Alterations in urinary microbiota composition in urolithiasis patients: insights from 16S rRNA gene sequencing
ObjectivesTo investigate the urinary microbiota composition in urolithiasis patients compared to healthy controls and to identify potential microbial markers and their association with clinical parameters.MethodsA total of 66 samples, comprising 45 from urolithiasis patients and 21 from healthy cont...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1266446/full |
| _version_ | 1797644378715455488 |
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| author | Haoran Liu Haoran Liu Qingqing Hu Qingqing Hu Qunsheng Yan Qunsheng Yan Zongyao Hao Zongyao Hao Chaozhao Liang Chaozhao Liang |
| author_facet | Haoran Liu Haoran Liu Qingqing Hu Qingqing Hu Qunsheng Yan Qunsheng Yan Zongyao Hao Zongyao Hao Chaozhao Liang Chaozhao Liang |
| author_sort | Haoran Liu |
| collection | DOAJ |
| description | ObjectivesTo investigate the urinary microbiota composition in urolithiasis patients compared to healthy controls and to identify potential microbial markers and their association with clinical parameters.MethodsA total of 66 samples, comprising 45 from urolithiasis patients and 21 from healthy controls, were analyzed. 16S rRNA gene sequencing was employed to determine the microbiota composition. Various statistical and bioinformatics tools, including ANOVA, PCoA, and LEfSe, were utilized to analyze the sequencing data and identify significant differences in microbial abundance.ResultsNo significant demographic differences were observed between the two groups. Post-quality control, clean tags ranged from 60,979 to 68,736. Significant differences in α-diversity were observed between the two groups. β-diversity analysis revealed distinct clustering of the urinary microbiota in urolithiasis patients and controls. Notably, Ruminococcaceae was predominant in urolithiasis samples, while Proteobacteria was more prevalent in healthy samples. Lactobacillus was significantly overrepresented in samples from healthy females.ConclusionThe urinary microbiota composition in urolithiasis patients is distinct from that of healthy controls. Specific microbial taxa, such as Ruminococcaceae and Proteobacteria, could serve as potential biomarkers for urolithiasis. The findings pave the way for further exploration of the role of microbiota in urolithiasis and the development of microbiome-based therapeutic strategies. |
| first_indexed | 2024-03-11T14:29:40Z |
| format | Article |
| id | doaj.art-e777aa055f0e4c8fba852fa9a07b6148 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-11T14:29:40Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-e777aa055f0e4c8fba852fa9a07b61482023-10-31T11:45:32ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-10-011310.3389/fcimb.2023.12664461266446Alterations in urinary microbiota composition in urolithiasis patients: insights from 16S rRNA gene sequencingHaoran Liu0Haoran Liu1Qingqing Hu2Qingqing Hu3Qunsheng Yan4Qunsheng Yan5Zongyao Hao6Zongyao Hao7Chaozhao Liang8Chaozhao Liang9Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaInstitute of Urology & Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, ChinaDepartment of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaInstitute of Urology & Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, ChinaDepartment of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaInstitute of Urology & Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, ChinaDepartment of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaInstitute of Urology & Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, ChinaDepartment of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, ChinaInstitute of Urology & Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, ChinaObjectivesTo investigate the urinary microbiota composition in urolithiasis patients compared to healthy controls and to identify potential microbial markers and their association with clinical parameters.MethodsA total of 66 samples, comprising 45 from urolithiasis patients and 21 from healthy controls, were analyzed. 16S rRNA gene sequencing was employed to determine the microbiota composition. Various statistical and bioinformatics tools, including ANOVA, PCoA, and LEfSe, were utilized to analyze the sequencing data and identify significant differences in microbial abundance.ResultsNo significant demographic differences were observed between the two groups. Post-quality control, clean tags ranged from 60,979 to 68,736. Significant differences in α-diversity were observed between the two groups. β-diversity analysis revealed distinct clustering of the urinary microbiota in urolithiasis patients and controls. Notably, Ruminococcaceae was predominant in urolithiasis samples, while Proteobacteria was more prevalent in healthy samples. Lactobacillus was significantly overrepresented in samples from healthy females.ConclusionThe urinary microbiota composition in urolithiasis patients is distinct from that of healthy controls. Specific microbial taxa, such as Ruminococcaceae and Proteobacteria, could serve as potential biomarkers for urolithiasis. The findings pave the way for further exploration of the role of microbiota in urolithiasis and the development of microbiome-based therapeutic strategies.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1266446/fullurinary microbiotaurolithiasis16S rRNA gene sequencingmicrobial diversityclinical correlations |
| spellingShingle | Haoran Liu Haoran Liu Qingqing Hu Qingqing Hu Qunsheng Yan Qunsheng Yan Zongyao Hao Zongyao Hao Chaozhao Liang Chaozhao Liang Alterations in urinary microbiota composition in urolithiasis patients: insights from 16S rRNA gene sequencing Frontiers in Cellular and Infection Microbiology urinary microbiota urolithiasis 16S rRNA gene sequencing microbial diversity clinical correlations |
| title | Alterations in urinary microbiota composition in urolithiasis patients: insights from 16S rRNA gene sequencing |
| title_full | Alterations in urinary microbiota composition in urolithiasis patients: insights from 16S rRNA gene sequencing |
| title_fullStr | Alterations in urinary microbiota composition in urolithiasis patients: insights from 16S rRNA gene sequencing |
| title_full_unstemmed | Alterations in urinary microbiota composition in urolithiasis patients: insights from 16S rRNA gene sequencing |
| title_short | Alterations in urinary microbiota composition in urolithiasis patients: insights from 16S rRNA gene sequencing |
| title_sort | alterations in urinary microbiota composition in urolithiasis patients insights from 16s rrna gene sequencing |
| topic | urinary microbiota urolithiasis 16S rRNA gene sequencing microbial diversity clinical correlations |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1266446/full |
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