Protective efficacy of inactivated Newcastle disease virus vaccines prepared in two different oil-based adjuvants
Despite the availability of Newcastle disease (ND) vaccines for more than six decades, disease outbreaks continue to occur with huge economic consequences to the global poultry industry. The aim of this study is to develop a safe and effective inactivated vaccine based on a recently isolated Newcast...
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| Format: | Article |
| Language: | English |
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AOSIS
2020-09-01
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| Series: | Onderstepoort Journal of Veterinary Research |
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| Online Access: | https://ojvr.org/index.php/ojvr/article/view/1865 |
| _version_ | 1831840381079126016 |
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| author | Oday A. Aljumaili Muhammad B. Bello Swee K. Yeap Abdul R. Omar Aini Ideris |
| author_facet | Oday A. Aljumaili Muhammad B. Bello Swee K. Yeap Abdul R. Omar Aini Ideris |
| author_sort | Oday A. Aljumaili |
| collection | DOAJ |
| description | Despite the availability of Newcastle disease (ND) vaccines for more than six decades, disease outbreaks continue to occur with huge economic consequences to the global poultry industry. The aim of this study is to develop a safe and effective inactivated vaccine based on a recently isolated Newcastle disease virus (NDV) strain IBS025/13 and evaluate its protective efficacy in chicken following challenge with a highly virulent genotype VII isolate. Firstly, high titre of IBS025/13 was exposed to various concentrations of binary ethylenimine (BEI) to determine the optimal conditions for complete inactivation of the virus. The inactivated virus was then prepared in form of a stable water-in-oil emulsion of black seed oil (BSO) or Freund’s incomplete adjuvant (FIA) and used as vaccines in specific pathogen-free chicken. Efficacy of various vaccine preparations was also evaluated based on the ability of the vaccine to protect against clinical disease, mortality and virus shedding following challenge with highly virulent genotype\VII NDV isolate. The results indicate that exposure of NDV IBS025/13 to 10 mM of BEI for 21 h at 37 °C could completely inactivate the virus without tempering with the structural integrity of the viral hemagglutin-neuraminidase protein. More so, the inactivated vaccines adjuvanted with either BSO- or FIA-induced high hemagglutination inhibition antibody titre that protected the vaccinated birds against clinical disease and in some cases virus shedding, especially when used together with live attenuated vaccines. Thus, genotype VII-based NDV-inactivated vaccines formulated in BSO could substantially improve poultry disease control particularly when combined with live attenuated vaccines. |
| first_indexed | 2024-12-23T06:10:48Z |
| format | Article |
| id | doaj.art-e76891ee1b984f1ca0b3eb3cd61dd78f |
| institution | Directory Open Access Journal |
| issn | 0030-2465 2219-0635 |
| language | English |
| last_indexed | 2024-12-23T06:10:48Z |
| publishDate | 2020-09-01 |
| publisher | AOSIS |
| record_format | Article |
| series | Onderstepoort Journal of Veterinary Research |
| spelling | doaj.art-e76891ee1b984f1ca0b3eb3cd61dd78f2022-12-21T17:57:28ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352020-09-01871e1e710.4102/ojvr.v87i1.1865642Protective efficacy of inactivated Newcastle disease virus vaccines prepared in two different oil-based adjuvantsOday A. Aljumaili0Muhammad B. Bello1Swee K. Yeap2Abdul R. Omar3Aini Ideris4Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Malaysia; and, Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, SerdangLaboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia; and, Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, SokotoLaboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, SerdangLaboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia; and, Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, SerdangDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Malaysia; and, Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, SerdangDespite the availability of Newcastle disease (ND) vaccines for more than six decades, disease outbreaks continue to occur with huge economic consequences to the global poultry industry. The aim of this study is to develop a safe and effective inactivated vaccine based on a recently isolated Newcastle disease virus (NDV) strain IBS025/13 and evaluate its protective efficacy in chicken following challenge with a highly virulent genotype VII isolate. Firstly, high titre of IBS025/13 was exposed to various concentrations of binary ethylenimine (BEI) to determine the optimal conditions for complete inactivation of the virus. The inactivated virus was then prepared in form of a stable water-in-oil emulsion of black seed oil (BSO) or Freund’s incomplete adjuvant (FIA) and used as vaccines in specific pathogen-free chicken. Efficacy of various vaccine preparations was also evaluated based on the ability of the vaccine to protect against clinical disease, mortality and virus shedding following challenge with highly virulent genotype\VII NDV isolate. The results indicate that exposure of NDV IBS025/13 to 10 mM of BEI for 21 h at 37 °C could completely inactivate the virus without tempering with the structural integrity of the viral hemagglutin-neuraminidase protein. More so, the inactivated vaccines adjuvanted with either BSO- or FIA-induced high hemagglutination inhibition antibody titre that protected the vaccinated birds against clinical disease and in some cases virus shedding, especially when used together with live attenuated vaccines. Thus, genotype VII-based NDV-inactivated vaccines formulated in BSO could substantially improve poultry disease control particularly when combined with live attenuated vaccines.https://ojvr.org/index.php/ojvr/article/view/1865newcastle disease virusgenotype viiinactivated vaccinewater-in-oil emulsionvirus shedding |
| spellingShingle | Oday A. Aljumaili Muhammad B. Bello Swee K. Yeap Abdul R. Omar Aini Ideris Protective efficacy of inactivated Newcastle disease virus vaccines prepared in two different oil-based adjuvants Onderstepoort Journal of Veterinary Research newcastle disease virus genotype vii inactivated vaccine water-in-oil emulsion virus shedding |
| title | Protective efficacy of inactivated Newcastle disease virus vaccines prepared in two different oil-based adjuvants |
| title_full | Protective efficacy of inactivated Newcastle disease virus vaccines prepared in two different oil-based adjuvants |
| title_fullStr | Protective efficacy of inactivated Newcastle disease virus vaccines prepared in two different oil-based adjuvants |
| title_full_unstemmed | Protective efficacy of inactivated Newcastle disease virus vaccines prepared in two different oil-based adjuvants |
| title_short | Protective efficacy of inactivated Newcastle disease virus vaccines prepared in two different oil-based adjuvants |
| title_sort | protective efficacy of inactivated newcastle disease virus vaccines prepared in two different oil based adjuvants |
| topic | newcastle disease virus genotype vii inactivated vaccine water-in-oil emulsion virus shedding |
| url | https://ojvr.org/index.php/ojvr/article/view/1865 |
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