Molecular detection of Theileria spp. in native sheep and estimation of hemato-biochemical parameters from Sulaimani province/Iraq
Theileriosis, the hemoprotozoan infection, is an endemic condition in tropical and subtropical areas. In this study, conventional PCR analysis was applied to detect the natural infection of native sheep with theileriosis and estimate its effect on hemato-biochemical parameters. The study was carried...
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.1059599/full |
| _version_ | 1811184360046460928 |
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| author | Shadan Hassan Abdullah Hiewa Othman Dyary Nahla Mohammed Saeed |
| author_facet | Shadan Hassan Abdullah Hiewa Othman Dyary Nahla Mohammed Saeed |
| author_sort | Shadan Hassan Abdullah |
| collection | DOAJ |
| description | Theileriosis, the hemoprotozoan infection, is an endemic condition in tropical and subtropical areas. In this study, conventional PCR analysis was applied to detect the natural infection of native sheep with theileriosis and estimate its effect on hemato-biochemical parameters. The study was carried out in five regions of Sulaimani province, northern Iraq. From May to October 2021, a total of 360 blood samples were collected randomly from the jugular vein of sheep belonging to 23 flocks with a history of tick infestations. After PCR for theileriosis, the hematobiochemical parameters were evaluated by an automatic analyzer using commercial kits. The PCR results represented that 71.7% of the examined sheep were infected with Theileria parasites. The highest prevalence rate (74.6%) was reported in Said Sadiq, and the lowest prevalence (69.5%) was from Bazian. The infection rates in Mawat, Qaradagh, and Sharazoor were 73.1, 70.3, and 71.8%, respectively. The hemogram data revealed a significant decrease in erythrocyte count, hemoglobin concentration, and hematocrit values. Erythrocyte indices also showed significant increases in MC, MCH, and MCHC levels, but no significant differences were detected between the counting of leukocytes, lymphocytes, and granulocytes. Biochemical analysis revealed a significant decrease in total protein, albumin, and creatinine levels with a significant increase in urea and AST levels in infected sheep with theileriosis. Alteration in hemato-biochemical parameters from infected animals can outline the impact of theileriosis on body metabolism and blood factors in naturally infected sheep. |
| first_indexed | 2024-04-11T13:12:01Z |
| format | Article |
| id | doaj.art-e780de61456545388f5ca311341f6747 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-11T13:12:01Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-e780de61456545388f5ca311341f67472022-12-22T04:22:34ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-12-01910.3389/fvets.2022.10595991059599Molecular detection of Theileria spp. in native sheep and estimation of hemato-biochemical parameters from Sulaimani province/IraqShadan Hassan Abdullah0Hiewa Othman Dyary1Nahla Mohammed Saeed2Department of Microbiology, College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, IraqDepartment of Basic Sciences, College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, IraqDepartment of Microbiology, College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, IraqTheileriosis, the hemoprotozoan infection, is an endemic condition in tropical and subtropical areas. In this study, conventional PCR analysis was applied to detect the natural infection of native sheep with theileriosis and estimate its effect on hemato-biochemical parameters. The study was carried out in five regions of Sulaimani province, northern Iraq. From May to October 2021, a total of 360 blood samples were collected randomly from the jugular vein of sheep belonging to 23 flocks with a history of tick infestations. After PCR for theileriosis, the hematobiochemical parameters were evaluated by an automatic analyzer using commercial kits. The PCR results represented that 71.7% of the examined sheep were infected with Theileria parasites. The highest prevalence rate (74.6%) was reported in Said Sadiq, and the lowest prevalence (69.5%) was from Bazian. The infection rates in Mawat, Qaradagh, and Sharazoor were 73.1, 70.3, and 71.8%, respectively. The hemogram data revealed a significant decrease in erythrocyte count, hemoglobin concentration, and hematocrit values. Erythrocyte indices also showed significant increases in MC, MCH, and MCHC levels, but no significant differences were detected between the counting of leukocytes, lymphocytes, and granulocytes. Biochemical analysis revealed a significant decrease in total protein, albumin, and creatinine levels with a significant increase in urea and AST levels in infected sheep with theileriosis. Alteration in hemato-biochemical parameters from infected animals can outline the impact of theileriosis on body metabolism and blood factors in naturally infected sheep.https://www.frontiersin.org/articles/10.3389/fvets.2022.1059599/fullTheileriasheepbloodPCRSulaimani |
| spellingShingle | Shadan Hassan Abdullah Hiewa Othman Dyary Nahla Mohammed Saeed Molecular detection of Theileria spp. in native sheep and estimation of hemato-biochemical parameters from Sulaimani province/Iraq Frontiers in Veterinary Science Theileria sheep blood PCR Sulaimani |
| title | Molecular detection of Theileria spp. in native sheep and estimation of hemato-biochemical parameters from Sulaimani province/Iraq |
| title_full | Molecular detection of Theileria spp. in native sheep and estimation of hemato-biochemical parameters from Sulaimani province/Iraq |
| title_fullStr | Molecular detection of Theileria spp. in native sheep and estimation of hemato-biochemical parameters from Sulaimani province/Iraq |
| title_full_unstemmed | Molecular detection of Theileria spp. in native sheep and estimation of hemato-biochemical parameters from Sulaimani province/Iraq |
| title_short | Molecular detection of Theileria spp. in native sheep and estimation of hemato-biochemical parameters from Sulaimani province/Iraq |
| title_sort | molecular detection of theileria spp in native sheep and estimation of hemato biochemical parameters from sulaimani province iraq |
| topic | Theileria sheep blood PCR Sulaimani |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.1059599/full |
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