CRISPR/Cas12a Coupled With Recombinase Polymerase Amplification for Sensitive and Specific Detection of Aphelenchoides besseyi

CRISPR/Cas12a Coupled With Recombinase Polymerase Amplification for Sensitive and Specific Detection of Aphelenchoides besseyi

Aphelenchoides besseyi (A. besseyi), a seed-borne parasitic nematode, is the causal agent of rice white tip disease (RWTD), which may result in a drastic loss of rice yield. Seed treatments are currently considered to be the most effective means of preventing the spread of RWTD. Therefore, the rapid...

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Bibliographic Details
Main Authors: Anpeng Zhang, Bin Sun, Jianming Zhang, Can Cheng, Jihua Zhou, Fuan Niu, Zhongyong Luo, Luzhen Yu, Cui Yu, Yuting Dai, Kaizhen Xie, Qiyan Hu, Yue Qiu, Liming Cao, Huangwei Chu
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-06-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
CRISPR/Cas12a
RPA
Aphelenchoides besseyi
rice
molecular identification
detection
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2022.912959/full

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