Hyodeoxycholate-6-O-glucuronide cannot be quantitated with 3 alpha-hydroxysteroid dehydrogenase
The reaction of the 6-hydroxylated bile acid, hyodeoxycholic acid, and its 6-O-glucuronide conjugate with 3 alpha-hydroxysteroid dehydrogenase was examined. A standard end-point assay and determination of the initial rates of reaction showed only minimal activity of the enzyme toward hyodeoxycholate...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1987-11-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520386004 |
| _version_ | 1818460018930876416 |
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| author | J M Little P Zimniak A Radominska R Lester |
| author_facet | J M Little P Zimniak A Radominska R Lester |
| author_sort | J M Little |
| collection | DOAJ |
| description | The reaction of the 6-hydroxylated bile acid, hyodeoxycholic acid, and its 6-O-glucuronide conjugate with 3 alpha-hydroxysteroid dehydrogenase was examined. A standard end-point assay and determination of the initial rates of reaction showed only minimal activity of the enzyme toward hyodeoxycholate-6-glucuronide in spite of the presence of a free 3 alpha-hydroxyl group. It was established that 6-hydroxylation itself did not significantly affect the enzyme reaction. It is concluded that the 6-glucuronide either blocks or hinders enzyme access to the 3-hydroxyl group. |
| first_indexed | 2024-12-14T23:23:35Z |
| format | Article |
| id | doaj.art-e77042f790ec4a6db49b07816ba43fdf |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T23:23:35Z |
| publishDate | 1987-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-e77042f790ec4a6db49b07816ba43fdf2022-12-21T22:43:52ZengElsevierJournal of Lipid Research0022-22751987-11-01281113701372Hyodeoxycholate-6-O-glucuronide cannot be quantitated with 3 alpha-hydroxysteroid dehydrogenaseJ M Little0P Zimniak1A Radominska2R Lester3Department of Internal Medicine, University of Texas Medical School at Houston 77225.Department of Internal Medicine, University of Texas Medical School at Houston 77225.Department of Internal Medicine, University of Texas Medical School at Houston 77225.Department of Internal Medicine, University of Texas Medical School at Houston 77225.The reaction of the 6-hydroxylated bile acid, hyodeoxycholic acid, and its 6-O-glucuronide conjugate with 3 alpha-hydroxysteroid dehydrogenase was examined. A standard end-point assay and determination of the initial rates of reaction showed only minimal activity of the enzyme toward hyodeoxycholate-6-glucuronide in spite of the presence of a free 3 alpha-hydroxyl group. It was established that 6-hydroxylation itself did not significantly affect the enzyme reaction. It is concluded that the 6-glucuronide either blocks or hinders enzyme access to the 3-hydroxyl group.http://www.sciencedirect.com/science/article/pii/S0022227520386004 |
| spellingShingle | J M Little P Zimniak A Radominska R Lester Hyodeoxycholate-6-O-glucuronide cannot be quantitated with 3 alpha-hydroxysteroid dehydrogenase Journal of Lipid Research |
| title | Hyodeoxycholate-6-O-glucuronide cannot be quantitated with 3 alpha-hydroxysteroid dehydrogenase |
| title_full | Hyodeoxycholate-6-O-glucuronide cannot be quantitated with 3 alpha-hydroxysteroid dehydrogenase |
| title_fullStr | Hyodeoxycholate-6-O-glucuronide cannot be quantitated with 3 alpha-hydroxysteroid dehydrogenase |
| title_full_unstemmed | Hyodeoxycholate-6-O-glucuronide cannot be quantitated with 3 alpha-hydroxysteroid dehydrogenase |
| title_short | Hyodeoxycholate-6-O-glucuronide cannot be quantitated with 3 alpha-hydroxysteroid dehydrogenase |
| title_sort | hyodeoxycholate 6 o glucuronide cannot be quantitated with 3 alpha hydroxysteroid dehydrogenase |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520386004 |
| work_keys_str_mv | AT jmlittle hyodeoxycholate6oglucuronidecannotbequantitatedwith3alphahydroxysteroiddehydrogenase AT pzimniak hyodeoxycholate6oglucuronidecannotbequantitatedwith3alphahydroxysteroiddehydrogenase AT aradominska hyodeoxycholate6oglucuronidecannotbequantitatedwith3alphahydroxysteroiddehydrogenase AT rlester hyodeoxycholate6oglucuronidecannotbequantitatedwith3alphahydroxysteroiddehydrogenase |