexed | 2024-04-13T07:26:18Z |
| format | Article |
| id | doaj.art-e75e0b5f03854a4d812160bd33eedc80 |
| institution | Directory Open Access Journal |
| issn | 2423-3439 2423-3420 |
| language | English |
| last_indexed | 2024-04-13T07:26:18Z |
| publishDate | 2017-09-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Current Medical Mycology |
| spelling | doaj.art-e75e0b5f03854a4d812160bd33eedc802022-12-22T02:56:28ZengMazandaran University of Medical SciencesCurrent Medical Mycology2423-34392423-34202017-09-01331620Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolatesNasrin Parsamehr0Sasan Rezaie1Sadegh Khodavaisy2Samira Salari3Sanaz Hadizadeh4Mohammad Kord5Seyed Amin Ayatollahi Mousavi6 1Department of Medical Parasitology and Mycology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 1Department of Medical Parasitology and Mycology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran 1Department of Medical Parasitology and Mycology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 1Department of Medical Parasitology and Mycology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran Background and Purpose: Candida albicans is the most common Candida species (spp.) isolated from fungal infections. Azole resistance in Candida species has been considerably increased in the last decades. Given the toxicity of the antimicrobial drugs, resistance to antifungal agents, and drug interactions, the identification of new antifungal agents seems essential. In this study, we assessed the antifungal effects of biogenic selenium nanoparticles on C. albicans and determined the expression of ERG11 and CDR1 genes. Materials and Methods: Selenium nanoparticles were synthesized with Bacillus sp. MSH-1. The ultrastructure of selenium nanoparticles was evaluated with a transmission electron microscope. The antifungal susceptibility test was performed according to the modified Clinical and Laboratory Standards Institute M27-A3 standard protocol. The expression levels of the CDR1 and ERG11 genes were analyzed using the quantitative real-time polymerase chain reaction (PCR) assay. Results: The azole-resistant C. albicans and wild type C. albicans strains were inhibited by 100 and 70 µg/mL of selenium nanoparticle concentrations, respectively. The expression of CDR1 and ERG11 genes was significantly down-regulated in these selenium nanoparticle concentrations. Conclusion: As the findings indicated, selenium nanoparticles had an appropriate antifungal activity against fluconazole-resistant and -susceptible C. albicans strains. Accordingly, these nanoparticles reduced the expression of CDR1 and ERG11 genes associated with azole resistance. Further studies are needed to investigate the synergistic effects of selenium nanoparticles using other antifungal drugs. http://cmm.mazums.ac.ir/browse.php?a_code=A-10-87-3&slc_lang=en&sid=1Candida albicans Nanoparticles ERG 11 CDR1 |
| spellingShingle | Nasrin Parsamehr Sasan Rezaie Sadegh Khodavaisy Samira Salari Sanaz Hadizadeh Mohammad Kord Seyed Amin Ayatollahi Mousavi Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates Current Medical Mycology Candida albicans Nanoparticles ERG 11 CDR1 |
| title | Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates |
| title_full | Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates |
| title_fullStr | Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates |
| title_full_unstemmed | Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates |
| title_short | Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates |
| title_sort | effect of biogenic selenium nanoparticles on erg11 and cdr1 gene expression in both fluconazole resistant and susceptible candida albicans isolates |
| topic | Candida albicans Nanoparticles ERG 11 CDR1 |
| url | http://cmm.mazums.ac.ir/browse.php?a_code=A-10-87-3&slc_lang=en&sid=1 |
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