Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats
Background/purpose: Autophagy, a lysosome-based degradation pathway that is reportedly activated by mechanical stress and nutrient deprivation, plays an important role in various physiological and pathological events. The present study investigated the level of autophagy and tumor necrosis factor-α(...
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Elsevier
2020-09-01
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| Series: | Journal of Dental Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790220300258 |
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| author | Jie Xu Xian Zhao Jin Zeng Jing-Hong Yu Simon Guan Xiao-Mei Xu Li Mei |
| author_facet | Jie Xu Xian Zhao Jin Zeng Jing-Hong Yu Simon Guan Xiao-Mei Xu Li Mei |
| author_sort | Jie Xu |
| collection | DOAJ |
| description | Background/purpose: Autophagy, a lysosome-based degradation pathway that is reportedly activated by mechanical stress and nutrient deprivation, plays an important role in various physiological and pathological events. The present study investigated the level of autophagy and tumor necrosis factor-α(TNF-α) expression in the periodontal ligaments (PDLs) of Sprague–Dawley (SD) rats to analyze the involvement of autophagy and inflammatory cytokines in orthodontic tooth movement (OTM) and maintaining periodontal tissue homeostasis. Materials and methods: SD rats (n = 100) were randomly divided into a control group (n = 10) and an experimental group (n = 90). An orthodontic appliance was placed in each rat in the experimental group, and 10 rats were randomly euthanized 15 min, 30 min, 1 h, 2 h, 4 h, 12 h, 1 d, 3 d and 7 d after mechanical loading. The OTM distance was then measured. Hematoxylin and eosin (HE) staining was used to analyze the morphology of the PDL. Immunohistochemical (IHC) staining and tartrate-resistant acid phosphatase (TRAP) staining were also performed. Results: After the application of orthodontic force and under the dual effects of mechanical force and starvation caused by compressed vessels, the level of autophagy and TNF-α expression in the PDL fluctuated and exhibited a similar trend. Conclusion: Our data suggest a significant correlation between the initiation of autophagy and TNF-α expression, which both exerted positive effects on PDL remodeling during OTM in rats. |
| first_indexed | 2024-12-22T15:11:18Z |
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| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-12-22T15:11:18Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-e791069fc9684ae591d83c3adac6afb62022-12-21T18:21:51ZengElsevierJournal of Dental Sciences1991-79022020-09-01153351363Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in ratsJie Xu0Xian Zhao1Jin Zeng2Jing-Hong Yu3Simon Guan4Xiao-Mei Xu5Li Mei6Department of Orthodontics, The Affiliated Stomatology Hospital of Southwest Medical University, Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou, Sichuan, PR ChinaDepartment of Orthodontics, The Affiliated Stomatology Hospital of Southwest Medical University, Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou, Sichuan, PR ChinaDepartment of Orthodontics, The Affiliated Stomatology Hospital of Southwest Medical University, Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou, Sichuan, PR ChinaDepartment of Orthodontics, The Affiliated Stomatology Hospital of Southwest Medical University, Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou, Sichuan, PR ChinaDepartment of Oral Diagnostic and Surgical Sciences, Faculty of Dentistry, University of Otago, New ZealandDepartment of Orthodontics, The Affiliated Stomatology Hospital of Southwest Medical University, Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou, Sichuan, PR China; Corresponding author. Department of Orthodontics, The Affiliated Stomatology Hospital of Southwest Medical University, Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, 2 Jiangyang South Road, Luzhou, Sichuan, 646000, PR China.Discipline of Orthodontics, Department of Oral Science, Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New ZealandBackground/purpose: Autophagy, a lysosome-based degradation pathway that is reportedly activated by mechanical stress and nutrient deprivation, plays an important role in various physiological and pathological events. The present study investigated the level of autophagy and tumor necrosis factor-α(TNF-α) expression in the periodontal ligaments (PDLs) of Sprague–Dawley (SD) rats to analyze the involvement of autophagy and inflammatory cytokines in orthodontic tooth movement (OTM) and maintaining periodontal tissue homeostasis. Materials and methods: SD rats (n = 100) were randomly divided into a control group (n = 10) and an experimental group (n = 90). An orthodontic appliance was placed in each rat in the experimental group, and 10 rats were randomly euthanized 15 min, 30 min, 1 h, 2 h, 4 h, 12 h, 1 d, 3 d and 7 d after mechanical loading. The OTM distance was then measured. Hematoxylin and eosin (HE) staining was used to analyze the morphology of the PDL. Immunohistochemical (IHC) staining and tartrate-resistant acid phosphatase (TRAP) staining were also performed. Results: After the application of orthodontic force and under the dual effects of mechanical force and starvation caused by compressed vessels, the level of autophagy and TNF-α expression in the PDL fluctuated and exhibited a similar trend. Conclusion: Our data suggest a significant correlation between the initiation of autophagy and TNF-α expression, which both exerted positive effects on PDL remodeling during OTM in rats.http://www.sciencedirect.com/science/article/pii/S1991790220300258AutophagyMechanical stressOrthodontic tooth movementPeriodontal ligamentTNF-α |
| spellingShingle | Jie Xu Xian Zhao Jin Zeng Jing-Hong Yu Simon Guan Xiao-Mei Xu Li Mei Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats Journal of Dental Sciences Autophagy Mechanical stress Orthodontic tooth movement Periodontal ligament TNF-α |
| title | Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats |
| title_full | Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats |
| title_fullStr | Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats |
| title_full_unstemmed | Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats |
| title_short | Role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats |
| title_sort | role of autophagy in the periodontal ligament reconstruction during orthodontic tooth movement in rats |
| topic | Autophagy Mechanical stress Orthodontic tooth movement Periodontal ligament TNF-α |
| url | http://www.sciencedirect.com/science/article/pii/S1991790220300258 |
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