<i>PafS</i> Containing GGDEF-Domain Regulates Life Activities of <i>Pseudomonas glycinae</i> MS82
Cyclic dimeric guanosine monophosphate (c-di-GMP) is synthesized by diguanylate cyclase (DGC) with the GGDEF domain. As a ubiquitous bacterial second messenger, it regulates diverse life-activity phenotypes in some bacteria. Although 38 genes encoding GGDEF-domain-containing proteins have been ident...
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MDPI AG
2022-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/12/2342 |
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| author | Xianyi Chen Shaoxuan Qu Xin Luo Shi-En Lu Youzhou Liu Huiping Li Lijuan Hou Jinsheng Lin Ning Jiang Lin Ma |
| author_facet | Xianyi Chen Shaoxuan Qu Xin Luo Shi-En Lu Youzhou Liu Huiping Li Lijuan Hou Jinsheng Lin Ning Jiang Lin Ma |
| author_sort | Xianyi Chen |
| collection | DOAJ |
| description | Cyclic dimeric guanosine monophosphate (c-di-GMP) is synthesized by diguanylate cyclase (DGC) with the GGDEF domain. As a ubiquitous bacterial second messenger, it regulates diverse life-activity phenotypes in some bacteria. Although 38 genes encoding GGDEF-domain-containing proteins have been identified in the genome of the <i>Pseudomonas glycinae</i> strain MS82, whether c-di-GMP functions as a facilitator or repressor of life-activity phenotypes is poorly understood. In this study, one of the 38 genes containing a GGDEF domain in MS82, <i>PafS</i> was investigated to explore its regulatory function in bacterial life activities. The <i>PafS-</i>deletion mutant Δ<i>PafS</i> and reversion mutant <i>PafS</i>-comp were constructed by the method of biparental conjugation and homologous recombination. The life activities of the mutants, such as antifungal activity, biofilm formation ability, polysaccharide content, and motor behavior, were explored. The results showed that all life-activity phenotypes were significantly reduced after knocking out <i>PafS</i>, whereas all were significantly restored to a similar level to that of MS82 after the complementation of <i>PafS</i>. These results suggested that <i>PafS</i> plays an important role in the regulation of a range of cellular activities by c-di-GMP in <i>P. glycinae</i> MS82. |
| first_indexed | 2024-03-09T16:04:40Z |
| format | Article |
| id | doaj.art-e78beccfee9940d3bad1808b5b54e843 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:04:40Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-e78beccfee9940d3bad1808b5b54e8432023-11-24T16:47:30ZengMDPI AGMicroorganisms2076-26072022-11-011012234210.3390/microorganisms10122342<i>PafS</i> Containing GGDEF-Domain Regulates Life Activities of <i>Pseudomonas glycinae</i> MS82Xianyi Chen0Shaoxuan Qu1Xin Luo2Shi-En Lu3Youzhou Liu4Huiping Li5Lijuan Hou6Jinsheng Lin7Ning Jiang8Lin Ma9Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaDepartment of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, MS 39762, USAInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaJiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaCyclic dimeric guanosine monophosphate (c-di-GMP) is synthesized by diguanylate cyclase (DGC) with the GGDEF domain. As a ubiquitous bacterial second messenger, it regulates diverse life-activity phenotypes in some bacteria. Although 38 genes encoding GGDEF-domain-containing proteins have been identified in the genome of the <i>Pseudomonas glycinae</i> strain MS82, whether c-di-GMP functions as a facilitator or repressor of life-activity phenotypes is poorly understood. In this study, one of the 38 genes containing a GGDEF domain in MS82, <i>PafS</i> was investigated to explore its regulatory function in bacterial life activities. The <i>PafS-</i>deletion mutant Δ<i>PafS</i> and reversion mutant <i>PafS</i>-comp were constructed by the method of biparental conjugation and homologous recombination. The life activities of the mutants, such as antifungal activity, biofilm formation ability, polysaccharide content, and motor behavior, were explored. The results showed that all life-activity phenotypes were significantly reduced after knocking out <i>PafS</i>, whereas all were significantly restored to a similar level to that of MS82 after the complementation of <i>PafS</i>. These results suggested that <i>PafS</i> plays an important role in the regulation of a range of cellular activities by c-di-GMP in <i>P. glycinae</i> MS82.https://www.mdpi.com/2076-2607/10/12/2342<i>Pseudomonas glycinae</i> MS82GGDEF domain<i>PafS</i>life activities |
| spellingShingle | Xianyi Chen Shaoxuan Qu Xin Luo Shi-En Lu Youzhou Liu Huiping Li Lijuan Hou Jinsheng Lin Ning Jiang Lin Ma <i>PafS</i> Containing GGDEF-Domain Regulates Life Activities of <i>Pseudomonas glycinae</i> MS82 Microorganisms <i>Pseudomonas glycinae</i> MS82 GGDEF domain <i>PafS</i> life activities |
| title | <i>PafS</i> Containing GGDEF-Domain Regulates Life Activities of <i>Pseudomonas glycinae</i> MS82 |
| title_full | <i>PafS</i> Containing GGDEF-Domain Regulates Life Activities of <i>Pseudomonas glycinae</i> MS82 |
| title_fullStr | <i>PafS</i> Containing GGDEF-Domain Regulates Life Activities of <i>Pseudomonas glycinae</i> MS82 |
| title_full_unstemmed | <i>PafS</i> Containing GGDEF-Domain Regulates Life Activities of <i>Pseudomonas glycinae</i> MS82 |
| title_short | <i>PafS</i> Containing GGDEF-Domain Regulates Life Activities of <i>Pseudomonas glycinae</i> MS82 |
| title_sort | i pafs i containing ggdef domain regulates life activities of i pseudomonas glycinae i ms82 |
| topic | <i>Pseudomonas glycinae</i> MS82 GGDEF domain <i>PafS</i> life activities |
| url | https://www.mdpi.com/2076-2607/10/12/2342 |
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