P10.08 PULMONARY ARTERY CALCIFICATION IN RACEHORSES
Vascular calcification (VC) has been sporadically reported in horses, but little is known regarding cause, pathogenesis and clinical significance. We hypothesized that in horses, structural and molecular changes may occur during VC that are comparable to human and mouse models. We surveyed Thorough...
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| Format: | Article |
| Language: | English |
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BMC
2009-12-01
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| Series: | Artery Research |
| Online Access: | https://www.atlantis-press.com/article/125927375/view |
| _version_ | 1811301412709072896 |
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| author | L.G. Arroyo M.A. Hayes L. Viel |
| author_facet | L.G. Arroyo M.A. Hayes L. Viel |
| author_sort | L.G. Arroyo |
| collection | DOAJ |
| description | Vascular calcification (VC) has been sporadically reported in horses, but little is known regarding cause, pathogenesis and clinical significance.
We hypothesized that in horses, structural and molecular changes may occur during VC that are comparable to human and mouse models. We surveyed Thoroughbred and Standardbred racehorses (n=101) for the prevalence, distribution and severity of VC. Histopathological, ultrastructural imaging and energy dispersive X-ray elemental analyses were used to examine the lesions. Immunohistochemistry for cell markers (smooth muscle α-actin, SM22α and Sox9) was performed in selected samples from control (n=10), mildly (n=10), and severely (n=10) calcified arteries.
Results showed that calcification of the tunica media of mainly the pulmonary artery branches, was present in 82% of horses, and both breeds and genders were similarly affected. Lesions appeared as white-to-yellowish, hard, gritty plaques of variable size. Microscopically, elastic fibers were thin, fragmented and calcified, and surrounded by dense collagen matrix, as described for Mönckeberg sclerosis. Elemental analysis of the calcified areas was consistent with hydroxyapatite mineral.
No immunoreactivity for the smooth muscle cell markers, smooth muscle α-actin and SM22α was observed in cells found at the calcification site. Many of these cells had a chondrocytic phenotype appearance and showed immunoreactivity for Sox9, a chondrocyte marker.
Arterial calcification in horses share histopathological features with arterial medial calcification in humans and may result in similar physiological abnormalities such as vascular stiffness. The occurrence of VC in young racing horses indicates the need to investigate its pathogenesis and potential clinical implications. |
| first_indexed | 2024-04-13T07:08:21Z |
| format | Article |
| id | doaj.art-e7818dc7bc0443d8a9d02255181eea16 |
| institution | Directory Open Access Journal |
| issn | 1876-4401 |
| language | English |
| last_indexed | 2024-04-13T07:08:21Z |
| publishDate | 2009-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Artery Research |
| spelling | doaj.art-e7818dc7bc0443d8a9d02255181eea162022-12-22T02:56:56ZengBMCArtery Research1876-44012009-12-013410.1016/j.artres.2009.10.139P10.08 PULMONARY ARTERY CALCIFICATION IN RACEHORSESL.G. ArroyoM.A. HayesL. VielVascular calcification (VC) has been sporadically reported in horses, but little is known regarding cause, pathogenesis and clinical significance. We hypothesized that in horses, structural and molecular changes may occur during VC that are comparable to human and mouse models. We surveyed Thoroughbred and Standardbred racehorses (n=101) for the prevalence, distribution and severity of VC. Histopathological, ultrastructural imaging and energy dispersive X-ray elemental analyses were used to examine the lesions. Immunohistochemistry for cell markers (smooth muscle α-actin, SM22α and Sox9) was performed in selected samples from control (n=10), mildly (n=10), and severely (n=10) calcified arteries. Results showed that calcification of the tunica media of mainly the pulmonary artery branches, was present in 82% of horses, and both breeds and genders were similarly affected. Lesions appeared as white-to-yellowish, hard, gritty plaques of variable size. Microscopically, elastic fibers were thin, fragmented and calcified, and surrounded by dense collagen matrix, as described for Mönckeberg sclerosis. Elemental analysis of the calcified areas was consistent with hydroxyapatite mineral. No immunoreactivity for the smooth muscle cell markers, smooth muscle α-actin and SM22α was observed in cells found at the calcification site. Many of these cells had a chondrocytic phenotype appearance and showed immunoreactivity for Sox9, a chondrocyte marker. Arterial calcification in horses share histopathological features with arterial medial calcification in humans and may result in similar physiological abnormalities such as vascular stiffness. The occurrence of VC in young racing horses indicates the need to investigate its pathogenesis and potential clinical implications.https://www.atlantis-press.com/article/125927375/view |
| spellingShingle | L.G. Arroyo M.A. Hayes L. Viel P10.08 PULMONARY ARTERY CALCIFICATION IN RACEHORSES Artery Research |
| title | P10.08 PULMONARY ARTERY CALCIFICATION IN RACEHORSES |
| title_full | P10.08 PULMONARY ARTERY CALCIFICATION IN RACEHORSES |
| title_fullStr | P10.08 PULMONARY ARTERY CALCIFICATION IN RACEHORSES |
| title_full_unstemmed | P10.08 PULMONARY ARTERY CALCIFICATION IN RACEHORSES |
| title_short | P10.08 PULMONARY ARTERY CALCIFICATION IN RACEHORSES |
| title_sort | p10 08 pulmonary artery calcification in racehorses |
| url | https://www.atlantis-press.com/article/125927375/view |
| work_keys_str_mv | AT lgarroyo p1008pulmonaryarterycalcificationinracehorses AT mahayes p1008pulmonaryarterycalcificationinracehorses AT lviel p1008pulmonaryarterycalcificationinracehorses |