Modelling cell shape in 3D structured environments: A quantitative comparison with experiments.
Cell shape plays a fundamental role in many biological processes, including adhesion, migration, division and development, but it is not clear which shape model best predicts three-dimensional cell shape in structured environments. Here, we compare different modelling approaches with experimental da...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-04-01
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| Series: | PLoS Computational Biology |
| Online Access: | https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1011412&type=printable |
| _version_ | 1797200764002631680 |
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| author | Rabea Link Mona Jaggy Martin Bastmeyer Ulrich S Schwarz |
| author_facet | Rabea Link Mona Jaggy Martin Bastmeyer Ulrich S Schwarz |
| author_sort | Rabea Link |
| collection | DOAJ |
| description | Cell shape plays a fundamental role in many biological processes, including adhesion, migration, division and development, but it is not clear which shape model best predicts three-dimensional cell shape in structured environments. Here, we compare different modelling approaches with experimental data. The shapes of single mesenchymal cells cultured in custom-made 3D scaffolds were compared by a Fourier method with surfaces that minimize area under the given adhesion and volume constraints. For the minimized surface model, we found marked differences to the experimentally observed cell shapes, which necessitated the use of more advanced shape models. We used different variants of the cellular Potts model, which effectively includes both surface and bulk contributions. The simulations revealed that the Hamiltonian with linear area energy outperformed the elastic area constraint in accurately modelling the 3D shapes of cells in structured environments. Explicit modelling the nucleus did not improve the accuracy of the simulated cell shapes. Overall, our work identifies effective methods for accurately modelling cellular shapes in complex environments. |
| first_indexed | 2024-04-24T07:36:50Z |
| format | Article |
| id | doaj.art-e76cbef440cd412a8dd3bbd294167908 |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-04-24T07:36:50Z |
| publishDate | 2024-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-e76cbef440cd412a8dd3bbd2941679082024-04-20T05:31:10ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582024-04-01204e101141210.1371/journal.pcbi.1011412Modelling cell shape in 3D structured environments: A quantitative comparison with experiments.Rabea LinkMona JaggyMartin BastmeyerUlrich S SchwarzCell shape plays a fundamental role in many biological processes, including adhesion, migration, division and development, but it is not clear which shape model best predicts three-dimensional cell shape in structured environments. Here, we compare different modelling approaches with experimental data. The shapes of single mesenchymal cells cultured in custom-made 3D scaffolds were compared by a Fourier method with surfaces that minimize area under the given adhesion and volume constraints. For the minimized surface model, we found marked differences to the experimentally observed cell shapes, which necessitated the use of more advanced shape models. We used different variants of the cellular Potts model, which effectively includes both surface and bulk contributions. The simulations revealed that the Hamiltonian with linear area energy outperformed the elastic area constraint in accurately modelling the 3D shapes of cells in structured environments. Explicit modelling the nucleus did not improve the accuracy of the simulated cell shapes. Overall, our work identifies effective methods for accurately modelling cellular shapes in complex environments.https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1011412&type=printable |
| spellingShingle | Rabea Link Mona Jaggy Martin Bastmeyer Ulrich S Schwarz Modelling cell shape in 3D structured environments: A quantitative comparison with experiments. PLoS Computational Biology |
| title | Modelling cell shape in 3D structured environments: A quantitative comparison with experiments. |
| title_full | Modelling cell shape in 3D structured environments: A quantitative comparison with experiments. |
| title_fullStr | Modelling cell shape in 3D structured environments: A quantitative comparison with experiments. |
| title_full_unstemmed | Modelling cell shape in 3D structured environments: A quantitative comparison with experiments. |
| title_short | Modelling cell shape in 3D structured environments: A quantitative comparison with experiments. |
| title_sort | modelling cell shape in 3d structured environments a quantitative comparison with experiments |
| url | https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1011412&type=printable |
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