Testing of apathogenic influenza virus H5N3 as a poultry live vaccine
Four H5N2 experimental vaccine strains and the apathogenic wild duck H5N3 influenza virus A/duck/ Moscow/4182/2010 (dk/4182) were tested as a live poultry vaccine. Experimental strains had the hemagglutinin of the A/Vietnam/1203/04 strain lacking the polybasic HA cleavage site or the hemagglutinin f...
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| Format: | Article |
| Language: | English |
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Central Research Institute for Epidemiology
2015-08-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/345/223 |
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| author | E. Y. Boravleva I. A. Chvala N. F. Lomakina P. I. Repin N. S. Mudrak L. G. Rudenko A. S. Gambaryan V. V. Drygin |
| author_facet | E. Y. Boravleva I. A. Chvala N. F. Lomakina P. I. Repin N. S. Mudrak L. G. Rudenko A. S. Gambaryan V. V. Drygin |
| author_sort | E. Y. Boravleva |
| collection | DOAJ |
| description | Four H5N2 experimental vaccine strains and the apathogenic wild duck H5N3 influenza virus A/duck/ Moscow/4182/2010 (dk/4182) were tested as a live poultry vaccine. Experimental strains had the hemagglutinin of the A/Vietnam/1203/04 strain lacking the polybasic HA cleavage site or the hemagglutinin from attenuated virus (Ku/at) that was derived from the highly pathogenic influenza virus A/chicken/Kurgan/3/2005 (H5N1). The hemagglutinin of the Ku-at has the amino acid substitutions Asp54/Asn and Lys222/Thr in HA1 and Val48/Ile and Lys131/Thr in HA2, while maintaining the polybasic HA cleavage site at an invariable level. The other genes of these experimental strains were from the H2N2 cold-adapted master strain A/Leningrad/134/17/57 (VN-Len and Ku-Len) or from the apathogenic H6N2 virus A/gull/Moscow/3100/2006 (VN-Gull and Ku-Gull). A single immunization of mice with all tested strains elicited a high level of serum antibodies and provided complete protection against the challenge with the lethal dose of A/chicken/Kurgan/3/05. The pathogenicity indexes of the Ku-at and the other strains for chicken were virtually zero, whereas the index of the parent H5N1 virus A/chicken/Kurgan/3/2005 was 2.98. Intravenous, intranasal, and aerosol routes of vaccination were compared. It was shown that the strain dk/4182 was totally apathogenic for one-day-old chicken and provided complete protection against the highly pathogenic H5N1 virus. |
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| id | doaj.art-e76e2ebc884a476d9945367cb2c48f59 |
| institution | Directory Open Access Journal |
| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-13T00:08:12Z |
| publishDate | 2015-08-01 |
| publisher | Central Research Institute for Epidemiology |
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| series | Вопросы вирусологии |
| spelling | doaj.art-e76e2ebc884a476d9945367cb2c48f592023-07-12T20:15:03ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972015-08-016044449299Testing of apathogenic influenza virus H5N3 as a poultry live vaccineE. Y. Boravleva0I. A. Chvala1N. F. Lomakina2P. I. Repin3N. S. Mudrak4L. G. Rudenko5A. S. Gambaryan6V. V. Drygin7M.P. Chumakov Institute of Poliomyelitis and Viral EncephalitidesFederal Center for Animal Health (FGBI “ARRIAH”)M.P. Chumakov Institute of Poliomyelitis and Viral EncephalitidesFederal Center for Animal Health (FGBI “ARRIAH”)Institute of Experimental MedicineInstitute of Experimental MedicineM.P. Chumakov Institute of Poliomyelitis and Viral EncephalitidesFederal Center for Animal Health (FGBI “ARRIAH”)Four H5N2 experimental vaccine strains and the apathogenic wild duck H5N3 influenza virus A/duck/ Moscow/4182/2010 (dk/4182) were tested as a live poultry vaccine. Experimental strains had the hemagglutinin of the A/Vietnam/1203/04 strain lacking the polybasic HA cleavage site or the hemagglutinin from attenuated virus (Ku/at) that was derived from the highly pathogenic influenza virus A/chicken/Kurgan/3/2005 (H5N1). The hemagglutinin of the Ku-at has the amino acid substitutions Asp54/Asn and Lys222/Thr in HA1 and Val48/Ile and Lys131/Thr in HA2, while maintaining the polybasic HA cleavage site at an invariable level. The other genes of these experimental strains were from the H2N2 cold-adapted master strain A/Leningrad/134/17/57 (VN-Len and Ku-Len) or from the apathogenic H6N2 virus A/gull/Moscow/3100/2006 (VN-Gull and Ku-Gull). A single immunization of mice with all tested strains elicited a high level of serum antibodies and provided complete protection against the challenge with the lethal dose of A/chicken/Kurgan/3/05. The pathogenicity indexes of the Ku-at and the other strains for chicken were virtually zero, whereas the index of the parent H5N1 virus A/chicken/Kurgan/3/2005 was 2.98. Intravenous, intranasal, and aerosol routes of vaccination were compared. It was shown that the strain dk/4182 was totally apathogenic for one-day-old chicken and provided complete protection against the highly pathogenic H5N1 virus.https://virusjour.crie.ru/jour/article/viewFile/345/223influenza virus ah5n1poultry vaccine |
| spellingShingle | E. Y. Boravleva I. A. Chvala N. F. Lomakina P. I. Repin N. S. Mudrak L. G. Rudenko A. S. Gambaryan V. V. Drygin Testing of apathogenic influenza virus H5N3 as a poultry live vaccine Вопросы вирусологии influenza virus a h5n1 poultry vaccine |
| title | Testing of apathogenic influenza virus H5N3 as a poultry live vaccine |
| title_full | Testing of apathogenic influenza virus H5N3 as a poultry live vaccine |
| title_fullStr | Testing of apathogenic influenza virus H5N3 as a poultry live vaccine |
| title_full_unstemmed | Testing of apathogenic influenza virus H5N3 as a poultry live vaccine |
| title_short | Testing of apathogenic influenza virus H5N3 as a poultry live vaccine |
| title_sort | testing of apathogenic influenza virus h5n3 as a poultry live vaccine |
| topic | influenza virus a h5n1 poultry vaccine |
| url | https://virusjour.crie.ru/jour/article/viewFile/345/223 |
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