Europium(III) as luminescence probe for interactions of a sulfate-reducing microorganism with potentially toxic metals
Microorganisms show a high affinity for trivalent actinides and lanthanides, which play an important role in the safe disposal of high-level radioactive waste as well as in the mining of various rare earth elements. The interaction of the lanthanide Eu(III) with the sulfate-reducing microorganism De...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-10-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323009788 |
| _version_ | 1797676653952892928 |
|---|---|
| author | Stephan Hilpmann Henry Moll Björn Drobot Manja Vogel René Hübner Thorsten Stumpf Andrea Cherkouk |
| author_facet | Stephan Hilpmann Henry Moll Björn Drobot Manja Vogel René Hübner Thorsten Stumpf Andrea Cherkouk |
| author_sort | Stephan Hilpmann |
| collection | DOAJ |
| description | Microorganisms show a high affinity for trivalent actinides and lanthanides, which play an important role in the safe disposal of high-level radioactive waste as well as in the mining of various rare earth elements. The interaction of the lanthanide Eu(III) with the sulfate-reducing microorganism Desulfosporosinus hippei DSM 8344T, a representative of the genus Desulfosporosinus that naturally occurs in clay rock and bentonite, was investigated. Eu(III) is often used as a non-radioactive analogue for the trivalent actinides Pu(III), Am(III), and Cm(III), which contribute to a major part of the radiotoxicity of the nuclear waste. D. hippei DSM 8344T showed a weak interaction with Eu(III), most likely due to a complexation with lactate in artificial Opalinus Clay pore water. Hence, a low removal of the lanthanide from the supernatant was observed. Scanning transmission electron microscopy coupled with energy-dispersive X-ray spectroscopy revealed a bioprecipitation of Eu(III) with phosphates potentially excreted from the cells. This demonstrates that the ongoing interaction mechanisms are more complex than a simple biosorption process. The bioprecipitation was also verified by luminescence spectroscopy, which showed that the formation of the Eu(III) phosphate compounds starts almost immediately after the addition of the cells. Moreover, chemical microscopy provided information on the local distribution of the different Eu(III) species in the formed cell aggregates. These results provide first insights into the interaction mechanisms of Eu(III) with sulfate-reducing bacteria and contribute to a comprehensive safety concept for a high-level radioactive waste repository, as well as to a better understanding of the fate of heavy metals (especially rare earth elements) in the environment. |
| first_indexed | 2024-03-11T22:32:23Z |
| format | Article |
| id | doaj.art-e7802af6c3af4fec8a03d34be2f7a224 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-11T22:32:23Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-e7802af6c3af4fec8a03d34be2f7a2242023-09-23T05:10:10ZengElsevierEcotoxicology and Environmental Safety0147-65132023-10-01264115474Europium(III) as luminescence probe for interactions of a sulfate-reducing microorganism with potentially toxic metalsStephan Hilpmann0Henry Moll1Björn Drobot2Manja Vogel3René Hübner4Thorsten Stumpf5Andrea Cherkouk6Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstraße 400, 01328 Dresden, GermanyHelmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstraße 400, 01328 Dresden, GermanyHelmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstraße 400, 01328 Dresden, GermanyVKTA – Strahlenschutz, Analytik & Entsorgung Rossendorf e. V., Dresden, GermanyHelmholtz-Zentrum Dresden-Rossendorf, Institute of Ion Beam Physics and Materials Research, Dresden, GermanyHelmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstraße 400, 01328 Dresden, GermanyHelmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstraße 400, 01328 Dresden, Germany; Corresponding author.Microorganisms show a high affinity for trivalent actinides and lanthanides, which play an important role in the safe disposal of high-level radioactive waste as well as in the mining of various rare earth elements. The interaction of the lanthanide Eu(III) with the sulfate-reducing microorganism Desulfosporosinus hippei DSM 8344T, a representative of the genus Desulfosporosinus that naturally occurs in clay rock and bentonite, was investigated. Eu(III) is often used as a non-radioactive analogue for the trivalent actinides Pu(III), Am(III), and Cm(III), which contribute to a major part of the radiotoxicity of the nuclear waste. D. hippei DSM 8344T showed a weak interaction with Eu(III), most likely due to a complexation with lactate in artificial Opalinus Clay pore water. Hence, a low removal of the lanthanide from the supernatant was observed. Scanning transmission electron microscopy coupled with energy-dispersive X-ray spectroscopy revealed a bioprecipitation of Eu(III) with phosphates potentially excreted from the cells. This demonstrates that the ongoing interaction mechanisms are more complex than a simple biosorption process. The bioprecipitation was also verified by luminescence spectroscopy, which showed that the formation of the Eu(III) phosphate compounds starts almost immediately after the addition of the cells. Moreover, chemical microscopy provided information on the local distribution of the different Eu(III) species in the formed cell aggregates. These results provide first insights into the interaction mechanisms of Eu(III) with sulfate-reducing bacteria and contribute to a comprehensive safety concept for a high-level radioactive waste repository, as well as to a better understanding of the fate of heavy metals (especially rare earth elements) in the environment.http://www.sciencedirect.com/science/article/pii/S0147651323009788Europium(III) luminescenceSulfate-reducing bacteriaEuropium(III) bioprecipitationOpalinus clay pore water |
| spellingShingle | Stephan Hilpmann Henry Moll Björn Drobot Manja Vogel René Hübner Thorsten Stumpf Andrea Cherkouk Europium(III) as luminescence probe for interactions of a sulfate-reducing microorganism with potentially toxic metals Ecotoxicology and Environmental Safety Europium(III) luminescence Sulfate-reducing bacteria Europium(III) bioprecipitation Opalinus clay pore water |
| title | Europium(III) as luminescence probe for interactions of a sulfate-reducing microorganism with potentially toxic metals |
| title_full | Europium(III) as luminescence probe for interactions of a sulfate-reducing microorganism with potentially toxic metals |
| title_fullStr | Europium(III) as luminescence probe for interactions of a sulfate-reducing microorganism with potentially toxic metals |
| title_full_unstemmed | Europium(III) as luminescence probe for interactions of a sulfate-reducing microorganism with potentially toxic metals |
| title_short | Europium(III) as luminescence probe for interactions of a sulfate-reducing microorganism with potentially toxic metals |
| title_sort | europium iii as luminescence probe for interactions of a sulfate reducing microorganism with potentially toxic metals |
| topic | Europium(III) luminescence Sulfate-reducing bacteria Europium(III) bioprecipitation Opalinus clay pore water |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323009788 |
| work_keys_str_mv | AT stephanhilpmann europiumiiiasluminescenceprobeforinteractionsofasulfatereducingmicroorganismwithpotentiallytoxicmetals AT henrymoll europiumiiiasluminescenceprobeforinteractionsofasulfatereducingmicroorganismwithpotentiallytoxicmetals AT bjorndrobot europiumiiiasluminescenceprobeforinteractionsofasulfatereducingmicroorganismwithpotentiallytoxicmetals AT manjavogel europiumiiiasluminescenceprobeforinteractionsofasulfatereducingmicroorganismwithpotentiallytoxicmetals AT renehubner europiumiiiasluminescenceprobeforinteractionsofasulfatereducingmicroorganismwithpotentiallytoxicmetals AT thorstenstumpf europiumiiiasluminescenceprobeforinteractionsofasulfatereducingmicroorganismwithpotentiallytoxicmetals AT andreacherkouk europiumiiiasluminescenceprobeforinteractionsofasulfatereducingmicroorganismwithpotentiallytoxicmetals |