Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats
Objective: To investigate the effect of epigallocatechin-3-gallate (EGCG) on endothelial dysfunction in spontaneously hypertensive rats (SHR). Methods: Wistar-Kyoto (WKY) rats and SHR were divided into four groups; WKY control, SHR control and SHR treated with EGCG (50 mg/kg/day) or losartan (10 mg/...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=7;spage=287;epage=295;aulast=Khor |
| _version_ | 1797649603151003648 |
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| author | Yucinda YY Khor Siew-Keah Lee M Dharmani Devi Wei Chih Ling |
| author_facet | Yucinda YY Khor Siew-Keah Lee M Dharmani Devi Wei Chih Ling |
| author_sort | Yucinda YY Khor |
| collection | DOAJ |
| description | Objective: To investigate the effect of epigallocatechin-3-gallate (EGCG) on endothelial dysfunction in spontaneously hypertensive rats (SHR).
Methods: Wistar-Kyoto (WKY) rats and SHR were divided into four groups; WKY control, SHR control and SHR treated with EGCG (50 mg/kg/day) or losartan (10 mg/kg/day). The treatment was given daily for 4 weeks by oral gavage and the blood pressure was monitored by tail-cuff method every 3 days. Acetylcholine-induced endothelium-dependent relaxations were assessed in isolated phenylephrine-precontracted aortic rings at the end of treatment. The vascular levels of reactive oxygen species, nitric oxide, tetrahydrobiopterin, and cyclic guanosine monophosphate were also measured. Moreover, the expression of angiotensin II type 1 (AT1) receptor protein was determined.
Results: The systolic blood pressure was significantly decreased in SHR treated with EGCG. The impaired endothelium-dependent relaxation was significantly improved in aortic ring isolated from the EGCG-treated SHR group. EGCG also significantly increased the levels of nitric oxide, tetrahydrobiopterin, and cyclic guanosine monophosphate, while decreasing the level of reactive oxygen species and the protein expression of AT1 receptor in SHR.
Conclusions: EGCG attenuates endothelial dysfunction in SHR by decreasing oxidative stress and increasing vascular nitric oxide bioavailability, which may be modulated partly by inhibition of vascular AT1 receptors. An increase in endothelium-dependent relaxation may contribute to a decrease in blood pressure in hypertensive animals. |
| first_indexed | 2024-03-11T15:49:27Z |
| format | Article |
| id | doaj.art-e792308dbd364a07bf7972d2b45eca26 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 2588-9222 |
| language | English |
| last_indexed | 2024-03-11T15:49:27Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-e792308dbd364a07bf7972d2b45eca262023-10-26T05:32:07ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912588-92222023-01-0113728729510.4103/2221-1691.380560Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive ratsYucinda YY KhorSiew-Keah LeeM Dharmani DeviWei Chih LingObjective: To investigate the effect of epigallocatechin-3-gallate (EGCG) on endothelial dysfunction in spontaneously hypertensive rats (SHR). Methods: Wistar-Kyoto (WKY) rats and SHR were divided into four groups; WKY control, SHR control and SHR treated with EGCG (50 mg/kg/day) or losartan (10 mg/kg/day). The treatment was given daily for 4 weeks by oral gavage and the blood pressure was monitored by tail-cuff method every 3 days. Acetylcholine-induced endothelium-dependent relaxations were assessed in isolated phenylephrine-precontracted aortic rings at the end of treatment. The vascular levels of reactive oxygen species, nitric oxide, tetrahydrobiopterin, and cyclic guanosine monophosphate were also measured. Moreover, the expression of angiotensin II type 1 (AT1) receptor protein was determined. Results: The systolic blood pressure was significantly decreased in SHR treated with EGCG. The impaired endothelium-dependent relaxation was significantly improved in aortic ring isolated from the EGCG-treated SHR group. EGCG also significantly increased the levels of nitric oxide, tetrahydrobiopterin, and cyclic guanosine monophosphate, while decreasing the level of reactive oxygen species and the protein expression of AT1 receptor in SHR. Conclusions: EGCG attenuates endothelial dysfunction in SHR by decreasing oxidative stress and increasing vascular nitric oxide bioavailability, which may be modulated partly by inhibition of vascular AT1 receptors. An increase in endothelium-dependent relaxation may contribute to a decrease in blood pressure in hypertensive animals.http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=7;spage=287;epage=295;aulast=Khorepigallocatechin-3-gallate; vascular protection; antihypertension; tetrahydrobiopterin; cyclic guanosine monophosphate; angiotensin type i receptor; spontaneously hypertensive rats |
| spellingShingle | Yucinda YY Khor Siew-Keah Lee M Dharmani Devi Wei Chih Ling Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats Asian Pacific Journal of Tropical Biomedicine epigallocatechin-3-gallate; vascular protection; antihypertension; tetrahydrobiopterin; cyclic guanosine monophosphate; angiotensin type i receptor; spontaneously hypertensive rats |
| title | Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats |
| title_full | Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats |
| title_fullStr | Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats |
| title_full_unstemmed | Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats |
| title_short | Epigallocatechin-3-gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats |
| title_sort | epigallocatechin 3 gallate exerts antihypertensive effects and improves endothelial function in spontaneously hypertensive rats |
| topic | epigallocatechin-3-gallate; vascular protection; antihypertension; tetrahydrobiopterin; cyclic guanosine monophosphate; angiotensin type i receptor; spontaneously hypertensive rats |
| url | http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=7;spage=287;epage=295;aulast=Khor |
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