Extracellular Vesicles from Uterine Aspirates Represent a Promising Source for Screening Markers of Gynecologic Cancers
Extracellular vesicles (EVs), including exosomes, are key factors of intercellular communication, performing both local and distant transfers of bioactive molecules. The increasingly obvious role of EVs in carcinogenesis, similarity of molecular signatures with parental cells, precise selection and...
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MDPI AG
2022-03-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/7/1064 |
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| author | Gleb O. Skryabin Andrey V. Komelkov Kirill I. Zhordania Dmitry V. Bagrov Svetlana V. Vinokurova Sergey A. Galetsky Nadezhda V. Elkina Darya A. Denisova Adel D. Enikeev Elena M. Tchevkina |
| author_facet | Gleb O. Skryabin Andrey V. Komelkov Kirill I. Zhordania Dmitry V. Bagrov Svetlana V. Vinokurova Sergey A. Galetsky Nadezhda V. Elkina Darya A. Denisova Adel D. Enikeev Elena M. Tchevkina |
| author_sort | Gleb O. Skryabin |
| collection | DOAJ |
| description | Extracellular vesicles (EVs), including exosomes, are key factors of intercellular communication, performing both local and distant transfers of bioactive molecules. The increasingly obvious role of EVs in carcinogenesis, similarity of molecular signatures with parental cells, precise selection and high stability of cargo molecules make exosomes a promising source of liquid biopsy markers for cancer diagnosis. The uterine cavity fluid, unlike blood, urine and other body fluids commonly used to study EVs, is of local origin and therefore enriched in EVs secreted by cells of the female reproductive tract. Here, we show that EVs, including those corresponding to exosomes, could be isolated from individual samples of uterine aspirates (UA) obtained from epithelial ovarian cancer (EOC) patients and healthy donors using the ultracentrifugation technique. First, the conducted profiling of small RNAs (small RNA-seq) from UA-derived EVs demonstrated the presence of non-coding RNA molecules belonging to various classes. The analysis of the miRNA content in EVs from UA performed on a pilot sample revealed significant differences in the expression levels of a number of miRNAs in EVs obtained from EOC patients compared to healthy individuals. The results open up prospects for using UA-derived EVs as a source of markers for the diagnostics of gynecological cancers, including EOC. |
| first_indexed | 2024-03-09T12:02:11Z |
| format | Article |
| id | doaj.art-e78175828b1e46c3adf856fc92425ada |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T12:02:11Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-e78175828b1e46c3adf856fc92425ada2023-11-30T23:02:59ZengMDPI AGCells2073-44092022-03-01117106410.3390/cells11071064Extracellular Vesicles from Uterine Aspirates Represent a Promising Source for Screening Markers of Gynecologic CancersGleb O. Skryabin0Andrey V. Komelkov1Kirill I. Zhordania2Dmitry V. Bagrov3Svetlana V. Vinokurova4Sergey A. Galetsky5Nadezhda V. Elkina6Darya A. Denisova7Adel D. Enikeev8Elena M. Tchevkina9Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Kashirskoye sh. 24, 115478 Moscow, RussiaInstitute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Kashirskoye sh. 24, 115478 Moscow, RussiaInstitute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Kashirskoye sh. 24, 115478 Moscow, RussiaDepartment of Bioengineering, Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory, 1/12, 111234 Moscow, RussiaInstitute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Kashirskoye sh. 24, 115478 Moscow, RussiaInstitute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Kashirskoye sh. 24, 115478 Moscow, RussiaInstitute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Kashirskoye sh. 24, 115478 Moscow, RussiaInstitute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Kashirskoye sh. 24, 115478 Moscow, RussiaInstitute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Kashirskoye sh. 24, 115478 Moscow, RussiaInstitute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology, Kashirskoye sh. 24, 115478 Moscow, RussiaExtracellular vesicles (EVs), including exosomes, are key factors of intercellular communication, performing both local and distant transfers of bioactive molecules. The increasingly obvious role of EVs in carcinogenesis, similarity of molecular signatures with parental cells, precise selection and high stability of cargo molecules make exosomes a promising source of liquid biopsy markers for cancer diagnosis. The uterine cavity fluid, unlike blood, urine and other body fluids commonly used to study EVs, is of local origin and therefore enriched in EVs secreted by cells of the female reproductive tract. Here, we show that EVs, including those corresponding to exosomes, could be isolated from individual samples of uterine aspirates (UA) obtained from epithelial ovarian cancer (EOC) patients and healthy donors using the ultracentrifugation technique. First, the conducted profiling of small RNAs (small RNA-seq) from UA-derived EVs demonstrated the presence of non-coding RNA molecules belonging to various classes. The analysis of the miRNA content in EVs from UA performed on a pilot sample revealed significant differences in the expression levels of a number of miRNAs in EVs obtained from EOC patients compared to healthy individuals. The results open up prospects for using UA-derived EVs as a source of markers for the diagnostics of gynecological cancers, including EOC.https://www.mdpi.com/2073-4409/11/7/1064miRNAovarian canceruterine aspiratesextracellular vesiclesexosomessmall RNA sequencing |
| spellingShingle | Gleb O. Skryabin Andrey V. Komelkov Kirill I. Zhordania Dmitry V. Bagrov Svetlana V. Vinokurova Sergey A. Galetsky Nadezhda V. Elkina Darya A. Denisova Adel D. Enikeev Elena M. Tchevkina Extracellular Vesicles from Uterine Aspirates Represent a Promising Source for Screening Markers of Gynecologic Cancers Cells miRNA ovarian cancer uterine aspirates extracellular vesicles exosomes small RNA sequencing |
| title | Extracellular Vesicles from Uterine Aspirates Represent a Promising Source for Screening Markers of Gynecologic Cancers |
| title_full | Extracellular Vesicles from Uterine Aspirates Represent a Promising Source for Screening Markers of Gynecologic Cancers |
| title_fullStr | Extracellular Vesicles from Uterine Aspirates Represent a Promising Source for Screening Markers of Gynecologic Cancers |
| title_full_unstemmed | Extracellular Vesicles from Uterine Aspirates Represent a Promising Source for Screening Markers of Gynecologic Cancers |
| title_short | Extracellular Vesicles from Uterine Aspirates Represent a Promising Source for Screening Markers of Gynecologic Cancers |
| title_sort | extracellular vesicles from uterine aspirates represent a promising source for screening markers of gynecologic cancers |
| topic | miRNA ovarian cancer uterine aspirates extracellular vesicles exosomes small RNA sequencing |
| url | https://www.mdpi.com/2073-4409/11/7/1064 |
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