Molecular mechanism of anti-obesity effect of total lutein oxidized products (LOPs) in diet-induced obese mice
The present study explores the molecular mechanism of anti-obesity effect of total lutein oxidized products (LOPs) in high-fat diet-induced obese C57BL/6 mice. Total LOPs (50, 100, 200 mg/kg b.wt.) were intubated for 22 weeks. Lipid profile, peroxides, AST, ALT, GST activities, lipid metabolizing en...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Taibah University for Science |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16583655.2023.2226489 |
| _version_ | 1827288161857830912 |
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| author | Nagashree Shamarao Mukunda Chethankumar |
| author_facet | Nagashree Shamarao Mukunda Chethankumar |
| author_sort | Nagashree Shamarao |
| collection | DOAJ |
| description | The present study explores the molecular mechanism of anti-obesity effect of total lutein oxidized products (LOPs) in high-fat diet-induced obese C57BL/6 mice. Total LOPs (50, 100, 200 mg/kg b.wt.) were intubated for 22 weeks. Lipid profile, peroxides, AST, ALT, GST activities, lipid metabolizing enzymes/molecules, 3T3-L1 cell differentiation, TGF-β1 and p38 MAPK expressions were studied. Total LOPs at 100 mg/kg b.wt. reduced plasma and hepatic cholesterol, triglycerides, phospholipids, ALT and AST activities. FAS and CPT activities were inhibited in the liver homogenate, and epididymal WAT (eWAT). Total LOPs at 200 mg/kg b.wt. reduced HMGCoA:Mevalonate in eWAT, lipid peroxides in eWAT and liver. GST activity was high in the liver and eWAT. Total LOPs (100μg/ml) suppressed adipogenesis and decreased the accumulation of lipid droplets from two to eight days. Adiponectin was upregulated, PPAR γ, lipoprotein lipase, TGF-β1, and p38 MAPK were down-regulated. The results have shown that total LOPs are potent anti-obese molecules.ABBREVIATIONS: AIN 93G: American institute of nutrition 93 for growth; ALT: alanine transaminase; AST: aspartate transaminase; CEBPα: ccaat/enhancer binding protein α; C-DNA: complementary deoxyribonucleic acid; CPT: carnitine palmitoyl transferase; GST: glutathione-S-transferase; ELISA: enzyme-linked immune sorbent assay; FAS: fatty acid synthase; HDL: high-density lipoprotein; HFD: high-fat diet; HMG CoA: β-Hydroxy β-methylglutaryl-Coenzyme A; LDL: low-density lipoprotein; LOPs: total lutein oxidized products; LPL: lipoprotein lipase; MDA-Malonaldehyde; MTT: 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyl tetrazolium bromide; MVA: mevalonate; p38MAPK: p38 mitogen-activated protein kinases; RNA: ribonucleic acid; RTq-PCR: quantitative reverse transcription polymerase chain reaction; PPAR-γ: peroxisome proliferator-activated receptor-gamma; TGF-β1: transforming growth factor beta 1; WAT: white adipose tissue. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1658-3655 |
| language | English |
| last_indexed | 2024-04-24T11:26:10Z |
| publishDate | 2023-12-01 |
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| spelling | doaj.art-e75fdd92837e4da2bae7c81d9d2a52102024-04-10T20:17:48ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552023-12-0117110.1080/16583655.2023.2226489Molecular mechanism of anti-obesity effect of total lutein oxidized products (LOPs) in diet-induced obese miceNagashree Shamarao0Mukunda Chethankumar1JSS Research Foundation, SJCE Technical Institutions Campus, Mysuru, Karnataka, IndiaDepartment of Biochemistry, JSS College of Arts Commerce and Science (Autonomous), Mysuru, Karnataka, IndiaThe present study explores the molecular mechanism of anti-obesity effect of total lutein oxidized products (LOPs) in high-fat diet-induced obese C57BL/6 mice. Total LOPs (50, 100, 200 mg/kg b.wt.) were intubated for 22 weeks. Lipid profile, peroxides, AST, ALT, GST activities, lipid metabolizing enzymes/molecules, 3T3-L1 cell differentiation, TGF-β1 and p38 MAPK expressions were studied. Total LOPs at 100 mg/kg b.wt. reduced plasma and hepatic cholesterol, triglycerides, phospholipids, ALT and AST activities. FAS and CPT activities were inhibited in the liver homogenate, and epididymal WAT (eWAT). Total LOPs at 200 mg/kg b.wt. reduced HMGCoA:Mevalonate in eWAT, lipid peroxides in eWAT and liver. GST activity was high in the liver and eWAT. Total LOPs (100μg/ml) suppressed adipogenesis and decreased the accumulation of lipid droplets from two to eight days. Adiponectin was upregulated, PPAR γ, lipoprotein lipase, TGF-β1, and p38 MAPK were down-regulated. The results have shown that total LOPs are potent anti-obese molecules.ABBREVIATIONS: AIN 93G: American institute of nutrition 93 for growth; ALT: alanine transaminase; AST: aspartate transaminase; CEBPα: ccaat/enhancer binding protein α; C-DNA: complementary deoxyribonucleic acid; CPT: carnitine palmitoyl transferase; GST: glutathione-S-transferase; ELISA: enzyme-linked immune sorbent assay; FAS: fatty acid synthase; HDL: high-density lipoprotein; HFD: high-fat diet; HMG CoA: β-Hydroxy β-methylglutaryl-Coenzyme A; LDL: low-density lipoprotein; LOPs: total lutein oxidized products; LPL: lipoprotein lipase; MDA-Malonaldehyde; MTT: 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyl tetrazolium bromide; MVA: mevalonate; p38MAPK: p38 mitogen-activated protein kinases; RNA: ribonucleic acid; RTq-PCR: quantitative reverse transcription polymerase chain reaction; PPAR-γ: peroxisome proliferator-activated receptor-gamma; TGF-β1: transforming growth factor beta 1; WAT: white adipose tissue.https://www.tandfonline.com/doi/10.1080/16583655.2023.2226489Lutein oxidized productsC57BL/6 micelipid metabolizing enzymes3T3-L1 cellsadiponectinp38 MAPK |
| spellingShingle | Nagashree Shamarao Mukunda Chethankumar Molecular mechanism of anti-obesity effect of total lutein oxidized products (LOPs) in diet-induced obese mice Journal of Taibah University for Science Lutein oxidized products C57BL/6 mice lipid metabolizing enzymes 3T3-L1 cells adiponectin p38 MAPK |
| title | Molecular mechanism of anti-obesity effect of total lutein oxidized products (LOPs) in diet-induced obese mice |
| title_full | Molecular mechanism of anti-obesity effect of total lutein oxidized products (LOPs) in diet-induced obese mice |
| title_fullStr | Molecular mechanism of anti-obesity effect of total lutein oxidized products (LOPs) in diet-induced obese mice |
| title_full_unstemmed | Molecular mechanism of anti-obesity effect of total lutein oxidized products (LOPs) in diet-induced obese mice |
| title_short | Molecular mechanism of anti-obesity effect of total lutein oxidized products (LOPs) in diet-induced obese mice |
| title_sort | molecular mechanism of anti obesity effect of total lutein oxidized products lops in diet induced obese mice |
| topic | Lutein oxidized products C57BL/6 mice lipid metabolizing enzymes 3T3-L1 cells adiponectin p38 MAPK |
| url | https://www.tandfonline.com/doi/10.1080/16583655.2023.2226489 |
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