Distribution of <i>Corynebacterium</i> Species and Comparative Results of Diagnostic Methods for Identifying <i>Corynebacterium</i> in Experimental Mice in Korea
The genus <i>Corynebacterium</i>, composed of Gram-positive diphtheroid rod-shaped bacteria, induces severe diseases, such as <i>Corynebacterium</i>-associated hyperkeratosis and pseudotuberculosis, in immunodeficient mice. We isolated and identified a total of 165 strains of...
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MDPI AG
2022-06-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/7/328 |
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| author | Sehee Park Hijo Shin Sangwoon Kim Teakchang Lee Haejin Lee Kihoan Nam Wonkee Yoon Hyoungchin Kim Youngwon Seo Youngsuk Won Hyojung Kwon |
| author_facet | Sehee Park Hijo Shin Sangwoon Kim Teakchang Lee Haejin Lee Kihoan Nam Wonkee Yoon Hyoungchin Kim Youngwon Seo Youngsuk Won Hyojung Kwon |
| author_sort | Sehee Park |
| collection | DOAJ |
| description | The genus <i>Corynebacterium</i>, composed of Gram-positive diphtheroid rod-shaped bacteria, induces severe diseases, such as <i>Corynebacterium</i>-associated hyperkeratosis and pseudotuberculosis, in immunodeficient mice. We isolated and identified a total of 165 strains of <i>Corynebacterium</i> species from experimental mice in Korean laboratories, diagnosed using several methods. When identified based on molecular methods, namely, 16S <i>rRNA</i> and <i>rpoB</i> gene sequence analysis, the main <i>Corynebacterium</i> species isolated in Korean laboratory mice were <i>C. mastitidis</i> (44.8%, <i>n</i> = 74), <i>C. bovis</i> (25.5%, <i>n</i> = 42), <i>C. lowii</i> (21.2%, <i>n</i> = 35), and <i>C. amycolatum</i> (8.5%, <i>n</i> = 14). Diagnoses were also performed using matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) and biochemical methods. MALDI-TOF MS yielded results that were 77.9% identical to the molecular identification results, whereas biochemical methods showed only 15.5% identical to molecular identification, partly owing to difficulties in distinguishing among <i>C. mastitidis</i> strains. Collectively, our findings indicate that molecular biological methods are better suited for detecting and identifying <i>Corynebacterium</i> species candidates isolated from mice than biochemical methods. Because of limitations associated with the use of MALDI-TOF MS, more precise results will be obtained by complementing this approach with other methods when used for rapid identification testing. |
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| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T05:42:39Z |
| publishDate | 2022-06-01 |
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| series | Veterinary Sciences |
| spelling | doaj.art-e76f12e43f2242bba24dd0b09c3dcd592023-12-03T12:23:49ZengMDPI AGVeterinary Sciences2306-73812022-06-019732810.3390/vetsci9070328Distribution of <i>Corynebacterium</i> Species and Comparative Results of Diagnostic Methods for Identifying <i>Corynebacterium</i> in Experimental Mice in KoreaSehee Park0Hijo Shin1Sangwoon Kim2Teakchang Lee3Haejin Lee4Kihoan Nam5Wonkee Yoon6Hyoungchin Kim7Youngwon Seo8Youngsuk Won9Hyojung Kwon10Laboratory Animal Resource Center, Korea Research Institute of Biology and Biotechnology, 30, Yeongudanji-ro, Cheongwon-gu, Cheongju-si 28116, KoreaLaboratory Animal Resource Center, Korea Research Institute of Biology and Biotechnology, 30, Yeongudanji-ro, Cheongwon-gu, Cheongju-si 28116, KoreaLaboratory Animal Resource Center, Korea Research Institute of Biology and Biotechnology, 30, Yeongudanji-ro, Cheongwon-gu, Cheongju-si 28116, KoreaLaboratory Animal Resource Center, Korea Research Institute of Biology and Biotechnology, 30, Yeongudanji-ro, Cheongwon-gu, Cheongju-si 28116, KoreaLaboratory Animal Resource Center, Korea Research Institute of Biology and Biotechnology, 30, Yeongudanji-ro, Cheongwon-gu, Cheongju-si 28116, KoreaLaboratory Animal Resource Center, Korea Research Institute of Biology and Biotechnology, 30, Yeongudanji-ro, Cheongwon-gu, Cheongju-si 28116, KoreaLaboratory Animal Resource Center, Korea Research Institute of Biology and Biotechnology, 30, Yeongudanji-ro, Cheongwon-gu, Cheongju-si 28116, KoreaLaboratory Animal Resource Center, Korea Research Institute of Biology and Biotechnology, 30, Yeongudanji-ro, Cheongwon-gu, Cheongju-si 28116, KoreaLaboratory Animal Resource Center, Korea Research Institute of Biology and Biotechnology, 30, Yeongudanji-ro, Cheongwon-gu, Cheongju-si 28116, KoreaLaboratory Animal Resource Center, Korea Research Institute of Biology and Biotechnology, 30, Yeongudanji-ro, Cheongwon-gu, Cheongju-si 28116, KoreaDepartment of Veterinary Pathology, College of Veterinary Medicine, Chungnam National University, 99, Daehak-ro, Yuseong-gu, Daejeon 34134, KoreaThe genus <i>Corynebacterium</i>, composed of Gram-positive diphtheroid rod-shaped bacteria, induces severe diseases, such as <i>Corynebacterium</i>-associated hyperkeratosis and pseudotuberculosis, in immunodeficient mice. We isolated and identified a total of 165 strains of <i>Corynebacterium</i> species from experimental mice in Korean laboratories, diagnosed using several methods. When identified based on molecular methods, namely, 16S <i>rRNA</i> and <i>rpoB</i> gene sequence analysis, the main <i>Corynebacterium</i> species isolated in Korean laboratory mice were <i>C. mastitidis</i> (44.8%, <i>n</i> = 74), <i>C. bovis</i> (25.5%, <i>n</i> = 42), <i>C. lowii</i> (21.2%, <i>n</i> = 35), and <i>C. amycolatum</i> (8.5%, <i>n</i> = 14). Diagnoses were also performed using matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) and biochemical methods. MALDI-TOF MS yielded results that were 77.9% identical to the molecular identification results, whereas biochemical methods showed only 15.5% identical to molecular identification, partly owing to difficulties in distinguishing among <i>C. mastitidis</i> strains. Collectively, our findings indicate that molecular biological methods are better suited for detecting and identifying <i>Corynebacterium</i> species candidates isolated from mice than biochemical methods. Because of limitations associated with the use of MALDI-TOF MS, more precise results will be obtained by complementing this approach with other methods when used for rapid identification testing.https://www.mdpi.com/2306-7381/9/7/328<i>Corynebacterium</i>laboratory miceMALDI-TOF MS |
| spellingShingle | Sehee Park Hijo Shin Sangwoon Kim Teakchang Lee Haejin Lee Kihoan Nam Wonkee Yoon Hyoungchin Kim Youngwon Seo Youngsuk Won Hyojung Kwon Distribution of <i>Corynebacterium</i> Species and Comparative Results of Diagnostic Methods for Identifying <i>Corynebacterium</i> in Experimental Mice in Korea Veterinary Sciences <i>Corynebacterium</i> laboratory mice MALDI-TOF MS |
| title | Distribution of <i>Corynebacterium</i> Species and Comparative Results of Diagnostic Methods for Identifying <i>Corynebacterium</i> in Experimental Mice in Korea |
| title_full | Distribution of <i>Corynebacterium</i> Species and Comparative Results of Diagnostic Methods for Identifying <i>Corynebacterium</i> in Experimental Mice in Korea |
| title_fullStr | Distribution of <i>Corynebacterium</i> Species and Comparative Results of Diagnostic Methods for Identifying <i>Corynebacterium</i> in Experimental Mice in Korea |
| title_full_unstemmed | Distribution of <i>Corynebacterium</i> Species and Comparative Results of Diagnostic Methods for Identifying <i>Corynebacterium</i> in Experimental Mice in Korea |
| title_short | Distribution of <i>Corynebacterium</i> Species and Comparative Results of Diagnostic Methods for Identifying <i>Corynebacterium</i> in Experimental Mice in Korea |
| title_sort | distribution of i corynebacterium i species and comparative results of diagnostic methods for identifying i corynebacterium i in experimental mice in korea |
| topic | <i>Corynebacterium</i> laboratory mice MALDI-TOF MS |
| url | https://www.mdpi.com/2306-7381/9/7/328 |
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