A preliminary molecular study of the Iranian species of Calomyscus (Rodentia- Calomyscidae) using RFLP

The Calomyscidae is a small family of Rodentia that has recently been classified into 8 species in a single genus, Calomyscus. Currently 5 species are reported in Iran: C. elburzensis,، C. bailwardi، C. grandis ، C. urartensis and C. hotsoni. Species discrimination based on morphological characteris...

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Bibliographic Details
Main Authors: B. Sahebjam, J. Darvish, E. Rastegar-Pouyani, R. Siahsarvie, S. Akbarirad
Format: Article
Language:English
Published: Ferdowsi University of Mashhad 2009-07-01
Series:Iranian Journal of Animal Biosystematics
Subjects:
Online Access:https://ijab.um.ac.ir/article_25141_76761a14db3856740284750da1bc87b4.pdf
Description
Summary:The Calomyscidae is a small family of Rodentia that has recently been classified into 8 species in a single genus, Calomyscus. Currently 5 species are reported in Iran: C. elburzensis,، C. bailwardi، C. grandis ، C. urartensis and C. hotsoni. Species discrimination based on morphological characteristics is difficult, so this study re-evaluated the phylogenetic relationships and taxonomic status of the morphotypes within Calomyscus using PCRRFLP as a molecular marker. Forty-seven samples of Calomyscus spp from 9 geographically separated regions of Iran were analyzed. The cytochrome b gene was amplified by PCR, digested using 3 restriction enzymes, HinfI, HincII and HaeIII, and the patterns separated electrophoretically. Species of long tailed hamster were discriminated from one another. A phylogenetic analysis of the dataset produced well distinguished clades.
ISSN:1735-434X
2423-4222