Silver Nanoparticles and Graphene Oxide Complex as an Anti-Inflammatory Biocompatible Liquid Nano-Dressing for Skin Infected with Staphylococcus aureus
Marlena Zielińska-Górska,1 Malwina Sosnowska-Ławnicka,1 Sławomir Jaworski,1 Agata Lange,1 Karolina Daniluk,1 Barbara Nasiłowska,2 Bartosz Bartosewicz,2 André Chwalibog,3 Ewa Sawosz1 1Department of Nanobiotechnology, Institute of Biology, Warsaw University...
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| Format: | Article |
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Dove Medical Press
2023-11-01
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| Series: | Journal of Inflammation Research |
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| Online Access: | https://www.dovepress.com/silver-nanoparticles-and-graphene-oxide-complex-as-an-anti-inflammator-peer-reviewed-fulltext-article-JIR |
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| author | Zielińska-Górska M Sosnowska-Ławnicka M Jaworski S Lange A Daniluk K Nasiłowska B Bartosewicz B Chwalibog A Sawosz E |
| author_facet | Zielińska-Górska M Sosnowska-Ławnicka M Jaworski S Lange A Daniluk K Nasiłowska B Bartosewicz B Chwalibog A Sawosz E |
| author_sort | Zielińska-Górska M |
| collection | DOAJ |
| description | Marlena Zielińska-Górska,1 Malwina Sosnowska-Ławnicka,1 Sławomir Jaworski,1 Agata Lange,1 Karolina Daniluk,1 Barbara Nasiłowska,2 Bartosz Bartosewicz,2 André Chwalibog,3 Ewa Sawosz1 1Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, 02-787, Poland; 2Institute of Optoelectronics, Military University of Technology, Warsaw, 00-908, Poland; 3Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, 1870, DenmarkCorrespondence: Marlena Zielińska-Górska, Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Ciszewskiego 8 St., bldg. 23, room 110, Warsaw, 02-786, Poland, Tel +48225936665, Email marlena_zielinska_gorska@sggw.edu.plBackground: Bacterial skin infections, including Staphylococcus aureus, are a powerful and still not fully resolved problem. The aim of this research was to determine the possibility of using a complex of graphene oxide (GO) encrusted with silver nanoparticles as an effective antibacterial agent against S. aureus and to assess its pro-inflammatory properties.Methods: The tests were carried out in vitro on EpiDerm™ Skin, an artificial skin model (MatTek in vitro Life Science Laboratories, Slovak Republic), and the fibroblast cell line (HFF-2 from ATCC, USA). Both models were infected with S. aureus bacteria (ATCC 25923) and then treated with antibiotics or our experimental factors: silver nanoparticles (AgNPs, Nano-koloid, Poland), graphene oxide (GO, NanoPoz, Poland), and complex AgNP-GO (hydrocolloid created by self-assembly).Results: The antibacterial effectiveness of the AgNP-GO complex was equivalent to that of the antibiotic. In addition, an increase in the level of pro-inflammatory cytokines was observed under the influence of antibiotic administration, in contrast to the effect of AgNP-GO, which showed very limited pro-inflammatory activity.Conclusion: Hydrocolloid of the AgNP-GO complex, administered in the form of a liquid dressing, may act as an antibacterial agent and also reduce inflammation induced by S. aureus infection.Keywords: human skin, inflammation state, nanomaterials, antibacterial properties |
| first_indexed | 2024-03-09T23:32:39Z |
| format | Article |
| id | doaj.art-e783cea28ddf4e25aa4dba9006160b0f |
| institution | Directory Open Access Journal |
| issn | 1178-7031 |
| language | English |
| last_indexed | 2024-03-09T23:32:39Z |
| publishDate | 2023-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Inflammation Research |
| spelling | doaj.art-e783cea28ddf4e25aa4dba9006160b0f2023-11-23T17:07:55ZengDove Medical PressJournal of Inflammation Research1178-70312023-11-01Volume 165477549388392Silver Nanoparticles and Graphene Oxide Complex as an Anti-Inflammatory Biocompatible Liquid Nano-Dressing for Skin Infected with Staphylococcus aureusZielińska-Górska MSosnowska-Ławnicka MJaworski SLange ADaniluk KNasiłowska BBartosewicz BChwalibog ASawosz EMarlena Zielińska-Górska,1 Malwina Sosnowska-Ławnicka,1 Sławomir Jaworski,1 Agata Lange,1 Karolina Daniluk,1 Barbara Nasiłowska,2 Bartosz Bartosewicz,2 André Chwalibog,3 Ewa Sawosz1 1Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, 02-787, Poland; 2Institute of Optoelectronics, Military University of Technology, Warsaw, 00-908, Poland; 3Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, 1870, DenmarkCorrespondence: Marlena Zielińska-Górska, Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Ciszewskiego 8 St., bldg. 23, room 110, Warsaw, 02-786, Poland, Tel +48225936665, Email marlena_zielinska_gorska@sggw.edu.plBackground: Bacterial skin infections, including Staphylococcus aureus, are a powerful and still not fully resolved problem. The aim of this research was to determine the possibility of using a complex of graphene oxide (GO) encrusted with silver nanoparticles as an effective antibacterial agent against S. aureus and to assess its pro-inflammatory properties.Methods: The tests were carried out in vitro on EpiDerm™ Skin, an artificial skin model (MatTek in vitro Life Science Laboratories, Slovak Republic), and the fibroblast cell line (HFF-2 from ATCC, USA). Both models were infected with S. aureus bacteria (ATCC 25923) and then treated with antibiotics or our experimental factors: silver nanoparticles (AgNPs, Nano-koloid, Poland), graphene oxide (GO, NanoPoz, Poland), and complex AgNP-GO (hydrocolloid created by self-assembly).Results: The antibacterial effectiveness of the AgNP-GO complex was equivalent to that of the antibiotic. In addition, an increase in the level of pro-inflammatory cytokines was observed under the influence of antibiotic administration, in contrast to the effect of AgNP-GO, which showed very limited pro-inflammatory activity.Conclusion: Hydrocolloid of the AgNP-GO complex, administered in the form of a liquid dressing, may act as an antibacterial agent and also reduce inflammation induced by S. aureus infection.Keywords: human skin, inflammation state, nanomaterials, antibacterial propertieshttps://www.dovepress.com/silver-nanoparticles-and-graphene-oxide-complex-as-an-anti-inflammator-peer-reviewed-fulltext-article-JIRhuman skininflammation statenanomaterialsantibacterial properties |
| spellingShingle | Zielińska-Górska M Sosnowska-Ławnicka M Jaworski S Lange A Daniluk K Nasiłowska B Bartosewicz B Chwalibog A Sawosz E Silver Nanoparticles and Graphene Oxide Complex as an Anti-Inflammatory Biocompatible Liquid Nano-Dressing for Skin Infected with Staphylococcus aureus Journal of Inflammation Research human skin inflammation state nanomaterials antibacterial properties |
| title | Silver Nanoparticles and Graphene Oxide Complex as an Anti-Inflammatory Biocompatible Liquid Nano-Dressing for Skin Infected with Staphylococcus aureus |
| title_full | Silver Nanoparticles and Graphene Oxide Complex as an Anti-Inflammatory Biocompatible Liquid Nano-Dressing for Skin Infected with Staphylococcus aureus |
| title_fullStr | Silver Nanoparticles and Graphene Oxide Complex as an Anti-Inflammatory Biocompatible Liquid Nano-Dressing for Skin Infected with Staphylococcus aureus |
| title_full_unstemmed | Silver Nanoparticles and Graphene Oxide Complex as an Anti-Inflammatory Biocompatible Liquid Nano-Dressing for Skin Infected with Staphylococcus aureus |
| title_short | Silver Nanoparticles and Graphene Oxide Complex as an Anti-Inflammatory Biocompatible Liquid Nano-Dressing for Skin Infected with Staphylococcus aureus |
| title_sort | silver nanoparticles and graphene oxide complex as an anti inflammatory biocompatible liquid nano dressing for skin infected with staphylococcus aureus |
| topic | human skin inflammation state nanomaterials antibacterial properties |
| url | https://www.dovepress.com/silver-nanoparticles-and-graphene-oxide-complex-as-an-anti-inflammator-peer-reviewed-fulltext-article-JIR |
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