Extended field-of-view light-sheet microscopy
Light-sheet fluorescence microscopy enables rapid 3D imaging of biological samples. Unlike confocal and two-photon microscopes, a light-sheet microscope illuminates the focal plane with an objective orthogonal to the detection axis and images it in a single snapshot. Its combination of high contrast...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2022-01-01
|
| Series: | EPJ Web of Conferences |
| Online Access: | https://www.epj-conferences.org/articles/epjconf/pdf/2022/10/epjconf_eosam2022_02009.pdf |
| _version_ | 1797990546443075584 |
|---|---|
| author | Vettenburg Tom |
| author_facet | Vettenburg Tom |
| author_sort | Vettenburg Tom |
| collection | DOAJ |
| description | Light-sheet fluorescence microscopy enables rapid 3D imaging of biological samples. Unlike confocal and two-photon microscopes, a light-sheet microscope illuminates the focal plane with an objective orthogonal to the detection axis and images it in a single snapshot. Its combination of high contrast and minimal sample exposure make it ideal to image thick samples with sub-cellular resolution. To uniformly illuminate a wide field-of-view without compromising axial resolution, propagation-invariant light-fields such as Bessel and Airy beams have been put forward. These beams do however irradiate the sample with a relatively broad transversal structure. The fluorescence excited by the side lobes of Bessel beams can be blocked physically during recording; though at the cost of increased sample exposure. In contrast, the Airy beam has a fine transversal structure that is both curved and asymmetric. Its fine structure captures all the high-frequency components that enable high axial resolution without the need to discard useful fluorescence. This advantage does not carry over naturally to two-photon excitation where the fine transversal structure is suppressed. We demonstrate a symmetric and planar Airy light-sheet that can be used with two-photon excitation and that does not rely on deconvolution. |
| first_indexed | 2024-04-11T08:38:14Z |
| format | Article |
| id | doaj.art-e77a3ac52e5b4b83afc5461e8bd1898e |
| institution | Directory Open Access Journal |
| issn | 2100-014X |
| language | English |
| last_indexed | 2024-04-11T08:38:14Z |
| publishDate | 2022-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | EPJ Web of Conferences |
| spelling | doaj.art-e77a3ac52e5b4b83afc5461e8bd1898e2022-12-22T04:34:17ZengEDP SciencesEPJ Web of Conferences2100-014X2022-01-012660200910.1051/epjconf/202226602009epjconf_eosam2022_02009Extended field-of-view light-sheet microscopyVettenburg Tom0School of Science and Engineering, University of DundeeLight-sheet fluorescence microscopy enables rapid 3D imaging of biological samples. Unlike confocal and two-photon microscopes, a light-sheet microscope illuminates the focal plane with an objective orthogonal to the detection axis and images it in a single snapshot. Its combination of high contrast and minimal sample exposure make it ideal to image thick samples with sub-cellular resolution. To uniformly illuminate a wide field-of-view without compromising axial resolution, propagation-invariant light-fields such as Bessel and Airy beams have been put forward. These beams do however irradiate the sample with a relatively broad transversal structure. The fluorescence excited by the side lobes of Bessel beams can be blocked physically during recording; though at the cost of increased sample exposure. In contrast, the Airy beam has a fine transversal structure that is both curved and asymmetric. Its fine structure captures all the high-frequency components that enable high axial resolution without the need to discard useful fluorescence. This advantage does not carry over naturally to two-photon excitation where the fine transversal structure is suppressed. We demonstrate a symmetric and planar Airy light-sheet that can be used with two-photon excitation and that does not rely on deconvolution.https://www.epj-conferences.org/articles/epjconf/pdf/2022/10/epjconf_eosam2022_02009.pdf |
| spellingShingle | Vettenburg Tom Extended field-of-view light-sheet microscopy EPJ Web of Conferences |
| title | Extended field-of-view light-sheet microscopy |
| title_full | Extended field-of-view light-sheet microscopy |
| title_fullStr | Extended field-of-view light-sheet microscopy |
| title_full_unstemmed | Extended field-of-view light-sheet microscopy |
| title_short | Extended field-of-view light-sheet microscopy |
| title_sort | extended field of view light sheet microscopy |
| url | https://www.epj-conferences.org/articles/epjconf/pdf/2022/10/epjconf_eosam2022_02009.pdf |
| work_keys_str_mv | AT vettenburgtom extendedfieldofviewlightsheetmicroscopy |