Genetic Circuit Design in Rhizobacteria
Genetically engineered plants hold enormous promise for tackling global food security and agricultural sustainability challenges. However, construction of plant-based genetic circuitry is constrained by a lack of well-characterized genetic parts and circuit design rules. In contrast, advances in bac...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
American Association for the Advancement of Science (AAAS)
2022-01-01
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| Series: | BioDesign Research |
| Online Access: | http://dx.doi.org/10.34133/2022/9858049 |
| _version_ | 1797281508765990912 |
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| author | Christopher M. Dundas José R. Dinneny |
| author_facet | Christopher M. Dundas José R. Dinneny |
| author_sort | Christopher M. Dundas |
| collection | DOAJ |
| description | Genetically engineered plants hold enormous promise for tackling global food security and agricultural sustainability challenges. However, construction of plant-based genetic circuitry is constrained by a lack of well-characterized genetic parts and circuit design rules. In contrast, advances in bacterial synthetic biology have yielded a wealth of sensors, actuators, and other tools that can be used to build bacterial circuitry. As root-colonizing bacteria (rhizobacteria) exert substantial influence over plant health and growth, genetic circuit design in these microorganisms can be used to indirectly engineer plants and accelerate the design-build-test-learn cycle. Here, we outline genetic parts and best practices for designing rhizobacterial circuits, with an emphasis on sensors, actuators, and chassis species that can be used to monitor/control rhizosphere and plant processes. |
| first_indexed | 2024-03-07T16:58:32Z |
| format | Article |
| id | doaj.art-e77159dbadc8477db0471939f1adbf6f |
| institution | Directory Open Access Journal |
| issn | 2693-1257 |
| language | English |
| last_indexed | 2024-03-07T16:58:32Z |
| publishDate | 2022-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | BioDesign Research |
| spelling | doaj.art-e77159dbadc8477db0471939f1adbf6f2024-03-03T03:31:34ZengAmerican Association for the Advancement of Science (AAAS)BioDesign Research2693-12572022-01-01202210.34133/2022/9858049Genetic Circuit Design in RhizobacteriaChristopher M. Dundas0José R. Dinneny1Department of Biology, Stanford University, Stanford, CA 94305, USADepartment of Biology, Stanford University, Stanford, CA 94305, USAGenetically engineered plants hold enormous promise for tackling global food security and agricultural sustainability challenges. However, construction of plant-based genetic circuitry is constrained by a lack of well-characterized genetic parts and circuit design rules. In contrast, advances in bacterial synthetic biology have yielded a wealth of sensors, actuators, and other tools that can be used to build bacterial circuitry. As root-colonizing bacteria (rhizobacteria) exert substantial influence over plant health and growth, genetic circuit design in these microorganisms can be used to indirectly engineer plants and accelerate the design-build-test-learn cycle. Here, we outline genetic parts and best practices for designing rhizobacterial circuits, with an emphasis on sensors, actuators, and chassis species that can be used to monitor/control rhizosphere and plant processes.http://dx.doi.org/10.34133/2022/9858049 |
| spellingShingle | Christopher M. Dundas José R. Dinneny Genetic Circuit Design in Rhizobacteria BioDesign Research |
| title | Genetic Circuit Design in Rhizobacteria |
| title_full | Genetic Circuit Design in Rhizobacteria |
| title_fullStr | Genetic Circuit Design in Rhizobacteria |
| title_full_unstemmed | Genetic Circuit Design in Rhizobacteria |
| title_short | Genetic Circuit Design in Rhizobacteria |
| title_sort | genetic circuit design in rhizobacteria |
| url | http://dx.doi.org/10.34133/2022/9858049 |
| work_keys_str_mv | AT christophermdundas geneticcircuitdesigninrhizobacteria AT joserdinneny geneticcircuitdesigninrhizobacteria |