Identification of Differentially-expressed Genes in Intestinal Gastric Cancer by Microarray Analysis
Gastric cancer (GC) is one of the most frequent malignant tumors. In order to systematically characterize the cellular and molecular mechanisms of intestinal GC development, in this study, we used 22 K oligonucleotide microarrays and bioinformatics analysis to evaluate the gene expression profiles o...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2014-12-01
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| Series: | Genomics, Proteomics & Bioinformatics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672022914001338 |
| _version_ | 1827158861601046528 |
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| author | Shizhu Zang Ruifang Guo Rui Xing Liang Zhang Wenmei Li Min Zhao Jingyuan Fang Fulian Hu Bin Kang Yonghong Ren Yonglong Zhuang Siqi Liu Rong Wang Xianghong Li Yingyan Yu Jing Cheng Youyong Lu |
| author_facet | Shizhu Zang Ruifang Guo Rui Xing Liang Zhang Wenmei Li Min Zhao Jingyuan Fang Fulian Hu Bin Kang Yonghong Ren Yonglong Zhuang Siqi Liu Rong Wang Xianghong Li Yingyan Yu Jing Cheng Youyong Lu |
| author_sort | Shizhu Zang |
| collection | DOAJ |
| description | Gastric cancer (GC) is one of the most frequent malignant tumors. In order to systematically characterize the cellular and molecular mechanisms of intestinal GC development, in this study, we used 22 K oligonucleotide microarrays and bioinformatics analysis to evaluate the gene expression profiles of GC in 45 tissue samples, including 20 intestinal GC tissue samples, 20 normal appearing tissues (NATs) adjacent to tumors and 5 noncancerous gastric mucosa tissue samples. These profiles allowed us to explore the transcriptional characteristics of GC and determine the change patterns in gene expression that may be of clinical significance. 1519 and 1255 differentially-expressed genes (DEGs) were identified in intestinal GC tissues and NATs, respectively, as determined by Bayesian analysis (P < 0.001). These genes were associated with diverse functions such as mucosa secretion, metabolism, proliferation, signaling and development, which occur at different stages of GC development. |
| first_indexed | 2024-03-08T07:52:17Z |
| format | Article |
| id | doaj.art-e792c9d2fa40406092d51492577bc3f0 |
| institution | Directory Open Access Journal |
| issn | 1672-0229 |
| language | English |
| last_indexed | 2025-03-20T23:47:58Z |
| publishDate | 2014-12-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Genomics, Proteomics & Bioinformatics |
| spelling | doaj.art-e792c9d2fa40406092d51492577bc3f02024-08-03T12:42:22ZengOxford University PressGenomics, Proteomics & Bioinformatics1672-02292014-12-0112627628310.1016/j.gpb.2014.09.004Identification of Differentially-expressed Genes in Intestinal Gastric Cancer by Microarray AnalysisShizhu Zang0Ruifang Guo1Rui Xing2Liang Zhang3Wenmei Li4Min Zhao5Jingyuan Fang6Fulian Hu7Bin Kang8Yonghong Ren9Yonglong Zhuang10Siqi Liu11Rong Wang12Xianghong Li13Yingyan Yu14Jing Cheng15Youyong Lu16Laboratory of Molecular Oncology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaLaboratory of Molecular Oncology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaLaboratory of Molecular Oncology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaNational Engineering Research Center for Beijing Biochip Technology and Tsinghua University School of Medicine, Beijing 102206, ChinaLaboratory of Molecular Oncology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaLaboratory of Molecular Oncology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaDepartment of Gastroenterology, Renji Hospital of Shanghai Second Medical University, Shanghai 200001, ChinaDepartment of Gastroenterology, Peking University First Hospital, Beijing 100034, ChinaLaboratory of Molecular Oncology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaNational Engineering Research Center for Beijing Biochip Technology and Tsinghua University School of Medicine, Beijing 102206, ChinaNational Engineering Research Center for Beijing Biochip Technology and Tsinghua University School of Medicine, Beijing 102206, ChinaBeijing Institutes of Genomics, Chinese Academy of Sciences, Beijing 100101, ChinaDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York 10029, USADepartment of Pathology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaShanghai Ruijin Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200025, ChinaNational Engineering Research Center for Beijing Biochip Technology and Tsinghua University School of Medicine, Beijing 102206, ChinaLaboratory of Molecular Oncology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, ChinaGastric cancer (GC) is one of the most frequent malignant tumors. In order to systematically characterize the cellular and molecular mechanisms of intestinal GC development, in this study, we used 22 K oligonucleotide microarrays and bioinformatics analysis to evaluate the gene expression profiles of GC in 45 tissue samples, including 20 intestinal GC tissue samples, 20 normal appearing tissues (NATs) adjacent to tumors and 5 noncancerous gastric mucosa tissue samples. These profiles allowed us to explore the transcriptional characteristics of GC and determine the change patterns in gene expression that may be of clinical significance. 1519 and 1255 differentially-expressed genes (DEGs) were identified in intestinal GC tissues and NATs, respectively, as determined by Bayesian analysis (P < 0.001). These genes were associated with diverse functions such as mucosa secretion, metabolism, proliferation, signaling and development, which occur at different stages of GC development.http://www.sciencedirect.com/science/article/pii/S1672022914001338Gastric cancer developmentMicroarrayGene expression profile |
| spellingShingle | Shizhu Zang Ruifang Guo Rui Xing Liang Zhang Wenmei Li Min Zhao Jingyuan Fang Fulian Hu Bin Kang Yonghong Ren Yonglong Zhuang Siqi Liu Rong Wang Xianghong Li Yingyan Yu Jing Cheng Youyong Lu Identification of Differentially-expressed Genes in Intestinal Gastric Cancer by Microarray Analysis Genomics, Proteomics & Bioinformatics Gastric cancer development Microarray Gene expression profile |
| title | Identification of Differentially-expressed Genes in Intestinal Gastric Cancer by Microarray Analysis |
| title_full | Identification of Differentially-expressed Genes in Intestinal Gastric Cancer by Microarray Analysis |
| title_fullStr | Identification of Differentially-expressed Genes in Intestinal Gastric Cancer by Microarray Analysis |
| title_full_unstemmed | Identification of Differentially-expressed Genes in Intestinal Gastric Cancer by Microarray Analysis |
| title_short | Identification of Differentially-expressed Genes in Intestinal Gastric Cancer by Microarray Analysis |
| title_sort | identification of differentially expressed genes in intestinal gastric cancer by microarray analysis |
| topic | Gastric cancer development Microarray Gene expression profile |
| url | http://www.sciencedirect.com/science/article/pii/S1672022914001338 |
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